Prevalence of hepatitis E virus among swine and humans in two different ethnic communities in Indonesia

Utsumi, Takako; Hayashi, Yoshitake; Lusida, Maria Lucia Inge; Amin, Mochamad; Soetjipto,; Hendra, Agus; Soetjiningsih,; Yano, Yoshihiko; Hotta, Hak

doi:10.1007/s00705-010-0883-x

Cited by 18 publications

(13 citation statements)

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“…After combining the Yogyakarta and Tulungagung samples as all from Java, the prevalence of anti‐HEV antibody seropositivity in 1‐ and 2‐month‐old swine was found to be 5.2% (1/19) and 43.3% (13/30), respectively. Eleven of 16 (68.8%) 1‐month‐old swine and 18/20 (90%) 2‐month‐old swine in Bali were positive for anti‐HEV antibodies . There were significant differences between Java and Bali in the rates of anti‐HEV antibody seropositivity, seropositivity in the 1‐ and 2‐month‐old swine being higher in Bali than in Java ( P < 0.001 and P =0.01).…”

Section: Resultsmentioning

confidence: 93%

“…The total prevalence of anti‐HEV in humans in Bali was 11.6%. Of 64 workers and 135 local residents from Bali, 12 (18.8%) and 11 (8.1%), respectively, were anti‐HEV antibody positive . The overall prevalence of anti‐HEV antibody seropositivity in Bali was significantly higher than that in Java ( P = 0.015).…”

Section: Resultsmentioning

confidence: 93%

“…In addition, 73 swine serum samples and randomly‐chosen fecal samples from 27 of those swine were also obtained. This material was to complement our previous data obtained from serum of 12 and 64 swine farm workers and 42 and 135 local residents of Tulungagung (East Java) and Mengwi (Bali), respectively, plus serum samples from 89 swine in Tulungagung (East Java) and 119 swine in Mengwi (Bali) . (Table , Fig.…”

Section: Methodsmentioning

confidence: 96%

“…). In our previous study, we tested HEV status in swine farm workers from two different ethnic communities, albeit with a limited number of samples . This study represents our ongoing investigation of the prevalence of HEV infection in swine and humans from multiple areas in Java and Bali, Indonesia.…”

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confidence: 99%

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Hepatitis E virus infection in two different regions of Indonesia with identification of swine HEV genotype 3

Widasari

Yano

Utsumi

et al. 2013

Microbiology and Immunology

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Hepatitis E is an emerging disease with a high incidence globally. Few data are available on hepatitis E virus (HEV) infection in Indonesia. To obtain molecular information on HEV infection in two regions of Indonesia with different customs and swine breeding conditions, serum samples from 137 swine farm workers, 100 blood donors and 100 swine (27 fecal samples also obtained) in Yogyakarta (Central Java) and from 12 and 64 swine farm workers, 42 and 135 local residents and 89 and 119 swine in Tulungagung (East Java) and Mengwi (Bali), respectively, from our previous study, were compared. Serological tests for anti-HEV antibodies by ELISA, HEV-RNA detection by RT-PCR and phylogenetic analysis were performed. The total prevalence of anti-HEV antibodies in humans was higher in Bali (11.6%) than in Java (5.1%; P ¼ 0.015). No significant differences in anti-HEV prevalence among swine farm workers and local residents in Java were found. The finding of swine HEV genotype 3 in specimens from Yogyakarta and genotype 4 from Tulungagung and Bali is somewhat different from other reports. We suggest other factors in addition to close contact with swine might play an important role in HEV transmission of non-endemic/related custom groups. To the best of our knowledge, this is the first report on swine HEV genotype 3 in Indonesia.Key words hepatitis E virus, Indonesia, zoonosis transmission.Hepatitis E virus was first identified in 1983 in a study of human volunteers aimed at pinpointing the causative agent of a large, acute, icteric, self-limiting, enteric, non-A, non-B, hepatitis infection outbreak in India (1). A non-enveloped virus particle of approximately 27 nm in diameter (range, 27-34 nm) was visualized by immune electron microscopy of a mixture of acute phase stool and convalescent serum from a patient in that study. This virus was successfully inoculated into a Cynomolgus macaque and was cloned in 1990 (2). HEV is now classified in the genus Hepevirus and is the only member of the Hepeviridae family. It has an approximately 7.2 kb long, polyadenylated, single-stranded RNA genome, with short 5 0 and 3 0 untranslated regions, and three ORFs (3). Genomic sequence analysis has classified HEV isolates from humans and other mammals into four genotypes with at least 24 subgenotypes (1a-1e, 2a-2b, 3a-3j, and 4a-4g) (4). Each HEV genotype appears to have a distinct and, in some cases, overlapping geographical distribution. Regions with low standards of sanitation, which promotes viral transmission, have the highest rates of HEV infection. Studies have shown that HEV epidemics

