Prevalence of Helicobacter-like organisms in porcine gastric mucosa: A study of swine slaughtered in Italy

Grasso, G.; Ripabelli, Giancarlo; Sammarco, Michela Lucia; Ruberto, Addolorato; Iannitto, Giorgio

doi:10.1016/0147-9571(96)00007-0

Cited by 45 publications

(45 citation statements)

References 17 publications

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“…Authors claimed that contact with sheep and sheepdogs was a risk factor for infection [16]. However, other authors failed to isolate H. pylori and/or to detect its antibodies due to natural infection from stray and pet cats [42,43] and pigs in abattoirs [44]. These questionable data suggest doubtful zoonotic transmission of such agent, and whether animals are true reservoir hosts for H. pylori or not is still not obvious.…”

Section: Discussionmentioning

confidence: 90%

Serologic evidence and risk factors for Helicobacter pylori infection in animals and humans

Elhariri

Elhelw

Hamza

et al. 2017

J Infect Dev Ctries

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Introduction: Helicobacter pylori (H. pylori) is one of the most common bacterial infections among humans worldwide. Although many records imply its interfamilial acquisition, the role of animals remains poorly understood. This study was undertaken to investigate the seroprevalence of H. pylori in animals and their human contacts in Cairo and Giza governorates, Egypt. Methodology: Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to detect IgG antibodies to H. pylori in dogs, cattle, and humans. Results: Seropositive dogs (35/94; 37.2%), cattle (24/80; 30%) and humans (40/90; 44.4%) were found. Seroprevalence in animals significantly varied in different areas of sample collection, but there was no association with sex or age. Human seropositivity rates were associated with increasing age; moreover, seropositive dog owners (51.7%; 15/29), had seropositive dogs. However, infection was not associated with subject's sex, occupation, or history of animal contact. Conclusions: Our findings indicate H. pylori is widely distributed in cattle and dogs and their human contacts in Cairo and Giza, Egypt. Further studies to determine infection in other occupational groups are needed. This study provides baseline information on the seroprevalence of H. pylori, which may be required to begin prevention control programs in our area.

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Section: Discussionmentioning

confidence: 90%

Serologic evidence and risk factors for Helicobacter pylori infection in animals and humans

Elhariri

Elhelw

Hamza

et al. 2017

J Infect Dev Ctries

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“…Some studies have found an association between these two events (Barbosa et al, 1995;Queiroz et al, 1996;Roosendaal et al, 2000;Choi et al, 2001), while others have not (Grasso et al, 1996;Krakowka et al, 1998;Melnichouk et al, 1999). Anyway, even if the presence of the microorganism is not strictly related to the ulcer development, it can play a role in the multifactorial aetiology of the disease (Szeredi et al, 2005).…”

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confidence: 99%

Detection of Helicobacter candidatus suis by pcr in oesophagogastric ulcers of swine in Italy

Appino

Guarda

Pregel

et al. 2006

Acta Veterinaria Hungarica

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The aim of this study was to evaluate by PCR the presence of Helicobacter spp. in gastric mucus from the fundic region of the stomach and to investigate its role in oesophagogastric ulcers in swine bred and regularly slaughtered in Piedmont (Northern Italy). Stomachs from 595 regularly slaughtered swine were subjected to gross pathological examination in order to evaluate the presence of gastric ulcers (revealed in 75 cases, 12.6%). Histopathological examination was performed to better characterise erosions and ulcers. DNA extracted from gastric mucus collected from all the ulcer-affected and from 25 normal stomachs was submitted to PCR using Helicobacter spp. 16S rRNA gene target primers. Sixtythree percent (47/75) of the affected stomachs was positive as well as 24% (6/25) of the non-affected ones. Sequence analysis from 5 positive samples showed 99% homology with Helicobacter candidatus suis 16S ribosomal RNA gene.Key words: Swine, gastric ulcer, Helicobacter, PCR Gastric ulcer in swine is characterised by an area of peptic digestion usually localised in the pars oesophagea of the stomach. Its aetiology is uncertain. The changes developing in the gastric mucosa result in a decrease of body weight with economic losses for the farmer. The presence of the ulcer is often revealed at the slaughterhouse and, although it has been diagnosed with increasing frequency since 1950, little is known about the pathogenesis. Some authors suggest that it is probably related to the fast growth rate (Preziosi et al., 2000).

