Prevalence of human papillomavirus in oral gargles and tonsillar washings

Cho, H.; Kishikawa, Toshihiro; Tokita, Yuko; Suzuki, Motoyuki; Takemoto, Norihiko; Hanamoto, Atsushi; Fukusumi, Takahito; Yamamoto, Misa; Fujii, Makoto; Ohno, Yuko; Inohara, Hidenori

doi:10.1016/j.oraloncology.2020.104669

Cited by 12 publications

(18 citation statements)

References 19 publications

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“…The DNA of the specimens was amplified using PCR-rSSO (GENOSEARCH), and the type of HPV (HR HPV or low-risk HPV) was identified. The procedures for detecting oral HPV infection was detailed elsewhere (Cho et al 2020).…”

Section: Data Collectionmentioning

confidence: 99%

“…It is important to identify the influential factors and implications of HPV infection in Japan, because there is not enough knowledge regarding the current status of HR HPV infection or the associations between HPV infection and influential factors identified in other countries and there are insufficient measures being taken to prevent HPV infection. We have examined 437 Japanese medical professionals and found that the prevalence of oral HR HPV infection was 4.4% (Cho et al 2020). Therefore, we designed the present study to investigate the relationship between the oral HR HPV infection and sexual behavior in the same cohort.…”

Section: Introductionmentioning

confidence: 99%

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Exploring the relationship between oral high-risk HPV infection and sexual behavior among over 400 medical professionals in Japan

Tokita

Ohno

Cho

et al. 2020

J Public Health (Berl.)

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Objectives This study investigated the relationship between oral high-risk HPV (HR HPV) infection and sexual behavior in Japanese medical professionals. Materials and methods We collected oral specimens and a self-administered questionnaire from 234 men and 171 women. Results The oral HR HPV infection prevalence was 4.7% (95% confidence interval [CI]: 3.0-7.2]) overall, 7.3% (95% CI: 4.6-11.3) for men, and 1.2% (95% CI: 0.3-4.2) for women. The number of sex partners had a significant influence on this prevalence, with variation by type of sexual behavior. The prevalence of oral HR HPV infection was significantly higher (p < 0.0001) among those with more than 20 lifetime overall sex partners (23.8%, 95% CI: 13.5-38.5) or oral sex partners (25.0%, 95% CI: 14.2-40.2). In terms of the number of vaginal sex partners, the prevalence was approximately the same for those with 6-10 (8.3%, 95% CI: 3.9-17.0), 11-20 (11.1%, 95% CI: 5.2-22.2), or more than 20 (11.5%, 95% CI: 4.0-29.0) partners (p = 0.0043). Furthermore, dividing the number of vaginal and oral sex partners into four categories (both>5, only vaginal>5, only oral>5, and both≤5), the infection prevalence was significantly higher when both vaginal and oral sex partners were more than five (12.5%, 95% CI: 7.7-19.6). The prevalence was also higher for those who had more oral than vaginal partners, compared with other groups (13.5%, 95% CI: 6.7-25.3). Conclusions and clinical relevance Oral HR HPV infection in Japan seems to be influenced by sexual behavior, and preventive health efforts such as vaccination and health education should be implemented.

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Section: Data Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring the relationship between oral high-risk HPV infection and sexual behavior among over 400 medical professionals in Japan

Tokita

Ohno

Cho

et al. 2020

J Public Health (Berl.)

