Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

Ogefere, Helen Oroboghae; Agbe, Salome O.; Ibadin, Ephraim Ehidiamen

doi:10.15835/nsb9210005

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…South Africa (CTX-M), Madagascar (CTX-M-15), Nigeria (CTX-M and ND-ESBL), Tanzania (CTX-M-15) and Tunisia (CTX-M-15) also presented reports of E-ESBL in cattle. The reports were E. coli, Klebsiella sp, Salmonella sp, P. mirabilis, P. vulgaris, Providencia sp e Shigella spp (Gay et al, 2018;Iweriebor et al, 2015;Ogefere et al, 2017;Olowe et al, 2015;Saidani et al, 2018;Seni et al, 2016). In all countries the origin was faecal (except Tunisia, with mastitis also).…”

Section: Africamentioning

confidence: 99%

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

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Section: Africamentioning

confidence: 99%

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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“…A retrospective study conducted in Switzerland, reported 60% ESBL-producing E. coli occurrence among 60 clinical E. coli isolates from different samples of horses (26), while a Dutch study observed 99.5% ESBL-producing E. coli occurrence among 198 faecal/wound E. coli isolates from horses (2); these findings are lesser than the result (100%) of the current study. Previous studies in Nigeria (which did not use ESC-amended media for primary isolation) reported 16.3-44.7% and 8.9-22.5% ESBL-producing E. coli occurrence among E. coli isolates from cattle (34,35) and chickens (29,31,33), respectively. These results are also lesser than the findings (100%) of this study.…”

Section: Discussionmentioning

confidence: 98%

“…There are a number of reports on isolation of ESC-resistant/ESBL-producing enterobacteria from clinical samples of horses (2,17,18,(22)(23)(24)(25)(26), but surveillance studies assessing healthy horses as potential reservoirs and disseminators of ESCresistant/ESBL-producing enterobacteria are rather scanty and included reports in the United Kingdom (27,28) and Czech Republic (17). In Nigeria, investigators have screened animals such as chickens (29)(30)(31)(32), ruminants (33)(34)(35), pigs (13,34) and dogs (36) as potential reservoirs of ESC-resistant/ESBL-producing enterobacteria. No study has been conducted to screen horses in Nigeria as potential reservoirs of ESC-resistant/ ESBL-producing enterobacteria.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Antibiogram of Generic Extended-Spectrum Cephalosporin-Resistant and Extended-Spectrum β-Lactamase-Producing Enterobacteria In Horses

Anyanwu

Ugwu

Onah

2018

Macedonian Veterinary Review

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This study was conducted to isolate generic extended-spectrum cephalosporin (ESC)-resistant and extended-spectrum β-lactamase (ESBL)-producing enterobacteria from horses in Nigeria, and to determine the antibacterial resistance profile. Rectal swabs were collected from 155, systematic randomly selected, apparently-healthy horses. Isolation of ESC-resistant enterobacteria was done using Mac Conkey agar with ceftazidime. ESBL production was assessed by combination disc method. Resistance of the isolates was determined using disc diffusion method. Out of 155 samples, 5.2% gave positive growth. From these, 11 ESC-resistant enterobacteria comprising of 36.4% E. coli, 36.4% Salmonella spp. and 27.2% Proteus spp., were obtained. From 11 isolates, 45.5% consisting of all the 4 E. coli and 1 Proteus isolates, were ESBL-producers, these were recovered from 4 (2.6%) out of the 155 horses sampled. Resistance of the E. coli isolates was 25% to aztreonam (AZT), 75% to amoxicillin-clavulanic acid (AMC), gentamicin (GEN), perfloxacin (PEF), and sulphamethoxazoletrimethoprim (SXT-TRI), 50% to ofloxacin (OFL) and 100% to ampicillin (AMP), ceftazidime (CTZ), cefotaxime (CTX), chloramphenicol (CHL), streptomycin (STR), tetracycline (TET), sparfloxacin (SPA), ciprofloxacin (CIP), norfloxacin (NOR) and enrofloxacin (ENR). Resistance of the Salmonella isolates was 50% to PEF and 100% to CTZ, CTX, AMP, AZT, AMC, CHL, GEN, STR, TET, SPA, CIP, OFL, NOR and ENR. Resistance of the Proteus isolates was 25% to AMC, CHL, STR, TET, SPA and NOR, and 100% to CTZ, CTX, AZT and AMP. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 75% for Proteus and 100% for E. coli and Salmonella, respectively. This study showed that horses in Nigeria are potential reservoirs and disseminators of ESC-resistant and ESBL-producing Enterobacteriaceae.

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“…[20] and Ogefere et al [21] in Nigeria, Bhoomika et al [22] in India; and Petternel et al [23] in Austria each of whom respectively reported the presence of ESBL producing E. coli (22.5 %, 66.3 %, and 100.0 % and 16.3 %, 12.0 %, 10.99 % and 20 % respectively) in their samples. However, the 44.0% ESBL producing E. coli observed in this study is much lower than 66.3%and 100.0% but much higher than 12.0%, 16.3%, 22.5%, 10.99% and 20% respectively reported by same authors.…”

Section: Discussionmentioning

confidence: 99%

Detection of Extended Spectrum Beta-Lactamase Production in Escherichia coli Isolated from Cattle Faeces in Owo Metropolis

Adeluwoye-Ajayi¹,

Thomas²,

Awoniyi³

2021

IJPR

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Escherichia coli which synthesize extended spectrum beta-lactamases (ESBL) have been implicated in severe human diseases. There is substantial evidence that cattle faeces have a role in developing and spread of multidrug-resistant pathogens, raising public health concerns. The study is aimed at detecting ESBL-producing E. coli associated with cattle faeces within Owo metropolis. Freshly passed faecal samples were taken aseptically from 9 apparently healthy cattle that were about to be slaughtered and placed in correctly labeled sterile capped universal bottles with sterile spatula. Using standardized method, Escherichia coli was isolated on Eosin Methylene Blue Agar. The antibiotic susceptibility of the isolates was evaluated using the Kirby Bauer disc diffusion method, and the expression of ESBL was detected by the double disc synergy test. According to the findings, 25 (55.6%) of the 45 E. coli isolates showed probable production of ESBL during screening and 11 (44.0%) were confirmed to be ESBL producers. The isolates' antibiotic resistance pattern revealed that they were most resistant to gentamicin (81.8%) and least resistant to imipenem (9.1%). As a result, imipenem was the most effective antibiotic against ESBL-producing E. coli isolates. Antibiotypes of ESBL-producing E. coli isolates also showed that 9 (81.8%) of ESBL-positive E. coli isolates were multidrug-resistant. This studies confirmed that E. coli isolated from bovine faeces had high prevalence of antibiotic resistance and revealed that ESBL-producing bacteria are present in cattle and subsequent consumers. This necessitates the implementation of mitigating strategies to limit the transfer of antibiotic-resistant bacteria from animals to humans.

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Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

Cited by 3 publications

References 15 publications

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Occurrence and Antibiogram of Generic Extended-Spectrum Cephalosporin-Resistant and Extended-Spectrum β-Lactamase-Producing Enterobacteria In Horses

Detection of Extended Spectrum Beta-Lactamase Production in Escherichia coli Isolated from Cattle Faeces in Owo Metropolis

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