Prevalence of honeybee viruses in the Czech Republic and coinfections with other honeybee disease

Ryba, Štěpán; Titěra, Dalibor; Schodelbauerova-Traxmandlova, Iva; Kindlmann, Pavel

doi:10.2478/s11756-012-0038-5

Cited by 13 publications

(6 citation statements)

References 33 publications

(28 reference statements)

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“…Regarding viruses in general, SBV was the most prevalent one in our surveyed colonies (73.5%). This high prevalence of SBV is in line with other studies from European and Asian countries (64% France, [ 42 ]; 40% Croatia, [ 43 ]; 19% Belgium, [ 46 ]; 39% Japan, [ 22 ]; 21% China, [ 47 ]; 5% Czech Republic, [ 44 ]). BQCV was the second most prevalent virus in our colonies (49.7%).…”

Section: Discussionsupporting

confidence: 90%

“…BQCV was the second most prevalent virus in our colonies (49.7%). It has been reported with even higher frequencies in Japan and France (77% [ 22 ], and 58% [ 42 ], respectively) and with lower frequencies in China, Croatia, Czech Republic and Belgium [ 41 , 43 – 44 , 46 ]. Surprisingly, the prevalence of DWV (11.3%) was also low in comparison with other studies [ 22 , 41 – 45 ], despite the regular presence of the V .…”

Section: Discussionmentioning

confidence: 99%

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The Honey Bee Pathosphere of Mongolia: European Viruses in Central Asia

2016

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Parasites and pathogens are apparent key factors for the detrimental health of managed European honey bee subspecies, Apis mellifera. Apicultural trade is arguably the main factor for the almost global distribution of most honey bee diseases, thereby increasing chances for multiple infestations/infections of regions, apiaries, colonies and even individual bees. This imposes difficulties to evaluate the effects of pathogens in isolation, thereby creating demand to survey remote areas. Here, we conducted the first comprehensive survey for 14 honey bee pathogens in Mongolia (N = 3 regions, N = 9 locations, N = 151 colonies), where honey bee colonies depend on humans to overwinter. In Mongolia, honey bees, Apis spp., are not native and colonies of European A. mellifera subspecies have been introduced ~60 years ago. Despite the high detection power and large sample size across Mongolian regions with beekeeping, the mite Acarapis woodi, the bacteria Melissococcus plutonius and Paenibacillus larvae, the microsporidian Nosema apis, Acute bee paralysis virus, Kashmir bee virus, Israeli acute paralysis virus and Lake Sinai virus strain 2 were not detected, suggesting that they are either very rare or absent. The mite Varroa destructor, Nosema ceranae and four viruses (Sacbrood virus, Black queen cell virus, Deformed wing virus (DWV) and Chronic bee paralysis virus) were found with different prevalence. Despite the positive correlation between the prevalence of V. destructor mites and DWV, some areas had only mites, but not DWV, which is most likely due to the exceptional isolation of apiaries (up to 600 km). Phylogenetic analyses of the detected viruses reveal their clustering and European origin, thereby supporting the role of trade for pathogen spread and the isolation of Mongolia from South-Asian countries. In conclusion, this survey reveals the distinctive honey bee pathosphere of Mongolia, which offers opportunities for exciting future research.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

The Honey Bee Pathosphere of Mongolia: European Viruses in Central Asia

2016

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“…We report the first survey of the occurrence of Nosema ceranae and BQCV in Africanized honey bees from southern Brazil. Our findings indicate that these two agents, that have been implicated in population declines of these insects in several countries, are widespread in populations of Africanized honey bees in southern Brazil (Ryba et al 2012, Mendoza et al 2013. However, the occurrence and infection profiles of N. ceranae and BQCV differs significantly across different counties in southern Brazil.…”

Section: Discussionsupporting

confidence: 51%

Black queen cell virus and Nosema ceranae coinfection in Africanized honey bees from southern Brazil

et al. 2020

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Bees are fundamental in several aspects, especially in relation to plant biodiversity and pollination. Recently, immense losses are being faced in the number of Brazilian colonies, mainly in southern states of the country, which has a strong beekeeping activity. There are indications that, among the reasons for the losses, pathogens that affect the health of bees may be involved. Among them, the microsporidium Nosema and the black queen cell virus (BQCV) stand out for their prevalence. In this study, 92 colonies of 17 apiaries from southern Brazil were evaluated for infection by Nosema ceranae, Nosema apis and BQCV. Nucleic acid extractions and cDNA synthesis were performed from adult bee samples, followed by Reverse Transcription Polymerase Chain Reaction (RT-PCR) and multiplex PCR. Eight BQCV positive samples were subjected to sequencing. The results showed that N. ceranae and BQCV are circulating in the Southern region of the country, which may be the reason for the loss of colonies. N. apis was not found. N. ceranae was found in 57.6% (53/92) of the colonies and BQCV in 32.6% (30/92). Co-infection was found in 25% (23/92) of the colonies studied, a factor that is suggested to be reducing the hosts’ longevity due to the synergistic action of the pathogens. The samples submitted to sequencing indicated similarity of 96.8 to 100% between them, in addition to strong similarity with sequences from Asia, United States, Germany and Peru. This study reports the circulation of N. ceranae and BQCV in apiaries in southern Brazil, in addition to being the first phylogenetic analysis of the Brazilian BQCV sequence.

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“…Multiple infections are noted in other reports with values reaching 92 % of the colonies screened (Tentcheva et al 2004;Antùnez et al 2006;Forgach et al 2008;Ai et al 2012;Chen et al 2004;Ryba et al 2012). Their effect on honey bee health is yet to be determined.…”

Section: Discussionmentioning

confidence: 93%

Atypical viral and parasitic pattern in Algerian honey bee subspecies Apis mellifera intermissa and A. m. sahariensis

et al. 2015

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-Unusually high losses of honey bee colonies are reported in many regions of the world, but little data is available concerning the status of honey bee stocks in Africa. However, the situation on this continent, where beekeeping is weakly developed and where the wild population of the pollinator remains large, can give us an insight on the causes of increased mortalities elsewhere. In this study, we evaluate the health status of Apis mellifera intermissa and A. m. sahariensis populations inhabiting the north and the south of Algeria, respectively. We report few colony losses associated with an atypical pattern of prevalence for common honey bee parasites and pathogens. The presence or absence of these risk factors is discussed in relation to the occurrence of local and global colony losses to contribute to our understanding of how honey bee pathogens and parasite impact this pollinator's health.Apis mellifera intermissa / Apis mellifera sahariensis / virus / Nosema spp. / Varroa destructor / colony losses / Algeria

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Prevalence of honeybee viruses in the Czech Republic and coinfections with other honeybee disease

Cited by 13 publications

References 33 publications

The Honey Bee Pathosphere of Mongolia: European Viruses in Central Asia

The Honey Bee Pathosphere of Mongolia: European Viruses in Central Asia

Black queen cell virus and Nosema ceranae coinfection in Africanized honey bees from southern Brazil

Atypical viral and parasitic pattern in Algerian honey bee subspecies Apis mellifera intermissa and A. m. sahariensis

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