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 96%

mentioning

confidence: 99%

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Hepatitis E virus infection in two different regions of Indonesia with identification of swine HEV genotype 3

Widasari

Yano

Utsumi

et al. 2013

Microbiology and Immunology

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“…As shown in Figure , a total of 1987 studies were initially found in CNKI, PubMed and OVID, in which 234 studies were potentially related to our study objectives and had a full text retrieved. Subsequently, 204 studies were excluded with reasons listed in Figure , and two studies were added in an updated search, yielding 32 studies for the meta‐analysis (Bouwknegt et al, ; Caruso et al, ; De Sabato et al, ; Drobeniuc et al, ; Engle, Yu, Emerson, Meng, & Purcell, ; Galiana, Fernandez‐Barredo, Garcia, Gomez, & Perez‐Gracia, ; Hinjoy et al, ; Hongwei, ; Jiang, Zheng, & Shijuan, ; Kang et al, ; Krumbholz et al, ; Lange et al, ; Lee et al, ; Liang et al, ; Liu et al, ; Love, Bjornsdottir, Olafsson, & Bjornsson, ; Lu et al, ; Masia et al, ; Meng et al, ; Nong, Li, & Yi, ; Olsen, Axelsson‐Olsson, Thelin, & Weiland, ; Silva et al, ; Traore et al, ; Utsumi et al, ; Vivek & Kang, ; Wu, Liao, Wang, Lou, & Wenzhong, ; Wu, Xie, et al, ; Yan et al, ; Yu et al, ; Zheng et al, ). Of the included studies, 24 were published in English and eight in Chinese.…”

Section: Resultsmentioning

confidence: 99%

Hepatitis E virus infection in swine workers: A meta‐analysis

Huang

Wagner

et al. 2018

Zoonoses and Public Health

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Hepatitis E virus (HEV) infects both humans and animals. Swine has been confirmed to be the principal natural reservoir, which raises a concern that HEV infection would be substantially increasing among swine workers. The present study calculated the pooled prevalence of IgG antibodies against HEV among swine workers and the general population in previous cross‐sectional studies. We conducted a meta‐analysis comparing the prevalence of HEV infection between swine workers and the general population, including local residents, blood donors and non‐swine workers. Through searches in three databases (PubMed and OVID in English, and CNKI in Chinese) and after study selection, a total of 32 studies from 16 countries (from 1999 through 2018) were included in the meta‐analysis. A random‐effect model was employed in the study; an I 2 statistic assessed heterogeneity, and the Egger's test detected publication bias. The comparative prevalence of anti‐HEV IgG was pooled from the studies. Compared to the general population, the prevalence ratio (PR) for swine workers was estimated to be 1.52 (95% CI 1.38–1.76) with the I 2 being 71%. No publication bias was detected (p = 0.40). A subgroup analysis further indicated increased prevalence of anti‐HEV IgG in the swine workers in Asia (PR = 1.49, 95% CI: 1.35–1.64), in Europe (PR = 1.93, 95% CI: 1.49–2.50) and in all five swine‐related occupations, including swine farmers, butchers, meat processors, pork retailers and veterinarians (PR ranged between 1.19 and 1.75). In summary, swine workers have a relatively higher prevalence of past HEV infection, and this finding is true across swine‐related occupations, which confirms zoonotic transmission between swine and swine workers.

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Diseases of the Liver

2017

Veterinary Medicine

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Prevalence of hepatitis E virus among swine and humans in two different ethnic communities in Indonesia

Cited by 18 publications

References 18 publications

Hepatitis E virus infection in two different regions of Indonesia with identification of swine HEV genotype 3

Hepatitis E virus infection in two different regions of Indonesia with identification of swine HEV genotype 3

Hepatitis E virus infection in swine workers: A meta‐analysis

Diseases of the Liver

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