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“…Sci. 62 (7): [725][726][727][728][729] 2000 "Gastrospirillum suis", a tightly spiral bacterium, colonizes the gastric mucosa of swine [1,4,7,8]. By 16S rRNA sequencing, the bacterium is known to belong to the genus Helicobacter and is 99.5% similar to "G. hominis" type 1 [3,6].…”

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confidence: 99%

Association of Tightly Spiraled Bacterial Infectrion and Gastritis in Pigs.

Park

Lee

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. Tightly spiral bacteria were observed only in the pyloric mucosa of 4 (8.0%) of 50 swine stomachs, mainly in the surface of epithelia, the gastric pits and the lumen of gastric glands. The presence of the spiral bacteria was significantly associated with chronic pyloric gastritis (p<0.05). Mean gastritis score of the bacteria-positive pyloric mucosa was 3.25 ± 0.25, whereas that of the bacterianegative pyloric mucosa was 2.37 ± 0.12. Parakeratosis and hyperkeratosis were spontaneously seen in the mucosa layer of pars oesophagea, regadless of the bacterial infection. Marked infiltration of mononuclear cells and granulocytes were seen in the cardiac mucosa, regardless of the bacterial infection. Mean gastritis score of the bacteria-positive cardiac mucosa was 3.27 ± 0.32, whereas that of the bacteria-negative cardiac mucosa was 2.84 ± 0.13. There was no significant difference between the bacteria-positive and negative cardiac mucosa (p>0.05). Inflammatory response in the fundic mucosa was rare (gastritis score=0.75 ± 0.08). The tightly spiraled bactera were not cultured with various culture media. These results suggest that the presence of tightly spiraled bacteria is associated with only the pyloric gastritis in pigs.KEY WORDS: chronic gastritis, gastric mucosa, swine, tightly spiraled bacteria.J. Vet. Med. Sci. 62 (7): [725][726][727][728][729] 2000 "Gastrospirillum suis", a tightly spiral bacterium, colonizes the gastric mucosa of swine [1,4,7,8]. By 16S rRNA sequencing, the bacterium is known to belong to the genus Helicobacter and is 99.5% similar to "G. hominis" type 1 [3,6]. A possible link between such bacteria and gastric diseases of pig has been evoked [1,12]. Such diseases contribute an important problem in veterinary medicine leading to significant economic consequences, with up to 2.5% of mortality in pigs due to gastrointestinal hemorrhage [14]. Ulceration of the gastric pars esophagus is also a common problem in intensive pig production [1,12]. Several factors have been suspected in the pathogenesis of these lesions, but the etiology still remains unknown.In the present report, we investigated naturally occurring swine gastritis in Korea. In addition, the gastritis scores in the cardiac, fundic, and pyloric mucosa of swine stomach were calculated and analyzed for an association of the gastritis with the tightly spiraled bacterial infection. MATERIALS AND METHODSSample preparation: Stomach samples were obtained from 50 apparently healthy pigs (about 5 months old) weighing about 100-110 kg after slaughter at a slaughterhouse in Suwon, Korea. The stomach samples were transported to the laboratory in individual ice box (4°C) and cut open along the greater curvature from the pylorus to the diverticulum, and the contents were discarded. The stomachs were then washed gently in tap water, taking care to remove food particles only. The mucosal surfaces were examined and assessed for macroscopic lesions.Histopathological and microbiological examination: Fragments of the pars esophagea, cardia, fu...

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Prevalence of Helicobacter-like organisms in porcine gastric mucosa: A study of swine slaughtered in Italy

Cited by 45 publications

References 17 publications

Serologic evidence and risk factors for Helicobacter pylori infection in animals and humans

Serologic evidence and risk factors for Helicobacter pylori infection in animals and humans

Detection of Helicobacter candidatus suis by pcr in oesophagogastric ulcers of swine in Italy

Association of Tightly Spiraled Bacterial Infectrion and Gastritis in Pigs.

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