Prevalence of extended-spectrum β-lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital

Abstract

The present study was undertaken to investigate the high incidence of multiresistant Gram-negative bacilli causing neonatal septicaemia. Samples of neonatal blood from 728 suspected cases were obtained in brain heart infusion broth with sodium polyanethol sulfonate. All Gram-negative rods isolated were subsequently subjected to routine antimicrobial susceptibility testing and tests for extended-spectrum â-lactamase (ESBL) production, as per NCCLS recommendations. ESBL was detected in 86·6 % of Klebsiella spp., 73·4 % of Enterobacter spp. and 63·6 % of Escherichia coli strains. It was also observed that 74·4-80·9 % of these ESBL producers were resistant to cefotaxime and 47·6-59·5 % were resistant to ceftazidime in routine susceptibility testing. Some ESBL producers (36·3-61·5 %) were found to be susceptible to either or both cephalosporins used in this study. It is concluded that indiscriminate use of third-generation cephalosporins may be responsible for the selection of ESBL-producing multiresistant strains in the neonatal intensive-care unit (NICU).

Prevalence, antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and Klebsiella oxytoca among hospitalized patients

“…Such high rate of ESBL among K. pneumoniae might be due to inappropriate use of antibiotics and self-medication against K. pneumoniae infection. However, our results are significantly better for ESBL producing K. pneumoniae than found by Afifi (2013) in Egypt (56.25%), Jain et al (2003) in India (86.6%) and Ullah et al (2009) in Pakistan (58.5%) (1,16,37).…”

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Gram Negative Bacilli from various clinical isolates

“…Patient to patient transfer of ESBL producing strains of Klebsiella spp. could also be another reason as has been shown by Jain et al [21] in their study. In our study the prevalence of E. coli was observed to be 62 % which was in accordance with studies conducted by Shivprakasha et al (62.34%) [18] , and Gupta et al (63.79%) [19] .…”

Frequency and Antimicrobial Sensitivity Pattern of Extended Spectrum β-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from urine at a Tertiary Care Hospital

“…The incidence of ESBL in major hospitals of India has been reported to be as high as 58% 19 . The range of ESBL isolation rate has been varied from 6 to 39% in different studies 20,21,22,23 25,26 was reported. The high incidence of ESBLs among E. coli may be peculiar to the Indian subcontinent.…”