Prevalence of genes encoding virulence factors among Escherichia coli with K1 antigen and non-K1 E. coli strains

Kaczmarek, Agnieszka; Budzyńska, Anna; Gospodarek, Eugenia

doi:10.1099/jmm.0.044263-0

Cited by 31 publications

(27 citation statements)

References 25 publications

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“…This factor occurred in 92 (98%) FeEC isolates as seen in Table 2. Similar results were obtained by previous studies, 18,19 whereas the frequency of the iha gene, as other adhesion, was 26% (24/94). Our laboratory previously showed that the prevalence of iha gene was 29% among UPEC isolates.…”

Section: Discussionsupporting

confidence: 91%

Prevalence of Genes Encoding Outer Membrane Virulence Factors Among Fecal Escherichia coli Isolates

Rashki¹,

Abdi²,

Shookohi³

2017

Int J Basic Sci Med

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Section: Discussionsupporting

confidence: 91%

Prevalence of Genes Encoding Outer Membrane Virulence Factors Among Fecal Escherichia coli Isolates

Rashki¹,

Abdi²,

Shookohi³

2017

Int J Basic Sci Med

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“…Also higher than Beutin et al (1993) Eftekharian et al, (2016) who detected Fim H gene in almost 50% of isolates. Also this result is higher than that reported by Kaczmarek et al, (2012) who reported that approximately 80 % of all E. coli strains of fecal origin are able to produce type 1 fimbriae, which are encoded by the chromosomal Fim operon. On the other hand Iss gene was detected in 100% of isolates of dog and human and 66.7% of isolates of cat.…”

Section: Molecular Detection and Genotyping Of Bacterial Isolates By contrasting

confidence: 55%

Characterization of Helicobacter Pylori and Escherichia Coli From Stool of Human and Pet Animals

Elkassas¹,

Khaliel²,

Shawky³

2018

AJVS

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Key words:Helicobacter pylori, gastritis, Escherichia coli, PCR, Virulence genes, human, dog and catThe recent isolation of Helicobacter pylori from cats and human has potentially important public health implications as well as other bacteria associated with H.pylori in stool samples such as E.coli. A total of 202 stool samples from human, cats and dogs were examined for detection of H.pylori and E.coli. For isolation of H.pylori, 150 stool samples were cultivated on Dent , s medium (modified skirrow , s medium) containing selective Dent , s antibiotic supplement. while the remaining 52 samples were cultivated on MacConkey, Eosine methylen blue media in attempt to isolate other associated bacteria. Colonies grossly similar to H.pylori were tested for catalase, oxidase and urease test. All human samples were tested serologically by using rapid stool antigen test (SAT). PCR assay was performed on bacterial isolates and directly from stool samples by extraction of DNA from stool by boiling method. Positive cultures were subjected to serology testing and PCR. 45samples from humans gave positive results with rapid H.pylori stool antigen test (HPSA). Results revealed that H.pylori was detected in tested samples from human and cat at a rate of 52.8%, 33.3%, respectively and could not be detected in dog. Also detected serologically at percentage of 84.9% in human stool samples. H.pylori could not be detected by PCR, while the other 52 samples were found positive for Escherichia coli, isolation at a percentage of 48%. Nine serogroups of E.coli (O1, O20, O55, O86, O114, O125, and O146) were detected in 9 samples (3 samples from each species). All serotypes were positive for Iss and Fim genes. From all stool samples subjected for isolation of E.coli we obtained 15 isolates which were introduced to PCR. 14 isolates were positive for E.coli genus (phoA gene). 9 isolates were used for detection of virulence genes (Iss, Fim, and Tsh genes). 8 isolates were positive for (Iss and Fim) gene. None was positive for (Tsh) gene. We conclude that H.pylori is very difficult to culture due to the fastidious nature of the organism and its transport and culture requirements. Also H.pylori is the main species of Helicobacter present in human. While dogs and cats harbour many gastric Helicobacter like organisms (GHLOs) and E.coli isolated from human, dogs and cats share the same characters and virulence genes.

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“…3,34,35 The antigenic structure of E.coli is represented by multiple antigens (O), (K) and (H) which in combination account for the genetic diversity of the bacteria. Strains with the K1 antigen have been associated with the development of neonatal sepsis and meningitis as well as with increased risk of mortality when compared with K1 negative strains 36 . Some studies suggest a more aggressive presentation for infants infected with E.coli , with a higher risk of thrombocytopenia and death in the first days of life.…”

Section: Early Onset Sepsismentioning

confidence: 99%

Neonatal Infectious Diseases

Camacho-González

Spearman

Stoll

2013

Pediatric Clinics of North America

341

132

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Synopsis Neonatal sepsis remains a feared cause of morbidity and mortality in the neonatal period. Maternal, neonatal and environmental factors are associated with risk of infection, and a combination of prevention strategies, judicious neonatal evaluation and early initiation of therapy are required to prevent adverse outcomes. The following chapter reviews recent trends in epidemiology, and provides an update on risk factors, diagnostic methods and management of neonatal sepsis.

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Prevalence of genes encoding virulence factors among Escherichia coli with K1 antigen and non-K1 E. coli strains

Cited by 31 publications

References 25 publications

Prevalence of Genes Encoding Outer Membrane Virulence Factors Among Fecal Escherichia coli Isolates

Prevalence of Genes Encoding Outer Membrane Virulence Factors Among Fecal Escherichia coli Isolates

Characterization of Helicobacter Pylori and Escherichia Coli From Stool of Human and Pet Animals

Neonatal Infectious Diseases

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