Prevalence of Extended-Spectrum β-Lactamases and Antibiotic Resistance Patterns in Acinetobacter baumannii Isolated from Clinical Samples in Kermanshah, Iran

Azizi, Mohsen; Mortazavi, Seyed Hamidreza; Etemadimajed, Misagh; Gheini, Simin; Vaziri, Siavash; Alvandi, Amirhooshang; Kashef, Mahsa; Ahmadi, Kamal

doi:10.5812/jjm.61522

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…In the current study, 62.6% of A. baumannii isolates were ESBL producers. Our results were consistent with two other studies in Iran in 2013 in Tabriz and in 2017 in Kermanshah on A. baumannii isolates which showed that 70% and 53.8% of the isolates produced ESBL, respectively ( 32 , 34 ). However, we found a higher prevalence of ESBL-producing A. baumannii compared with other studies in Iran in Hamedan (7%) and Tehran (16%) ( 35 , 36 ).…”

Section: Discussionsupporting

confidence: 93%

“…In one study in Iran, the prevalence of blaTEM and blaCTX genes was 52.1% and 43.4%, respectively, in ESBL-producing A. baumannii isolates (36). Two other studies also reported the presence of blaCTX-M and blaTEM genes in clinical isolated of ESBL-producing A. baumannii isolates (34,37). In 2019, 42% of A. baumannii strains isolated from patients admitted to hospitals in Tehran were positive for the blaTEM gene and negative for the presence of the blaSHV gene (33).…”

Section: Discussionmentioning

confidence: 98%

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Detection of Extended-Spectrum ß-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran

Zarabadi-Pour¹,

Peymani²,

Habibollah-Pourzereshki³

et al. 2021

Ethiop J Health Sci

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BACKGROUND፡ Acinetobacter baumannii is a major contributor to nosocomial infections. Extended-spectrum ßlactamase (ESBL)-producing A. baumannii is spreading worldwide. We aimed to determine the frequency of ESBLencoding genes in clinical isolates of A. baumannii and to access their clonal relationship by repetitive extragenic palindromic-PCR (rep-PCR).METHODS: In this descriptive cross-sectional study, 203 isolates of A. baumannii were collected from Qazvin hospitals. The Identification of isolates was performed by standard laboratory methods. To verify ESBL production, all isolates were screened by disk agar diffusion and confirmed by the combined disk method. Subsequently, ESBL-encoding genes were detected by PCR and sequencing. Possible clonal association of ESBL-producing isolates was evaluated using rep-PCR.RESULTS: Two hundred (98.5%) isolates showed reduced susceptibility to one of the antibiotics used in the ESBL screening test, of which 127 isolates (62.6%) produced ESBL. PCR results showed blaOXA-1 (20.5%) was the most prevalent gene followed by blaTEM-1 (20%), blaGES-1 (15.7%), blaCTX-M-15 (7.9%), and blaPER-1(1.6%). Rep-PCR results revealed that ESBL-producing isolates belonged to clones A (85%), B (13.4%), and C (1.6%).CONCLUSION: Our study showed the significant presence of blaOXA-1, blaTEM-1, blaGES-1, blaCTX-M-15, and blaPER-1 genes in ESBLproducing A. baumannii isolates in the studied hospitals. This is the first report on the emergence of blaOXA-1 gene in these isolates in Iran. The use of comprehensive antimicrobial treatment guidelines based on laboratory data and appropriate infection control interventions are essential.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Detection of Extended-Spectrum ß-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran

Zarabadi-Pour¹,

Peymani²,

Habibollah-Pourzereshki³

et al. 2021

Ethiop J Health Sci

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“…The presence of class-D OXA carbapenemase genes has been linked to the vigorous biofilm growth caused by A. baumannii (4). Class I integrons are found across the MDR A. baumannii strain population in high numbers (5).…”

Section: Introductionmentioning

confidence: 99%

Effects of physicochemical changes (temperature, pH, and culture media) on strong biofilm formation of Acinetobacter baumannii isolated from patients with respiratory infection in Iraq

