Prevalence of human papillomavirus infection and correlation with cervical lesions in Japanese women

Ishi, Kazuhisa; Suzuki, Fujihiko; Yamasaki, Shigetaka; Suto, Hiroko; Kina, Katsunari; Nojima, Masanori; Yoshida, Koyo

doi:10.1111/j.1447-0756.2004.00213.x

Cited by 9 publications

(4 citation statements)

References 18 publications

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“…Surprisingly, only 41% of HSIL samples showed single‐HPV type infections in the present study. In a previous Japanese study using a consensus primer‐based PCR assay and exfoliative cytology samples from CIN2‐3 cases, a single HPV type infection was found in 88.7% . In a study using GS‐31, 54% of CIN 2, 3 cases were shown to be infected with a single HPV type.…”

Section: Discussionmentioning

confidence: 56%

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Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types

Okodo

Okayama

Teruya

et al. 2018

Journal of Medical Virology

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We have developed a new human papillomavirus (HPV) assay using the uniplex E6/E7 polymerase chain reaction (PCR) method, which is able to detect E6 and E7 genes in 39 HPV types. We validated the assay for sensitivity and specificity using cloned HPV DNA and clinical samples. A comparative study using Genosearch-31 (GS-31) to determine HPV genotypes in clinical samples was also performed. E6 or E7 genes, measured by uniplex E6/E7 PCR, were detectable in 15 low-risk (HPV-6, -11, -40, -42, -44, -54, -55, -61, -62, -71, -74, -81, -84, -89, -90), 11 intermediate-risk (HPV-26, -30, -34, -53, -66, -67, -69, -70, -73, -82, -85), and 13 high-risk (HR) HPV types. The detection limit of this assay was 100 copies in all 39 HPV types and no cross-reactivity was observed with any type. This assay detected HPV in all 226 cervical cell samples, including 222 high-grade squamous intraepithelial lesions (HSIL) and 4 squamous cell carcinoma (SCC) cases, whereas GS-31 identified HPV in 99.6% (225/226) of the same samples. All SCC and 41.0% (90/222) of HSIL cases were infected with a single HPV type, while the remaining 59% of HSIL cases involved multiple HPV types. It was noted that high-risk and probably high-risk HPV types (HPV-66, -70 and -82) were identified, but no low-risk types were identified as a single-type infection in these HSIL and SCC cases. The uniplex E6/E7 PCR assay has high sensitivity, and can be useful tool in epidemiological studies or clinical follow-ups after surgery.

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Section: Discussionmentioning

confidence: 56%

“…Approximately 5‐15% of cervical cancer cases and a similar percentage of CIN3 were deemed HPV negative in previous studies . One possible explanation for these HPV‐negative cases is that an alternative TP53‐related pathway may induce malignant changes .…”

Section: Discussionmentioning

confidence: 97%

Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types

Okodo

Okayama

Teruya

et al. 2018

Journal of Medical Virology

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“…De SanJose reported the worldwide HPV prevalence via a meta‐analysis and found that the second peak of HPV prevalence in women after 44 years old presented with an area difference. For instance, in European and North American women, the HPV prevalence commonly declines after the age of 25 , while in Asian and Latin American women, the second rise in the HPV prevalence may emerge in some areas, such as China , Japan , Chile , Colombia , and Mexico . Some factors may be related to the second peak in the HPV prevalence, such as increased extramarital sexual behaviors of their husbands or themselves, or decreased immunity due to a female hormone decline during peri‐ or post‐menopause, which could lead to potential activation of HPV with a low replication status .…”

Section: Discussionmentioning

confidence: 99%

A cross‐sectional study on HPV testing with type 16/18 genotyping for cervical cancer screening in 11,064 Chinese women

