Frequency and Antimicrobial Sensitivity Pattern of Extended Spectrum &amp;beta;-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from urine at a Tertiary Care Hospital

Islam, Md. Badrul; Jhora, ST; Yusuf, Abdullah; Sattar, Afma; Shahidullah, S; Rahman, Nmw; Sharif, AR; Ahsan, AI; Wadud, Abma; Chowdhury,

doi:10.3329/jssmc.v4i1.11999

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…The isolates of P. vulgaris showed resistance in 100% of the cases, but this result must be taken conditionally due to the small number of isolates. This is consistent with the results from other Eastern European countries [63][64][65] , and in contrast with those from Asian countries 59,66 .…”

Section: Resultssupporting

confidence: 92%

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Prevalence of Extended Spectrum Beta-Lactamases among Enterobacteriaceae Isolated from Intrahospital Patients in Serbia

Ciric¹,

Stanisic²,

Milosevic³

et al. 2018

Current Science

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The aim of this study was to determine the prevalence of extended spectrum beta-lactamases (ESBLs) production in hospital strains of Enterobacteriaceae isolated from various clinical specimens (urine, blood and wound swabs) from hospitalized patients at the Military Medical Academy, Belgrade, Serbia. During six months of study, a total of 1034 isolates of Enterobacteriaceae were tested for antimicrobial susceptibility and screened for ESBL production according to standard methods. The overall prevalence of ESBL production in the hospital isolates of Enterobacteriaceae was 57.4%. Among the isolates, minimum frequency of resistance was found for amikacin (25.2%), and maximum for ampicillin (84.5%). The strain resistant to imipenem could not be isolated. Resistance to the tested antibiotics was higher in ESBL producers than non-producers (P < 0.05). Among Escherichia coli isolates, the prevalence of ESBL production was less than 50% (33.9%). ESBLs were most often produced by isolates of Serratia spp. (85.2%) and Klebsiella spp. (81.8%). Blood specimens were the most common sources of ESBL-producing isolates (84.0%). These findings might help clinicians in deciding the appropriate empirical treatment for intrahospital patients and emphasize the increasing problem of antimicrobial resistance in Serbia.

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Section: Resultssupporting

confidence: 92%

“…Results of the present study are consistent with those from the literature highlighting that prevalence of ESBL strains of K. pneumoniae is significantly higher than that of E. coli, in the European countries and USA [55][56][57][58] . The situation is reversed in the countries of Asia, Africa and South America 43,46,59,60 .…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Extended Spectrum Beta-Lactamases among Enterobacteriaceae Isolated from Intrahospital Patients in Serbia

Ciric¹,

Stanisic²,

Milosevic³

et al. 2018

Current Science

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“…Our study showed a susceptibility of 100% for imipenem, this frequency was similar with other studies in which susceptibility varied from 95 up to 100% for imipenem. 18,20,25,30,31,36 We have few options for treating severe infections because of the significant rise in b-lactamase production, which includes the high level of ESBL producers. In this study, the ESBL strains showed high resistance to ampicillin (100%), aztreonam (78.5%), cefepime (100%), ceftriaxone (86.6%), tetracycline (66.66%), norfloxacin (50%) and nitrofurantoin (8.33%), which is consistent with a claim made by Cristea et al in Romania.…”

Section: Discussionmentioning

confidence: 99%

Genomic Determinants and Antimicrobial Resistance Pattern of Clinical Isolates of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli

Shah,

Syed,

Bhat

et al. 2023

J. Pure Appl. Microbiol.

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The growing prevalence of antibiotic-resistant bacteria is a worldwide public health apprehension, and Escherichia coli (E. coli) is one of the most commonly implicated bacterial species. Among E. coli isolates, extended-spectrum β-lactamase (ESBL)-producing strains have been identified as a key contributor to antibiotic resistance. Penicillin’s, cephalosporins, and monobactams are only a few of the β-lactam antibiotics that can be rendered inactive by ESBLs. This investigation’s goals were to determine the prevalence of ESBL-producing E. coli isolates found in clinical samples and to analyze the distribution of the blaTEM, blaSHV, and blaCTX-M genes among them. Additionally, we aimed to determine the antibiotic susceptibility patterns of these isolates to other antibiotics. Clinical isolates from urine, ear swab, and wound/pus swabs were collected from patients with suspected E. coli bacterial infections from different regions of north India viz., SKIMS-JVC medical college and NABL accredited Dr. Qadri’s Lab both from Srinagar Kashmir valley region and SMSR, SU from Greater Noida UP. Standard laboratory techniques were used to identify E. coli isolates, and the combined disc method and other phenotypic confirmation techniques were used to confirm ESBL formation. PCR amplification and sequencing were used to find the blaTEM, blaSHV, and blaCTX-M genes. The Kirby-Bauer disc diffusion method was used to test the antimicrobial susceptibility of various bacteria to different antibiotics. A total of 210 E. coli isolates were collected from different clinical samples and only 158 isolates showed positive results for ESBL by DDST and phenotypic confirmatory tests. Of these, 124 (78.48%) were ESBL-producing isolates. We found that blaTEM was the most prevalent gene (45.16%), followed by blaCTX-M (34.16%) and blaSHV (12.09%). Antimicrobial resistance profiles were assessed for each of the 120 isolates. Ampicillin and Cefepime were the most resistant drugs to ESBL-producing isolates, followed by Gentamicin, Ceftriaxone, and Cefixime.

