Prevalence of Human Papillomavirus Types in High-Grade Cervical Intraepithelial Neoplasia and Cancer in Italy

Abstract

Background: The aim of this multicentric study was to identify human papillomavirus (HPV) type distribution in invasive cervical cancer and high-grade cervical intraepithelial neoplasia 2/3 (CIN2/3) in Italy.Methods: Cases were sampled through the electronic databases at the pathology units of eight centers in six regions from central and southern Italy. HPV types were detected from paraffin-embedded tissue samples and cervical specimens through amplification of HPV DNA with GP5+/GP6+ primers, followed by genotyping with reverse line blot (RLB). Untyped HPV-positive samples were sequenced. HPV-negative samples underwent nested PCR, followed by either RLB or sequencing. Finally, the remaining HPV-negative samples were amplified with primers targeting the virus E6 to E7 regions.Results: From 1,162 cases initially selected, 722 samples were further analyzed: 144 CIN2, 385 CIN3, 157 invasive squamous carcinomas, and 36 adenocarcinomas. Samples (6.9%) were HPV negative. The proportion of HPV16/18 was 60.8%, 76.6%, and 78.8% in CIN2, CIN3, and invasive cancers, respectively (P trend = 0.004). There was a significant decreasing trend of HPV16/18 with age in invasive cancers, going from 92% in women <35 years to 73% in women >55 years (P = 0.036). The proportion of coinfections was 16.8%, 15.5%, and 10.0% in CIN2, CIN3, and invasive cancers, respectively (P trend = 0.07).Conclusions: The proportion of invasive cancers caused by HPV16/18 decreases with age at diagnosis. Impact: The absolute risk of an invasive cancer due to non-HPV16/18 in women under 35 is extremely low. This finding might prompt us to rise the age at which public HPV screening for vaccinated women should start. Cancer Epidemiol Biomarkers Prev; 19(9); 2389-400. ©2010 AACR.

Expression of human papillomavirus E6 and E7 oncoprotein mRNA in women with low-grade squamous intraepithelial lesions or less

“…12.1% of cases had ASC-US and 10.9% had a negative cytology. Such findings agree with the results from studies by Gargiulo et al (2007), Brismar-Wendel et al (2009), Carozzi et al (2010, and Correnti et al (2011).…”

Age and Survival of Cervical Cancer Patients with Bone Metastasis

“…This is consistent with an international study which observed a decreasing proportion of HPV16/18-positive cancers with increasing age, (de Sanjose et al, 2013). In 10.2% of invasive cancers, in which coinfections were observed, HPV 16 was involved in the highest rate of coinfections along with other HPV types (77%), (Carozzi et al, 2010). Populations at high risk, such as early lifetime sexual contacts, multiple lifetime sexual partners, cigarette smoking habits, immunocompromised hosts and history of sexually transmitted diseases, have multiple high risk HPV type infections and develop cervical cancer at early age.…”

“…Out of 17 high-risk types among 110 types of HPV, HPV type 16 causes 50% and type 18 causes 20% of cervical cancers. The majority of the patients (89-92%) were aged under 45 ,who had HPV 16/18 infected tumors, which were more than the older patients (73-78%), (Carozzi et al, 2010). This is consistent with an international study which observed a decreasing proportion of HPV16/18-positive cancers with increasing age, (de Sanjose et al, 2013).…”

Expression of Messenger RNA of Oncoproteins E6 and E7 of Human Papilomavirus in Women with Negative Oncotic Cytologies, Epithelial Squamous Atypias of Undefined Significance and Low-Grade Intraepithelial Lesions

“…Correnti et al, 2011, also found a higher percentage of multiple infections in patients with low grade infections, comparing with patients with cervix carcinoma, said findings also agreeing with the studies by Gargiulo et al, 2007 andCarozzi et al, 2010 [21]- [23].…”