Zhao

et al. 2017

Cancer Medicine

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Cytology‐based cervical cancer screening is restricted because of a lack of cytologists. Thus, HPV‐based instead of cytology‐based screening may be a more suitable strategy in China. Here, we assessed the effectiveness of HPV testing (Cobas® 4800 Test, Roche) and HPV‐based programs to detect high‐grade cervical intraepithelial neoplasia (CIN) or cancer compared with cytology (Thinprep, Hologic) and cytology‐based programs through a cross‐sectional study in 11,064 Chinese women aged 21–65 years who were enrolled from Longyou County in Zhejiang Province, China. The rates of HPV positivity and cytology abnormality were 9.8% and 6.1%, respectively. The HPV positivity rate had two age peaks, 21–24 (15.4%) and 60–65 (14.4%) years. According to adjusted data, HPV testing demonstrated significantly higher sensitivity and negative predictive value (NPV) than cytology for detecting CIN2 or worse (90.0% vs. 66.7%, 99.9% vs. 99.5%), and there was an acceptable specificity (91.3%) and positive predictive value (PPV, 12.5%). Furthermore, primary HPV testing with type 16/18 genotyping showed the highest sensitivity (78.6%) and NPV (99.7%) among four screening strategies, and there was similar specificity (96.8%) and PPV (23.9%) compared with co‐testing screening to detect CIN2+, while there were fewer colposcopies (4.2) and tests (106.3) performed than with co‐testing and primary cytology screening to detect a case of high‐grade CIN. The differences in effectiveness were approximately similar when CIN3+ was the identifying target. Our findings suggest that primary HPV testing with type 16/18 genotyping has a higher sensitivity and NPV, possesses optimal cost/effectiveness in the first round of screening and is a feasible strategy of cervical cancer screening for Chinese women.

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“…Over 90% of these conditions are HPV-positive; in some cases, the genome is only present in episomic or integrated form, in other cases, the complete virions are visible by electronic microscope [45,[60][61][62][63][64].…”

Section: Genital Potentially/overtly Malignant Lesions Hpv-relatedmentioning

confidence: 99%

Oral HPV Infection: Current Strategies for Prevention and Therapy

Syrjänen

Termine

Capra³

et al. 2012

CPD

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Infection with High Risk (HR) Human Papillomaviruses (HPVs) is the main aetiological agent of Cervical Squamous Cell Carcinoma (CSCC) and also associated in a subgroup of other neoplasms, including Oropharyngeal Squamous cell Carcinoma (OPSCC). HPV infection, in genital as in oral mucosa, can also be subclinical or associated with benign proliferative lesions (common warts, condylomas, papillomas) caused mostly by infection with Low Risk (LR)-HPVs. In the last decades, extensive research has resulted in growing knowledge on HPV biology and specifically viral life cycle, biochemical properties of viral proteins and their interaction with the host proteins leading to potential new targets of prophylactic or therapeutic vaccines and therapies for HPV infection. In addition, notable progresses have been made in the field of diagnostics to detect HPV DNA or RNA. The recent epidemiological data suggest the significant changes in HPV endemic, due to the changes in sexual habits especially among young generations (i.e. early sexual debuts, multiple sexual partners, oral and anal sex); this scenario has urged on the need of adequate campaigns of primary (sexual education, vaccination programs) and secondary prevention (diagnostics of HPV-related diseases). Due to the growing interest on HPV infection and HPV related cancers, the authors made a narrative review of the literature on oral HPV infection and oral-genital transmission. After this, in view of the controversies about the strategies of therapy and prevention of HPV infection, the present review focuses on the current state of art about the available tools for the therapeutic and, if any, preventive management of oral HPV infection.

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Prevalence of human papillomavirus infection and correlation with cervical lesions in Japanese women

Abstract: Diagnosis of HPV infection is also important for the prediction of progression to CIN and cervical cancer.

Cited by 9 publications

References 18 publications

Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types

Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types

A cross‐sectional study on HPV testing with type 16/18 genotyping for cervical cancer screening in 11,064 Chinese women

Oral HPV Infection: Current Strategies for Prevention and Therapy

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