Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

Schmid, A.; Hörmansdorfer, Stefan; Messelhäußer, Ute; Käsbohrer, Annemarie; Sauter‐Louis, Carola; Mansfeld, Rolf

doi:10.1128/aem.00204-13

Cited by 127 publications

(158 citation statements)

References 38 publications

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“…The 100% resistance to ESBL in this study is similar to the findings of Duru et al (2013) among ESBLs-producing E. coli isolates from poultry in Imo State, Nigeria. The 100% cefotaxime resistance observed in this study is higher than 84.6% cefotaxime resistance reported by Schmid et al (2013) among ESBLsproducing E. coli isolates from beef cattle in Germany. High rates (100%) of resistance to ampicillin and aztreonam among all the genera of enterobacterial isolates were observed in the hereby experiment, further suggesting the production of ESBLs by the isolates.…”

Section: Discussioncontrasting

confidence: 70%

“…Conversely, the ESBLs are inhibited by clavulanic acid and other β-lactamase inhibitors (GarciaGraells et al, 2010;Ben Sallem et al, 2012). Amoxicillin/clavulanic acid resistance rate (100%) in this study is higher when compared with 53.8, 6.7 and 90% amoxicillin/clavulanic acid resistance reported by Schmid et al (2013), Guandong and Avci (2013) and Duru et al (2013) among ESBL-producing E. coli isolates from cattle in Germany, food of animal origin in Turkey and poultry in Nigeria, respectively. It is also higher than 3.1% amoxicillin/clavulanic acid resistance among ESBLsproducing Klebsiella isolates reported by Guandong and Avci (2013).…”

Section: Discussionmentioning

confidence: 46%

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Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

et al. 2015

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This study was conducted to isolate generic extended-spectrum β-lactam (ESBL)-resistant enterobacteria from pigs reared in Enugu State Southeast, Nigeria and determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 190, randomly selected, apparently healthy pigs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 2 µg/ml of cefotaxime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 46 ESBL-resistant enterobacterial isolates, 4 (8.7%) were Escherichia coli, 11 (23.9%) were Salmonella species, while 31 (67.4%) were Klebsiella species. Resistance of the Salmonella isolates was 45.5% to ciprofloxacin, 36.4% to ofloxacin and levofloxacin, 9.1% to norfloxacin, amikacin and gentamicin, 27.3% to streptomycin, 72.7% to chloramphenicol and 90.9% to tetracycline. Resistance of the Klebsiella isolates was 93.5% to ampicillin, 12.9% to ciprofloxacin, 19.4% to ofloxacin and levofloxacin, 9.7% to norfloxacin and streptomycin, 64.5% to chloramphenicol and 38.7% to tetracycline. Resistance of the E. coli isolates was 100% to gentamicin, 75% to ampicillin and streptomycin, 50% to ciprofloxacin, norfloxacin, chloramphenicol and tetracycline, and 25% to ofloxacin, levofloxacin and amikacin. All the isolates were resistant to ceftriaxone, cefotaxime, ceftazidime, cefepime, cefpodoxime, amoxicillin/clavulanic acid and aztreonam. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 54.8% for Klebsiella, 90.9% for Salmonella and 100% for E. coli, respectively. This study has shown that pigs reared in Enugu State Southeast, Nigeria, are colonized by ESBL-resistant Enterobactericeae and are potential reservoirs and disseminators of these organisms.

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Section: Discussioncontrasting

confidence: 70%

Section: Discussionmentioning

confidence: 46%

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

et al. 2015

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“…For example, in Germany, Schimid et al in a study carried out in the year of 2013 they highlighted a prevalence of 56,2% of E.coli ESBL positive isolated strains in the faeces derived from calves, followed by the prevalence obtained for the cows of 41,1% and of 21,4% for the cows of beef (Schimid et al, 2013). In France, in the year of 2014, Haenni et al, signalled a prevalence of 29, 4% of the calves which produced E.coli ESBL positive strains in the faecal flora (Haenni et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

The Prevalence of Esbl-Producing Strains of E.coli, Isolated from Calves with Colibacilosis - Preliminary Remarks