View full text Add to dashboard Cite

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“…Comparison of oral gargle and tonsillar washing (performed by a clinician using a specific device) was carried out by Choo et al [32] to investigate whether the addition of tonsillar washing to gargling could improve HPV detection. Once resuspended in PBS, exfoliated cells from both types of sample were used for nucleic acid extraction with a QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany).…”

Section: Sample Collection Nucleic Acid Extraction and Purificationmentioning

confidence: 99%

Human Papillomavirus Oral Infection: Review of Methodological Aspects and Epidemiology

et al. 2021

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Oral infection by Human Papillomavirus (HPV) has recently gained great attention because of its involvement in the development of a subset of head and neck squamous cell carcinoma. The role of specific Alpha-HPVs in this regard has been well established, whereas the contribution of other genera is under investigation. Despite their traditional classification as “cutaneous” types, Beta and Gamma HPVs are frequently detected in oral samples. Due to the lack of a standardized protocol, a large variety of methodologies have been used for oral sample collection, DNA extraction, HPV detection and genotyping. Laboratory procedures influence the evaluation of oral HPV prevalence, which largely varies also according to the population characteristics, e.g., age, gender, sexual behavior, Human Immunodeficiency Virus (HIV) status. Nevertheless, oral infection by Beta and Gamma HPVs seems to be even more common than Alpha-HPVs. The latter is 5–7% in the general population, and increases up to 30% approximately in HIV-infected men who have sex with men. Despite major advances in the evaluation of oral HPV prevalence, its natural history is still little understood, especially for Beta and Gamma HPVs. The latest technologies, such as Next Generation Sequencing (NGS), can be exploited to gain new insights into oral HPV, and to improve the identification of novel HPV types.

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“…Detection of HPV is performed using different methods, including signal amplification assays, assays based on hybridization, real-time PCR, microarrays, and next-generation sequencing [ 29 , 30 ]. The methodology used in our study detects 33 subtypes, including the non-oncogenic HPV 43/44, while other assays detect only oncogenic genotypes and fewer non-oncogenic genotypes, without HPV 43/44 [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. Some researchers have used tests to detect all known high-risk and low-risk genotypes.…”

Section: Discussionmentioning

confidence: 99%

“…Some researchers have used tests to detect all known high-risk and low-risk genotypes. However, HPV 43/44 was not observed [ 37 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. There are no guidelines for HPV testing as regards head and neck cancer, and the methods to detect HPV DNA in oral samples have not been standardized yet [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland

et al. 2021

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Human papillomavirus (HPV) is a virus with the potential to infect human epithelial cells and an etiological agent of many types of cancer, including head and neck cancer. The aim of the study was to determine the prevalence of HPV infection in patients with head and neck squamous cell carcinoma (HNSCC), patients with chronic tonsillitis, and healthy individuals, and to establish high- and low-risk HPV genotypes in these groups. The objectives also comprised the delineation of the relationship between the infection with high- or low-risk HPV subtypes and clinicopathological and demographic characteristics of the study groups. This study was composed of 76 patients diagnosed with HNSCC, 71 patients with chronic tonsillitis, and 168 cases without either of these conditions (the control group). HPV detection and identification of subtypes were performed on isolated DNA using a test which allowed detection of 33 common high-risk and low-risk HPV subtypes. The prevalence of HPV infection was 42.1%, 25.4%, and 37.5% in HNSCC, chronic tonsillitis, and control groups, respectively. HPV 16 was the most prevalent genotype in all groups and the non-oncogenic HPV 43/44 was frequent in HNSCC patients. This analysis provides insight into the prevalence of oral oncogenic and non-oncogenic HPVs in patients with head and neck cancer, patients with chronic tonsillitis and healthy individuals, and leads to the conclusion that further investigations are warranted to examine a larger cohort of patients focusing on high- and low-risk HPV genotypes. Efforts should be focused on screening and prevention strategies, and therefore, it is important to introduce tools for effective detection of HPV genotypes. Furthermore, given the role of vaccines against oral HPV infection, our observations lead to the suggestion that HPV vaccination should be of considerable importance in public health strategies.

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Prevalence of human papillomavirus in oral gargles and tonsillar washings

Cited by 12 publications

References 19 publications

Exploring the relationship between oral high-risk HPV infection and sexual behavior among over 400 medical professionals in Japan

Exploring the relationship between oral high-risk HPV infection and sexual behavior among over 400 medical professionals in Japan

Human Papillomavirus Oral Infection: Review of Methodological Aspects and Epidemiology

The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland

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