Prevalence of extended-spectrum β-lactamase in Pseudomonas aeruginosa in tertiary care centre

Abstract

an opportunistic nosocomial pathogen, its increasing resistance to broad-spectrum beta-lactams, mediated by extended-spectrum betalactamase enzymes (ESBL), is problem worldwide. The present study was undertaken to determine the prevalence of ESBL-production among the clinical isolates of Pseudomonas aeruginosa. Various clinical specimens received in our laboratory were processed and Pseudomonas aeruginosa was identified as per standard microbiological procedure. All isolates were subjected for ESBL screening test. Potential ESBL producer was then subjected for ESBL Phenotypic confirmatory test –Disc Diffusion method. Antimicrobial susceptibility test was performed by Kirby – Bauer disc diffusion method on all confirmed isolates as per Clinical Laboratory Standard Institute (CLSI 2016) guidelines. A total of 322 non duplicate isolates of Pseudomonas aeruginosa identified during the study period. Of these 26.09% (n = 84) of Pseudomonas aeruginosa isolates were found to be ESBL producers. All ESBL positive isolates showed high resistance to ciprofloxacin 79 (94.05%), Gentamicin 61 (72.62%) and tobramycin 60 (71.43%). Resistance was low to drugs like cefoparazone + salbactum 17 (20.24%) and piperacillin + Tazobactum 14 (16.67%), and Imipenem 15 (17.86%). All the isolates showed 100% sensitive to Polymyxin B.Present study highlights the prevalence and drug resistance of ESBL positive Pseudomonas aeruginosa. Regular antimicrobial susceptibility monitoring is essential for judicial use of antibiotics in order to prevent the spread of drug resistance.