Mutashar¹,

Al-Mudallal²,

Ridha³

2023

Biomedicine

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Introduction and Aim: The ability of Acinetobacter baumannii to form biofilms on biotic and abiotic surfaces is regulated by several pathogens’ virulence factors, and this is thought to be at the root of the bacteria's resistance to antibiotics. We hope to learn how temperature, pH, and iron concentrations influence the development of biofilms in A. baumannii isolated from COVID-19 and non-COVID-19 individuals, and which genes are relevant for biofilm formation and antibiotic resistance. Materials and Methods: Eight strong adherent isolates of A. baumannii from respiratory tract infection Iraqi patients (4 from COVID-19 and the other from non-COVID-19 just respiratory patients) had been used in this study which conducted from 10/1/2021 to 10/2/2022. The antibiotic sensitivity of all isolates was determined using the VITEK-2 system. The biofilm associated genes OXA-51, bap, Chaperone Usher (CsuE) and Integron-1, was detected using PCR. Isolates of A. baumainni were put through a battery of tests to determine whether they possessed the capacity to produce robust biofilms under a wide range of both physical (temperature, pH) and chemical circumstances. Results: A. baumannii showed that all isolates were multidrug resistant and positive for the biofilm genes studied. Effect on temperature on biofilm formation showed at 44ºC biofilm formation was significantly lower than that at 37ºC (mean differences of 0.178000 (t= 8.355, df:3, P=0.004) and 0.204000 (t=26.521, df:3, P=0.000) respectively). The adhesion factor value in the COVID-19 positive and negative groups decreased significantly because of the pH change. Iron concentration of 60 µM significantly lowered biofilm formation among COVID-19 group and non-COVID-19 group. Conclusion: A. baumanni are multidrug resistance isolates with a capacity to form biofilms. The ability to form biofilms by A. baumannii is strongly influenced by physical and chemical factors.

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“…Different species of this genus can cause various nosocomial infections, commonly among patients in intensive care units (ICUs) and highdependency units (HDUs) [3]. Acinetobacter baumannii is more prevalent than other strains of genus Acinetobacter, and it is the most common cause of bacterium-induced infections [4]. Ventilatorassociated pneumonia (VAP), septicemia, secondary meningitis, endocarditis, infections of the skin, soft tissues, urinary tract, and wound infection can be caused by bacteria A. baumannii bacteria [5].…”

Section: Introductionmentioning

confidence: 99%

“…To treatment of A. baumannii infections, various antibiotics, including β-lactams, uoroquinolones, and aminoglycosides, are used. Today, many studies have shown that antibiotic resistance in the A. baumannii isolates is extremely increasing [3,4,6,7]. Inappropriate and indiscriminate use of available antibiotics, high ability of natural genetic transformation in A. baumannii bacteria and its potential for widespread dissemination are most important factors contributed to the resistance of this pathogen to the various antimicrobial agents [8,9].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Patterns and Distribution of Ampc β-Lactamases Genes Among Acinetobacter Baumannii Clinical Isolates from Hospitals of Tehran, Iran

Alizadeh

Baserisalehi

Bahador

2021

Preprint

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Background Acinetobacter is a Gram-negative coccobacilli bacterium that can produce severe and different infections. Among the species of these bacteria, Acinetobacter baumannii is the most common cause of nosocomial infections. Due to the high prevalence of antibiotic resistance in this bacterium and the significant increase in antibiotic resistance, this study was conducted to investigate the antibiotic susceptibility of A. baumannii isolates from Iran. A total of 60 A. baumannii bacteria were isolated from the different clinical samples in hospitals of Tehran, Iran. The isolates susceptibility to 13 commonly used antibiotics was examined according to the Clinical & Laboratory Standards Institute (CLSI) guidelines. Using PCR, three important AmpC β-lactamases relayed genes (DHA, CIT and MOX) were detected. Results The highest and lowest resistance rate was related to ampicillin (98.3%) colistin (35%), respectively. Of 60 isolates, 59 isolates (98.34%) were resistant to more than 8 antibiotics. The frequencies of DHA, CIT and MOX genes were 1 (2%), 7 (12%), 27 (46%), respectively. Based on definition, 59 (88.33%), 44 (73.33%) and 13 (21.66%) isolates were MDR, XDR and PDR, respectively. Twenty-four isolates (40%) were negative for all three genes. There was a significant relationship between the presence of MOX gene and antibiotic resistance. Conclusions The high resistance rates of the A. baumannii isolates reported in the present study is alarming and need the management of treatment such as performing of antibiogram test before antibiotic therapy for select the appropriate antibiotic and also completes the course of treatment period.

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Prevalence of Extended-Spectrum β-Lactamases and Antibiotic Resistance Patterns in Acinetobacter baumannii Isolated from Clinical Samples in Kermanshah, Iran

Abstract: Background: Acinetobacter is an opportunistic bacterial pathogen with intrinsic and acquired resistance to many antibiotics.

Cited by 7 publications

References 21 publications

Detection of Extended-Spectrum ß-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran

Detection of Extended-Spectrum ß-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran

Effects of physicochemical changes (temperature, pH, and culture media) on strong biofilm formation of Acinetobacter baumannii isolated from patients with respiratory infection in Iraq

Antibiotic Resistance Patterns and Distribution of Ampc β-Lactamases Genes Among Acinetobacter Baumannii Clinical Isolates from Hospitals of Tehran, Iran

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