Prevalence of human papillomavirus (HPV) and HPV-16 genotyping by real-time PCR in patients with several cervical pathologies

Dinç, Bedia; Rota, Seyyal; Onan, A.; Bozdayı, Gülendam; Taşkiran, Ç.; Bırı, Aydan; Güner, Haldun

doi:10.1590/s1413-86702010000100005

Cited by 5 publications

(1 citation statement)

References 28 publications

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“…Polymerase chain reaction (PCR) based methods amplifying nucleic acids of HPV are commonly used because of the limited sensitivity of Pap smear test leading to sample preparation and interpretation problems, insufficient serological tests and impossibility of in vitro cultures. Detection of HPV DNA, especially in latent infections, can be helpful in detecting cancer and precursor lesions [4]. Real-time PCR techniques have been developed to quantify HPV DNA in clinical samples.…”

Section: Introductionmentioning

confidence: 99%

Screening for High-Risk Human Papillomavirus by Real-Time PCR and Its Association With Cytological Abnormalities of Pap Smears in Baghdad

DS¹,

Hy²,

FG³

2012

Int J of Micr Res

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Abstract-Human Papillomaviruses (HPVs) are known to be etiological agents of cervical cancer and have been found in 99.7% of women with high-grade (HG) cervical intraepithelial neoplasia (CIN) pre-cancer. Testing of high-risk HPV (HR-HPV) has been proposed as a way of improving cervical screening, especially for women with low-grade (LG) Papanicolaou (Pap) smears. Therefore, the present study aimed to investigate the diagnostic correlation of real-time PCR technique with Pap smear results for 13 genotypes of HR-HPV in 415 of suspected women. Pap smear results showed the suspected women with abnormal and normal cytological changes were 79.3% (329/415) and 20.7% (86/415), respectively. In contrast, real-time PCR results showed that women with or without abnormal changes had HR-HPV DNA positive were 50.5% (166/329) and 10.5% (9/86), respectively. Thus, Pap smear results had no sensitive to predicate risky for LG-CIN women were observed. According to the real-time PCR results, the incident of HG or cancer was more associated with HR-HPV. Our findings indicated the real-time PCR test with the cytological test as a routine practice for women with LG-CIN reduces the incidence of HG or cancer. After that, the prospective studies should be appropriated to determine HR-HPV genotypes and viral load (copies/cell) with realtime PCR test as predicate risk-factor.

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Section: Introductionmentioning

confidence: 99%

Screening for High-Risk Human Papillomavirus by Real-Time PCR and Its Association With Cytological Abnormalities of Pap Smears in Baghdad

DS¹,

Hy²,

FG³

2012

Int J of Micr Res

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Prevalence and genotype distribution of human papillomavirus in non‐neoplastic cervical tissue lesion: Cervical erosion

Bayram

Erkılıç

Balat

et al. 2011

Journal of Medical Virology

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Human papillomavirus (HPV) infection is the commonest sexually transmitted infection, which is associated with various clinical conditions, ranging from asymptomatic infection to malignant disease of the cervix. The aim of this study was to evaluate the prevalence and genotypic distribution of HPV in women with cervical erosion and to compare the results with those in women with a clinically normal cervix. A further aim was to establish the association between HPV infection and cervical cytology results in women with and without cervical erosion. Cervical samples were collected by liquid-based method and consecutively evaluated for the presence of HPV DNA and for cervical cytology. HPV DNA was tested by a nested polymerase chain reaction (PCR) and typed by reverse dot blot genotyping. Cytological classification was made according to Bethesda 2001 criteria. The overall HPV prevalence was 16.9%; HPV DNA was positive in 20.2% of women with cervical erosion and 12.8% in women with normal cervix (P < 0.05). Multiple infections were found in 34.1% of the HPV-positive women. Commonest types were HPV 18 (32.9%), HPV 16 (29.5%), HPV 54 (20.5%), and HPV 6 (17%). Cervical cytology results were abnormal for 5.2% of women with cervical erosion and for 1.3% with clinically normal cervix (P < 0.05). This study detected a high prevalence of HPV infection in women with cervical erosion compared to women with a normal cervix. This data may contribute to the HPV epidemiology in the southeastern Turkey. It is recommended that women with cervical erosion should be given priority in HPV screening programs.

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Prevalence of high-risk human papillomavirus by cobas 4800 HPV test in urban Peru

Iwasaki¹,

Galvez-Philpott²,

Arias‐Stella³

2014

The Brazilian Journal of Infectious Diseases

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Prevalence of human papillomavirus (HPV) and HPV-16 genotyping by real-time PCR in patients with several cervical pathologies

Cited by 5 publications

References 28 publications

Screening for High-Risk Human Papillomavirus by Real-Time PCR and Its Association With Cytological Abnormalities of Pap Smears in Baghdad

Screening for High-Risk Human Papillomavirus by Real-Time PCR and Its Association With Cytological Abnormalities of Pap Smears in Baghdad

Prevalence and genotype distribution of human papillomavirus in non‐neoplastic cervical tissue lesion: Cervical erosion

Prevalence of high-risk human papillomavirus by cobas 4800 HPV test in urban Peru

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