Prevalence of human T-cell lymphotropic virus types 1 and 2 in blood donors of the Caruaru Blood Center (Hemope)

Lima, Waleska Mayara Gomes de; Esteves, Fabrício Andrade Martins; Torres, Maria do Carmo Morais Rodrigues; Pires, Edna Suely Feitosa

doi:10.5581/1516-8484.20130061

Cited by 5 publications

(6 citation statements)

References 11 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, 3 out of 4 HTLV-1 seropositive donors were males. This is not in conformity with studies that have shown prevalence of HTLV-1 and HTLV-2 to be higher in females as opposed to males, which is related to transmission that is more efficient from men to women during sexual intercourse [18]. The high seroprevalence of HTLV-1 antibodies among male's blood donors than female is most likely due to the higher proportion of males compared with females in this study.…”

Section: Discussioncontrasting

confidence: 95%

See 1 more Smart Citation

Human T-cell lymphotropic virus types 1 and 2 antibodies seroprevalence among blood donors at national public health blood bank, Khartoum- Sudan 2019

Bmt¹

2020

J Med sci Res.

View full text Add to dashboard Cite

The human T-cell lymphotropic virus types 1 and 2 (HTLV 1/2) are well known causes of adult T cell leukemia lymphoma. Both viruses were established to be transmitted through various mode including sexual contact and blood transfusion. This study was aimed to determine the seroprevalence of HTLV-1/2 antibodies among blood donors in Public Health Laboratory. During the period of August 2019 to October 2019 a total of 394 blood samples were obtained from blood donors visiting the Blood bank of National Public Health Laboratory, both males and females were included. The blood samples were analyzed for the presence of anti - HTLV-1,2 by a commercially available enzyme-linked immune-sorbent assay following the instructions of the manufacturer. The study participants were included 361 (91.6%) males and 33 (8.4%) females. The result show that of the 394 blood donors, four (4) were found to be seropositive for HTLV-1 antibodies giving a prevalence of 1.02%. While all samples were negative for the HTLV-2 antibodies. Among HTLV-1 positive cases 3 were male (75%) while only one female (25%) was found to be seropositive for HTLV-1 antibodies. None of the married donors was found to be seropositive for HTLV-1. We conclude that the seroprevalence of HTLV 1/2 were matched to the internationally estimated prevalence among blood donors at Blood Bank of national health laboratory and the majority of the cases were male under 40 years old. Further studies should be done with inclusion of more samples and using more sensitive technique like Western blot or PCR.

show abstract

Section: Discussioncontrasting

confidence: 95%

“…CO value for HTV-2 specific kit was calculated through the equation of (CO) =NC+0. 18 where NC is mean of the three negative controls = (0.04+0.031+0.01)/3 +0.18=0.25.…”

Section: Quality Control and Calculation Of The Resultsmentioning

confidence: 99%

Human T-cell lymphotropic virus types 1 and 2 antibodies seroprevalence among blood donors at national public health blood bank, Khartoum- Sudan 2019

Bmt¹

2020

J Med sci Res.

View full text Add to dashboard Cite

show abstract

“…In this study, all HTLV-1/2 seropositive donors were males. This is not in conformity with studies that have shown prevalence of HTLV-1 and HTLV-2 to be higher in females as opposed to males which is attributed to more efficient transmission from men to women during sexual intercourse [ 23 ].…”

Section: Discussioncontrasting

confidence: 90%

Human T-Cell Lymphotropic Virus Types 1 and 2 Seropositivity among Blood Donors at Mbarara Regional Blood Bank, South Western Uganda

Tweteise

Natukunda

Bazira

2016

Leukemia Research and Treatment

View full text Add to dashboard Cite

Background. The human T-cell lymphotropic virus types 1 and 2 (HTLV 1/2) are retroviruses associated with different pathologies. HTLV-1 causes adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP); HTLV-2 is not clearly associated with a known clinical disease. Both viruses may be transmitted by whole blood transfusion, from mother to child predominantly through breastfeeding, and by sexual contact. Presently, none of the regional blood banks in Uganda perform routine pretransfusion screening for HTLV. The aim of this study was to determine the prevalence of anti-human T-cell lymphotropic virus types 1/2 (HTLV-1/2) antibodies among blood donors at Mbarara Regional Blood Bank in South Western Uganda. A cross-sectional study was conducted between June 2014 and September 2014. Methodology. Consecutive blood samples of 368 blood donors were screened for anti-HTLV-1/2 antibodies using an enzyme linked immunosorbent assay (ELISA). Samples reactive on a first HTLV-1/2 ELISA were further retested in duplicate using the same ELISA. Of the three hundred and sixty-eight blood donors (229 (62.2%) males and 139 (37.8%) females), only two male donors aged 20 and 21 years were HTLV-1/2 seropositive, representing a prevalence of 0.54%. Conclusion. HTLV-1/2 prevalence is low among blood donors at Mbarara Regional Blood Bank. Studies among other categories of people at risk for HTLV 1/2 infection should be carried out.

show abstract

“…Furthermore, some false-positive reactions may be due to cross-reacting with anti-HLA antibodies, thus, it may not be reliable for the diagnosis of HTLV-1-associated acute infection. But this may be resolved by using western blot analysis techniques (Hamedi et al, 2012;Lima et al, 2013;Satake et al, 2016).…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Current approaches for detection of human T‐lymphotropic virus Type 1: A systematic review

Fani

Rezayi

Meshkat

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Background Human T‐lymphotropic virus Type 1 (HTLV‐1) is a retrovirus that is endemic in some regions of the world. It is known to cause several diseases like adult T‐cell leukemia (ATL) and HTLV‐1‐associated myelopathy/tropical spastic paraparesis (HAM/TSP). Serology and molecular methods have been used to detect this virus. Of these, enzyme‐linked immunosorbent assay (ELISA) is used as a primary screening method and this is usually followed by western blotting (WB) and polymerase chain reaction (PCR) methods as confirmatory tests. We conducted a systematic review of the different techniques used in the diagnosis of HTLV‐1 infection. Materials and Methods Our search was limited to original papers in the English language from 2010 to 2018 using several databases including Pubmed, Scopus, Google Scholar, Iranmedex, and Scientific Information Database. A manual search of references provided in the included papers was also performed. Results Of 101 electronically searched citations, 43 met the inclusion criteria. ELISA is commonly used for qualitative and screening detection, and WB and PCR techniques are used to confirm infection. Conclusion Among all the reported methods for detection of HTLV‐1, only serological and molecular tests are used as the most common technical assays for HTLV‐1. The ELISA assay, without a confirmatory test, has several limitations and affect the accuracy of the results. Owing to the prevalence of HTLV‐1 and limitations of the current detection methods, further evaluation of the accuracy of these methods is needed. There are new opportunities for applying novel technological advances in microfluidics, biosensors, and lab‐on‐a‐chip systems to perform HTLV‐1 diagnostics.

show abstract

Prevalence of human T-cell lymphotropic virus types 1 and 2 in blood donors of the Caruaru Blood Center (Hemope)

Cited by 5 publications

References 11 publications

Human T-cell lymphotropic virus types 1 and 2 antibodies seroprevalence among blood donors at national public health blood bank, Khartoum- Sudan 2019

Human T-cell lymphotropic virus types 1 and 2 antibodies seroprevalence among blood donors at national public health blood bank, Khartoum- Sudan 2019

Human T-Cell Lymphotropic Virus Types 1 and 2 Seropositivity among Blood Donors at Mbarara Regional Blood Bank, South Western Uganda

Current approaches for detection of human T‐lymphotropic virus Type 1: A systematic review

Contact Info

Product

Resources

About