Prevalence of Helicobacter Pylori in Asymptomatic Population a Pilot Serological Study in Bangladesh

Ahmad, Mubashir; Rahman, Mahbubur; Rumi, Amelia; Islam, Saiful; Huq, Farida; Chowdhury, M F; Jinnah, Fatema; Morshed, Muhammad; Hassan, Moustapha; Khan, Abul Kalam Azad; Hasan, Mahmud

doi:10.2188/jea.7.251

Cited by 77 publications

(77 citation statements)

References 20 publications

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“…Metronidazole resistance in H. pylori has been shown to be due to mutation in rdxA; mutation in frxA has also been shown to be associated with metronidazole resistance (11,12,23). In Bangladesh, the prevalences of H. pylori infection among infants, children, and adults are 61, 84, and 92%, respectively (1,21,22); however, information on antimicrobial susceptibility to commonly used drugs in H. pylori treatment is lacking. This study was conducted to evaluate (i) the prevalence of primary antibiotic resistance to commonly used antimicrobial agents and (ii) the genetic basis for metronidazole resistance in H. pylori isolates from Bangladesh.…”

Section: Eradication Ofmentioning

confidence: 99%

Antimicrobial Susceptibility of Helicobacter pylori Strains Isolated in Bangladesh

et al. 2004

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Antimicrobial susceptibility of 120 Helicobacter pylori isolates to metronidazole, tetracycline, clarithromycin, and amoxicillin was determined, and 77.5, 15, 10, and 6.6% of the isolates, respectively, were resistant. Only rdxA inactivation and both rdxA and frxA inactivation were responsible for metronidazole resistance in 66% (8 of 12) and 33% (4 of 12) of the isolates, respectively. Eradication ofHelicobacter pylori infection by treatment with two antimicrobial agents (clarithromycin and amoxicillin or metronidazole) and a proton pump inhibitor is recommended by various consensus groups (10,16,20). Antimicrobial resistance in H. pylori is a growing problem as it is the most important factor in determining treatment outcome. The prevalence of antimicrobial resistance varies with geographical regions (3, 25). Metronidazole resistance in H. pylori has been shown to be due to mutation in rdxA; mutation in frxA has also been shown to be associated with metronidazole resistance (11,12,23). In Bangladesh, the prevalences of H. pylori infection among infants, children, and adults are 61, 84, and 92%, respectively (1, 21, 22); however, information on antimicrobial susceptibility to commonly used drugs in H. pylori treatment is lacking. This study was conducted to evaluate (i) the prevalence of primary antibiotic resistance to commonly used antimicrobial agents and (ii) the genetic basis for metronidazole resistance in H. pylori isolates from Bangladesh.Consecutive patients attending the Gastroenterology Department of Dhaka Medical College Hospital for upper gastrointestinal endoscopy were enrolled during 1999 to 2001. Diagnosis of peptic ulcer (PU) and non-ulcer dyspepsia (NUD) or gastritis was based on endoscopic examination of the stomach and duodenum. Biopsy samples were taken from each patient for culture.Bacteria were grown in brain heart infusion agar with 7% sheep blood and incubated at 37°C in 5% O 2 , 10% CO 2 , and 85% N 2 for 3 to 6 days. The MICs of amoxicillin, clarithromycin, metronidazole, and tetracycline for the isolates were determined by the agar dilution method as described elsewhere (18,19). All tests were repeated twice, and H. pylori 26695 was used as a control. ␤-Lactamase production was tested by the chromogenic cephalosporin method (6). The molecular mechanism of susceptibility and resistance to metronidazole was studied in 12 isolates. Metronidazole-susceptible (Mtz s ) isolates were further studied (by inactivation of rdxA alone or rdxA and frxA for conversion into an Mtz r phenotype) by transformation of Mtz s isolates with plasmids pBS-rdxA-cam (rdxA::cat) and pBS-frxA-kan (frxA::kan) as described earlier (11,12).A total of 278 consecutive patients between 15 and 78 years of age were enrolled, and among them, 72.7% (202 patients) were male and 27.3% (76 patients) were female. Among the patients, 162 had PU and 116 had NUD and 62.6% (174 of 278) were culture positive for H. pylori. Among the culturepositive patients, 121 (69.5%) were male and 53 (30.4%) were female and 112 (64.3%) had PU ...

