Study on Carcass Contaminating Escherichia coli in Apparently Healthy Slaughtered Cattle in Haramaya University Slaughter House with Special Emphasis on Escherichia coli O157:H7, Ethiopia

Terefe, Dechassa

doi:10.4172/2157-7579.1000132

Cited by 14 publications

(15 citation statements)

References 19 publications

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“…Similar findings on multidrug resistance of E. coli strains has been reported from Ethiopia [38, 39, 51] and other parts of the world (Khan et al 2002; [33, 37, 43, 44, 55, 56]). Such high incidence of multidrug resistance may apparently have occurred due to indiscriminate utilization of antimicrobial agents which may ultimately replace the susceptible microorganisms [53, 57].…”

Section: Discussionsupporting

confidence: 85%

“…Similar to the current finding Hailu and Tefera [38] had reported susceptible E. coli O157:H7 isolates to chloramphenicol (100%), spectinomycin (62.61%), and nalidixic acid (61.76%). Taye et al [39] also reported most isolated strains were found susceptible to chloramphenicol and spectinomycin. Closely related chloramphenicol susceptibility to our finding was also reported by Hamisi et al [13] and Talebiyan et al [40] from Tanzania and Iran, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Cloacael Carriage and Multidrug Resistance Escherichia coli O157:H7 from Poultry Farms, Eastern Ethiopia

Shecho

Thomas

Kemal

et al. 2017

Journal of Veterinary Medicine

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A cross-sectional study was carried out to determine antimicrobial drug resistance patterns of E. coli O157:H7 isolates and estimate the level of the pathogen. A total of 194 cloacae swab samples were collected randomly in two poultry farms. Standard cultural, biochemical, and serological (latex agglutination) methods were used to isolate E. coli O157:H7. The isolates were subjected to antimicrobial susceptibility testing using disc diffusion method. Out of 194 cloacae samples examined, 13.4% (n = 26) were found to be positive for E. coli O157:H7. The finding indicated differences in E. coli O157:H7 infection among the different risk factors. Chicken from Adele Poultry Farm showed higher E. coli O157:H7 infection (OR = 3.89) than Haramaya University poultry farm and young birds had more infection (OR = 4.62) than adult birds. Of the total 14 antimicrobials included in the panel of study, the susceptibility results were varied with 96.15% and 0% E. coli O157:H7 isolates expressing resistance to erythromycin, clindamycin, spectinomycin, and ciprofloxacin, respectively. Multidrug resistance to more than two antimicrobial agents was detected in 24 (92.30%) of the isolates. The study showed high presence of antimicrobial resistant isolates of E. coli O157:H7. Further study is required to better understand the ecology and evolution of bacterial resistance to antimicrobial agents.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Cloacael Carriage and Multidrug Resistance Escherichia coli O157:H7 from Poultry Farms, Eastern Ethiopia

Shecho

Thomas

Kemal

et al. 2017

Journal of Veterinary Medicine

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“…Accordingly, E. coli were detected in 102 (20.2%) of the samples tested, whereas E. coli O157: H7 were detected in 27 (5.4%) of the samples. The proportion of positive samples in this study was higher than the previous study nding in different parts of Ethiopia [8,[14][15][16][17][18][19][20]. However, this study nding is lower than the study nding in Ethiopia and other country [9,21,22].…”

Section: Resultscontrasting

confidence: 79%

“…With regard to samples types, higher proportions of ceacal contents of slaughtered animals were positive for E. coli and E. coli O157: H7 followed by meat and other different swab samples obtained from abattoir and butcher shops. The presence of E. coli and E. coli O157: H7 in meat samples, ceacal content and swab samples were also reported in different studies in Ethiopia [9,[14][15][16][17][18][19][20][21][23][24][25][26][27]. Many reasons might be contributed for crosscontaminations of meat and surface contacts with ceacal contents and other sources.…”

Section: Resultsmentioning

confidence: 87%

Occurrence of multi-drug resistant Escherichia coli and Escherichia coli O157:H7 in meat and swab samples of various contact surfaces at abattoir and butcher shops in Jimma town, Southwest district of Ethiopia