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Higher prevalence of multidrug-resistant extended-spectrum β-lactamases producing Escherichia coli in unorganized pig farms compared to organized pig farms in Mizoram, India

et al. 2020

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Aim: The present study was conducted to record the prevalence of multidrug-resistant (MDR), extended-spectrum β-lactamases (ESBLs) producing Escherichia coli from pig population of organized and unorganized farms of Mizoram and to record the presence of ESBLs, non-ESBLs, and integrons. Materials and Methods: Fecal samples were collected from pigs under organized (n=40) and unorganized (n=58) farms of Mizoram. Samples were processed for isolation and identification of E. coli by conventional techniques, BD Phoenix™ automated bacterial system, and polymerase chain reaction (PCR) based confirmatory test. All the isolates were subjected to antimicrobial sensitivity test by disk diffusion assay and ESBLs production by double-disk synergy test (DDST). The ESBLs producing isolates were subjected to PCR for determination of ESBLs genes and all the isolates were screened for non-ESBLs genes and integrons by PCR. Results: A total of 258 E. coli was isolated and identified from organized (n=120) and unorganized farms (n=138). Majority of the E. coli isolates exhibited high level of resistance against amoxicillin (Ax) (81.78%), cefalexin (85.42%), co-trimoxazole (50.78%), sulfafurazole (69.38%), tetracycline (65.89%), and trimethoprim (TR) (51.94%). Statistically highly significant (p<0.01) variations in resistance among the isolates from organized and unorganized farms were recorded in case of Ax, ampicillin, cephalexin, ciprofloxacin, co-trimoxazole, gentamicin, piperacillin, and TR. By DDST, 65.89% isolates were recorded as ESBLs producer, of which 82/120 (68.33%) and 88/138 (63.77%) were from organized and unorganized farms, respectively. A total of 29/258 (11.24%) isolates were positive for at least one ESBLs gene. blaTEM was most frequently (9.69%) gene, followed by blaCTX-M (5.04%) and blaCMY (0.78%). Altogether, 6 (5.00%), 4 (3.33%), and 2 (1.67%) isolates from the organized farms were positive for blaCTX-M, blaTEM, and blaCMY genes, respectively. Similarly, 21 (15.22%) and 7 (5.07%) isolates from the unorganized farms were positive for blaTEM and blaCTX-M genes, respectively. None of them were positive for blaSHV genes. Altogether 57 (22.09%), 9 (3.49%), 66 (25.58%), 78 (30.23%), 21 (8.14%), and 18 (6.98%) isolates were positive for tetA, tetB, sul1, sul2, aadA, and dfrla genes, respectively. The prevalence of non-ESBLs genes was higher in the E. coli isolates from the unorganized farms than organized farms. Conclusion: MDR and ESBLs producing E. coli are circulating among the pigs and their environment in Mizoram. Pigs under unorganized farms exhibited higher level of resistance against majority of the antimicrobials, including third-generation cephalosporins, which might be an indication of overuse or misuse of antibiotics under the unorganized piggery sectors in Mizoram.

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Frequency and Antimicrobial Sensitivity Pattern of Extended Spectrum β-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from urine at a Tertiary Care Hospital

Cited by 3 publications

References 15 publications

Prevalence of Extended Spectrum Beta-Lactamases among Enterobacteriaceae Isolated from Intrahospital Patients in Serbia

Prevalence of Extended Spectrum Beta-Lactamases among Enterobacteriaceae Isolated from Intrahospital Patients in Serbia

Genomic Determinants and Antimicrobial Resistance Pattern of Clinical Isolates of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli

Higher prevalence of multidrug-resistant extended-spectrum β-lactamases producing Escherichia coli in unorganized pig farms compared to organized pig farms in Mizoram, India

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