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Section: Eradication Ofmentioning

confidence: 99%

Antimicrobial Susceptibility of Helicobacter pylori Strains Isolated in Bangladesh

et al. 2004

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“…In most patients, H. pylori infection remains asymptomatic and only 10-20% of infected patients develop various gastro-duodenal pathologies including chronic gastritis, peptic ulcer disease, primary B-cell gastric lymphoma, and adenocarcinoma of the stomach 2,3 . Prevalence of H. pylori is very high in Bangladesh, about 92% of adult have been found to be sero-positive for H. pylori 4 . In Bangladesh, prevalence of duodenal and gastric ulcer is 11.98% and 3.58% respectively among population aged 15 years and above 5 .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Sensitivity Pattern of Helicobacter pylori Isolates among Subgroup of Bangladeshi Patients

Islam¹,

Nahar

Sarker³

et al. 2014

Faridpur Med Coll J

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:This cross sectional study was carried out at Bangabandhu Sheikh Mujib Medical University (BSMMU) and International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B) from July 2008 to September 2009. Aim of the study was to find out the antimicrobial susceptibility profile of Helicobacter pylori isolates from dyspeptic patients. Total 224 dyspeptic patients from Out Patient Department (OPD) of BSMMU were initially enrolled after informed written consent. After upper GI endoscopy 157 patients were finally included who had erosions, ulcers or atrophic changes in the stomach or duodenum. Two biopsy samples were taken from each of them. Samples were incubated at 37 0 C in a double gas incubator with 5%O 2 , 10%CO 2 and 85%N 2 . Total 82 (52.23%) samples were found positive for H. pylori. Isolated organisms were then tested for sensitivity to Amoxicillin, Clarithromycin, Tetracycline, Levofloxacin and Metronidazole by Agar dilution method. Among 82 patients 51(62.2%) were male and 31(37.8) were female with a male:female ratio 1.6:1. Patients were categorized into two groups one having gastric or duodenal ulcer (30.5%) and other having no ulcer (69.5%). Among these isolates 92.7% were sensitive to Amoxicillin, 89% to Clarithromycin, 81.7% to Tetracycline, 80.5% to Levofloxacin and only 26.8% to Metronidazole. Beside these, 81.7% isolates were sensitive to both Amoxicillin and Clarithromycin, 74.4% to Amoxicillin and Tetracycline, 73.2% to Amoxicillin and Levofloxacin, 72% to Clarithromycin and Tetracycline, 59% to Clarithromycin and Levofloxacin and 51% to Tetracycline and Levofloxacin.

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“…Probably, IgG was positive in those cases due to past infection or subclinical exposure to H. pylori. In contrast to serum IgG, the IgA titers rise rapidly after infection and decrease if the infection is cleared [2,3].…”

Section: Rapid Urease Test (Rut)mentioning

confidence: 96%

“…Like other developing countries, the prevalence of H. pylori is very high in Bangladesh. The reported prevalence of H. pylori infection in adults is about 90% and more than 80% children become infected with H. pylori by the age of 6-9 years [2,3]. Both invasive and non-invasive tests are available for the diagnosis of H. pylori infection.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Rapid stool antigen test for the diagnosis of Helicobacter pylori infection in patients with dyspepsia

et al. 2017

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Prevalence of Helicobacter Pylori in Asymptomatic Population a Pilot Serological Study in Bangladesh

Cited by 77 publications

References 20 publications

Antimicrobial Susceptibility of Helicobacter pylori Strains Isolated in Bangladesh

Antimicrobial Susceptibility of Helicobacter pylori Strains Isolated in Bangladesh

Antimicrobial Sensitivity Pattern of Helicobacter pylori Isolates among Subgroup of Bangladeshi Patients

Evaluation of Rapid stool antigen test for the diagnosis of Helicobacter pylori infection in patients with dyspepsia

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