Abayneh

Asfaw

2020

Preprint

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Background: Raw meat is one of commonly consumed traditional diets in Ethiopia. However, unhygienic processing and distribution practices were risky for contaminations of meat leads to human infection. This study was conducted to assess the presence of multi-drug resistant E. coli and E. coli O157:H7 in meats, ceacal content, and swabs samples of different contact surfaces at abattoir and butcher shops in Jimma town, Southwest Ethiopia. Methodology: A cross-sectional descriptive study was conducted from April to July, 2018. The isolation and identification processes passed through enrichment of samples with modified tryptone soy broth (mTSB), streaked onto MacConkey agar and Cefixime- tellurite sorbitol MacConkey agar, biochemical testing (indole and TSI), followed by latex agglutination test. Results: Out of 505 samples, 102(20.2%) and 27(5.4%) were positive for E. coli and E. coli O157:H7, respectively. Of these, 55(19.3%) and 47 (21.4%) of E. coli and 17 (6.0%) and 10 (4.5%) of E. coli O157:H7 were isolated from the abattoir and butcher shop samples, respectively. A significant difference in the occurrences was observed among sample sources. Antimicrobials susceptibility test result showed that, 92.2% to 96.1% of E. coli and 85.5% to 96.3% of E. coli O157:H7 were susceptible to third generation cephalosporin, ciprofloxacin, gentamycin, kanamycin, streptomycin and chloramphenicol. About 91.2% and 97.1% of E. coli and 88.9 % and 92.6% of E. coli 0157:H7 were resistant to ampicillin and erythromycin, respectively. A total of 98 (76.0%) E. coli and E. coli O157:H7 isolates were resistant to two or more classes of antibiotics. All abattoir and butcher shops workers were not have any formal education and training certificate on food safety and unhygienic practice were also observed.Conclusion: The presence of E. coli and E. coli O157:H7 including multi-drugs resistant isolates in raw meat highlights how the current meat processing and distribution practice was unhygienic. Therefore, strategies in the prevention and control of food borne infections that could be caused by multi-drug resistant strains will depends much on hygienic processing and distribution practices of meat.

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“…In Ethiopia, there have been reports on the drug resistance of E. coli isolates from animal-derived food products [31, 32]. The highest drug resistance recorded in the current study might be due to high antimicrobial use in dairy farms, fruit juices, and individual cows to treat various diseases affecting the dairy sector.…”

Section: Discussionmentioning

confidence: 62%

Antimicrobial Resistance Profile ofE. coliIsolated from Raw Cow Milk and Fresh Fruit Juice in Mekelle, Tigray, Ethiopia

Tadesse

Gidey

Workelule

et al. 2018

Veterinary Medicine International

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Aim Foodborne illnesses represent a public health problem in developed and developing countries. They cause great suffering and are transmitted directly or indirectly between animals and humans and circulate in the global environment. E. coli are among them, causing a major public health problem. The aim of this study was therefore to study the antimicrobial resistance profile of E. coli from raw cow milk and fruit juice. Materials and Methods A cross-sectional study was conducted from October 2016 to June 2017 on 258 samples collected from milk shops (n = 86), dairy farms (n = 86), and fruit juice (n = 86) in different subcities of Mekelle. Bacteriological procedures were used for isolation of E. coli in the collected samples and for identification of the antimicrobial resistance profile. Result The overall mean viable bacterial count and standard deviation of samples from milk shop, fruit juice, and dairy milk were found to be 8.86 ± 107, 7.2 ± 107, and 8.65 ± 107 CFU/ml and 33.87 ± 106, 6.68 ± 106, and 22.0 ± 106, respectively. Of the samples tested, 39 from milk shops (45.35%), 20 from fruit juice (23.26%), and 24 from dairy farms (27.91%) were found to be positive for E. coli. The isolated E. coli were highly resistant to ampicillin (70%), sulfamethoxazole-trimethoprim (60%), clindamycin (80%), erythromycin (60%), chloramphenicol (50%), and kanamycin (50%) and were found to be susceptible to some antibiotics like gentamicin (100%), norfloxacin (100%), tetracycline (60%), polymyxin B (90%), and ciprofloxacin (90%). Conclusion The current study supports the finding that raw milk and fruit juice can be regarded as critical source of pathogenic E. coli. This supports the need for strict monitoring and the implementation of effective hygienic and biosecurity measures in the whole food chain of these products as well as a prudent use of antimicrobials.

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Study on Carcass Contaminating Escherichia coli in Apparently Healthy Slaughtered Cattle in Haramaya University Slaughter House with Special Emphasis on Escherichia coli O157:H7, Ethiopia

Cited by 14 publications

References 19 publications

Cloacael Carriage and Multidrug Resistance Escherichia coli O157:H7 from Poultry Farms, Eastern Ethiopia

Cloacael Carriage and Multidrug Resistance Escherichia coli O157:H7 from Poultry Farms, Eastern Ethiopia

Occurrence of multi-drug resistant Escherichia coli and Escherichia coli O157:H7 in meat and swab samples of various contact surfaces at abattoir and butcher shops in Jimma town, Southwest district of Ethiopia

Antimicrobial Resistance Profile ofE. coliIsolated from Raw Cow Milk and Fresh Fruit Juice in Mekelle, Tigray, Ethiopia

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