Serologic evidence and risk factors for Helicobacter pylori infection in animals and humans

Elhariri, Mahmoud; Elhelw, Rehab; Hamza, Dalia; El-Mahallawy, Heba S.

doi:10.3855/jidc.9339

Cited by 14 publications

(15 citation statements)

References 28 publications

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“…The prevalence of H . pylori was 29.4%, which is similar to that reported in other studies (19, 21, 27%) 6 , 33 , 34 . Comparing the occurrence of H .…”

Section: Discussionsupporting

confidence: 90%

“…We demonstrated recently a high sero-prevalence of H . pylori in humans having contact with animals 6 . Additionally, other studies reported that people that have direct contact with farm animals such as abattoir workers 30 and shepherds 31 , 32 have a high prevalence of H .…”

Section: Discussionmentioning

confidence: 99%

“…Our findings are in accordance with other studies that showed high prevalence of H . pylori in humans having contact with animals 6 , 32 , 33 and their family members 31 .…”

Section: Discussionmentioning

confidence: 99%

“…In Egypt, studies have shown a high prevalence of H . pylori infection among apparently healthy adult individuals 4 – 6 , school children 7 , and newborns 8 . The majority of colonized patients do not show any symptoms, while long-term carriage of this pathogen significantly increases the risk of developing gastric cancer 9 , 10 .…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of cagA+ vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk

Elhariri

Hamza

Elhelw

et al. 2018

Sci Rep

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Cases of human gastric cancer due to Helicobacter pylori have been reported worldwide and animals might act as a reservoir of infection in certain circumstances. The recent few decades showed a rapid decline in the incidence of gastric cancer, which was mainly due to the decrease in H. pylori infection. The aims of the present study were to determine the prevalence of H. pylori among livestock and investigate whether the animal isolates can be transmitted through contaminated milk causing gastric infection. Feces and milk samples were collected from apparently healthy cows, buffaloes, and sheep, and were examined by nested PCR and genotyping. The PCR positive samples were further subjected to bacterial culture followed by partial 16s sequencing of the isolates. Twenty-nine percent of the animals showed the presence of H. pylori, mainly the virulent cagA+vacA+s1a m1 i1 genotype, which is known to be associated with serious diseases in humans. The spiral viable culturable form (SVCF) of this strain was inoculated into UHT (ultra-high temperature) milk and remained viable for up to 10 days at 4 °C. Increasing period of storage and or temperature led to a decrease in the number of the SVCF and occurrence of the coccoid viable non-culturable form (CVNCF). The infectivity of the survived forms was determined by feeding healthy groups of laboratory mice with the contaminated UHT milk containing SVCF or CVNCF for 40 days. The gastric mucosa of the two mice groups showed similar levels of H. pylori load. This highlights that H. pylori can persist in contaminated milk by entering a non-culturable state, which can induce gastric infection.

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“…The prevalence of H . pylori was 29.4%, which is similar to that reported in other studies (19, 21, 27%) 6 , 33 , 34 . Comparing the occurrence of H .…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

“…Our findings are in accordance with other studies that showed high prevalence of H . pylori in humans having contact with animals 6 , 32 , 33 and their family members 31 .…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Occurrence of cagA+ vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk

Elhariri

Hamza

Elhelw

et al. 2018

Sci Rep

Self Cite

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“…The prevalence of helicobacter was investigated in 290 patients from Macau, China [40]. In Egypt, serological detection of H. pylori was done by [27] in dog and human revealed that 37.2% (35/94), 44.4% (40/90) respectively was positive. Twenty -nine serum samples which taken from owners of infected dog revealed 51.7% were found to have antibodies to H. pylori.…”

Section: Discussionmentioning

confidence: 99%

Dog as Potential Source of Helicobacter Pylori in Egypt: Public Health Significance

Elhelw¹,

Elhariri²,

Ragab³

et al. 2020

Preprint

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Background: Helicobacter species is a group of taxonomically related Gram negative, microaerophilic bacteria, which are known to colonize the gastrointestinal and biliary tracts of human and different animal species. The objective of the present study was to determine the occurrence of H. pylori in owned dog and its role in transmission of H pylori to the dog owners. Method: Human gastric biopsies (n=80) and Canine stool (n=60) were collected and examined by nested PCR. The PCR positive samples from human and dog isolates were further subjected to partial 16s sequencing. Result: H pylori was proved in 62.5% and 91.6% of owned dog and dog owners, respectively. The 16s sequencing of the isolates from human and dog were homologous. Conclusion: This study showed higher occurrence of H pylori in both human and dogs in Egypt. Zoonotic transmission between dogs and human is probable and reflects a public health concern.

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An exploratory study on the presence of Helicobacter heilmannii and Helicobacter billis in the feces of companion dogs

Khader

Borujeni

Bakhtiari

2022

Veterinary Medicine & Sci

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Background: Companion animals like dogs play an important role in the lives of many people and are often considered to be members of families, but definitely, any contact with them poses an inherent risk of transmitting zoonotic pathogens. One of these pathogens is the genus Helicobacter which is linked to many disorders in human and animal. Objectives:The aim of this study was to investigate the presence of some zoonotic species of genus Helicobacter in companion dogs.Results: Through culturing in a special medium, nine samples (9%) were detected as infected (two pure and seven mixed culture). Based on multiplex-PCR, 13 samples (13%) were infected by Helicobacter spp. although none of them were infected by H. pylori. Species-specific PCR indicated that 38.5% or 5/13 of the samples were infected with H. heilmannii, while 15.45% or 2/13 of the samples were infected by H. billis. Multivariate logistic regression analysis showed that the age factor had a significant effect on Helicobacter spp. infection (odds ratio [OR] = 2.42, p = 0.01). Conclusion:This study revealed the negligible faecal transmission of H. pylori. Moreover, due to the detection of H. Heilmannii and H. billis in feces and their association with human gastric diseases, dog owners should be educated about the risks and transmission modes of zoonotic bacterial infections of dogs.

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Serologic evidence and risk factors for Helicobacter pylori infection in animals and humans

Cited by 14 publications

References 28 publications

Occurrence of cagA+ vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk

Occurrence of cagA+ vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk

Dog as Potential Source of Helicobacter Pylori in Egypt: Public Health Significance

An exploratory study on the presence of Helicobacter heilmannii and Helicobacter billis in the feces of companion dogs

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