Cozma¹,

Crivei²,

Ciocan³

et al. 2017

BUASVMCN-VM

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The animals producing food have become an increasing reservoir of extended spectrum beta-lactamase producing Enterobacteriaceae. The calves and cows are exposed to a greater quantity of antibiotics, but the data concerning the prevalence of ESBL-producing Enterobacteriaceae are not enough, in comparison with other species of animals used for human consumption, such as birds (Hordijk et al., 2013).The aim of this study was to determine the prevalence of ESBL-producing E. coli involved in some episodes of colibacilosis in calves. Faeces samples were collected from 33 calves with the age ranging between 1-2 weeks and that presented clinical signs of colibacilosis. The samples were collected in a sterile medium for the taxonomic isolation and identification of the etiological agent involved, the ESBL screening being conducted subsequently using the ESBL Agar Oxoid Brilliance chromogenic medium. The phenotypic confirmation of the ESBL-producing strains was conducted in accordance with the CLSI (2014) standard through the combined disc method. Following the tests conducted, out of the 33 strains of isolated E. coli, 9 (27, 27%) were phenotypically confirmed as being ESBL strains.The studies that were previously conducted on the dairy farms have pointed out that the young calves rapidly acquire bacterial strains resistant to antibiotics that are often ESBL strains (Hordijk et al., 2013). The prevalence obtained by us, as well as an insufficient quantity of information concerning the antimicrobial resistance on this segment of species of animals used for the human consumption, support conducting a more thorough study, as well as the identification of ESBL resistance genes, but also of the plasmids that encode the transmission of these genes.

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“…Animals being reared either as pets or for consumption have been identified as potential reservoirs of resistant fecal flora (Schmid et al, 2013). Fecal flora is comprised largely of the Enterobacteriaceae family and one major mechanism of resistance among these Gram negative rods is the production of extended spectrum beta-lactamase (ESBL) (dos Santos et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Extended spectrum betalactamases (ESBLs) are enzymes that inactivate a large number of β-lactam antibiotics including extendedspectrum very-broad-spectrum cephalosporins and monobactams (Paterson and Bonomo, 2005). The genetic determinants of ESBL enzyme are largely plasmid borne, being of TEM, SHV, OXA and CTX-M genes predominantly with the propensity for transfer between species (Paterson and Bonomo, 2005;Schmid et al, 2013). They are inhibited by clavulanic acid, sulbactam and tazobactam, and have been reported to coexist with resistance to other antimicrobial classes like aminoglycosides and quinolones (Paterson and Bonomo, 2005).…”

Section: Introductionmentioning

confidence: 99%

Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

Ogefere¹,

Agbe²,

Ibadin³

2017

Not Sci Biol

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This study was carried out to determine the prevalence of extended spectrum beta-lactamase (ESBL) among Gram negative bacteria isolated from cattle feces in Benin City, Nigeria. A total of 250 Gram negative bacteria isolates were recovered from cattle feces and were processed microbiologically using standard techniques. Emergent colonies were identified and antibacterial susceptibility tests were determined using Kirby-Bauer disk diffusion method. All bacterial isolates were screened for the presence of ESBL using the double-disc synergy method. A total of 37 (14.8%) isolates were positive for ESBL, with 33 (13.2%) indicated by ceftazidime, while only 4 (1.6%) were indicated by both ceftazidime and cefotaxime (P < 0.0001). Of the Gram negative bacterial isolates recovered, Salmonella species was the most prevalent ESBL-producer with 55.0% prevalence (P = 0.0092), while no isolate of Pseudomonas aeruginosa produced ESBL. ESBL-positive isolates showed poor susceptibility to the tested antibacterial agents in comparison with non-ESBL-producers and imipenem was the most active antibiotic. The prevalence of ESBL among Gram negative bacilli recovered from cattle feces was 14.8%. The study advises prudent use of antibiotics in the treatment of cattle and harps on improved hygiene in managing cattle, as they are potential reservoirs of ESBL-producing organisms.

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Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

Cited by 127 publications

References 38 publications

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

The Prevalence of Esbl-Producing Strains of E.coli, Isolated from Calves with Colibacilosis - Preliminary Remarks

Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

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