HCV-3a isolates and sequence homology of more than 85% for all isolates [ Figure 1]. Similarly, the remaining three HCV-3b isolates from Uttarakhand also clustered with Canadian HCV-3b isolates with more than 86% identity at nucleotide level for all isolates [ Figure 1]. The Uttarakhand HCV isolates when compared with each other were found to have more sequence similarities except one isolate (HCV-3a/UKD-2/India) which showed lower sequence identity with other HCV 3a isolates.In our study, out of 33 patients who were HCV positive by PCR assay, 28 (84.8%) were from Udham Singh Nagar (USN) district and the remaining 5 (15.2%) were from the Nainital District. In our previous study from this region on blood donors, signifi cantly higher HCV seropositivity was seen among the Sikh population from the USN district. [5] USN is largely inhabited by the Sikh community, where many people are found to be associated in some form of working or visiting Canada. It is hypothesised that this could be the reason for the close association of Indian and Canadian HCV isolates. However, a larger cohort from this geographical region is required to test the hypothesis that this genotype may have spread from Canada to USN place via population migration and admixture.