Prevalence of Escherichia Coli 0157: H7 and Enterobacteriaceae on Hands of workers in Halal Cattle Abattoirs in Peninsular Malaysia

Bm, Shamsul; Adamu, M.T.; Desa, Mohd Nasir Mohd; Khairani-Bejo, Siti

doi:10.21315/mjms2016.23.5.9

Cited by 7 publications

(7 citation statements)

References 15 publications

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“…All the biochemically confirmed E. coli isolates were additionally screened on cefixime tellurite sorbitol MacConkey agar (CT-SMAC) (Merck, Germany). E. coli O157: H7 serotypes appeared colorless (non-sorbitol fermenters), while non-O157: H7 seemed to be pink (sorbitol-fermenters) as described by previous researchers ( 20 – 23 ). Biochemically confirmed E. coli isolates were pre-enriched by BHI broth (Oxoid, UK) at a ratio of 1:10 and with 20% glycerol stored at −80 °C for further procedures.…”

Section: Methodssupporting

confidence: 65%

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

Khan¹,

Rashid²,

Ramli³

et al. 2022

MJMS

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Background: Diarrhoeagenic verotoxin producing non-O157 Escherichia coli (VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children. Methods: Standard microbiological techniques identified a total of 137 non-repeated, clinically significant E. coli isolates. Serological assays discerned non-O157 E. coli serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR). Results: Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157 E. coli. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing E. coli¸ genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates. Conclusion: This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children.

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Section: Methodssupporting

confidence: 65%

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

Khan¹,

Rashid²,

Ramli³

et al. 2022

MJMS

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“…[9][10][11][12] All of the ready to eat food samples of our investigation were meat-based ready to eat foods. Some of them like sausage, salami and soup were Article produced in high temperature and therefore were free from any pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Shiga-toxigenic Escherichia coli in ready-to-eat food staffs: Prevalence and distribution of putative virulence factors

Shahreza

Rahimi

Momtaz

2017

Microbiol Res (Pavia)

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Lack of proper hygiene and using from low quality raw materials cause high presence of food-borne pathogens in ready to eat foods. Shiga toxin producing Escherichia coli is one of the most common cause of food-borne diseases in the world. The present research was done to study the prevalence and distribution of virulence factors in the STEC strains isolated from various types of ready to eat food samples. Seven-hundred and twenty food samples were collected and cultured. Isolated E. coli bacteria were approved another time using the 16S rRNA-based PCR amplification. Approved strains were subjected to multiplex PCR for identification of putative virulence factors. Twenty-six out of 720 food samples (5.20%) were positive for E. coli. Salad (15%), candy (12.50%) and barbecue (10%) were the most commonly contaminated. Prevalence of STEC strains was 2.63%. Prevalence of EHEC and AEEC subtypes were 36.84% and 52.63%, respectively. EHEC strains harbored all three stx1, eae and ehly genes. High presence of EHEC strains besides the considerable distribution of multiple virulence factors showed an important public health issue regarding the consumption of ready to eat foods.

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“…Furthermore, the lower number of E. coli isolated from the swab sample of workers hand after work as compared to samples before work is another surprising result in this study. Actualy, it is hypothesized that the presence of the pathogens on farm workers' hands could occurred as a result of contact with cattle faecal matter [4]. Therefore, a further study is needed to explain the current findings.…”

Section: Isolation Of E Colimentioning

confidence: 79%

“…Several reports have also suggested some possible critical points of E. coli O157:H7 infection as well as the risk of spread to a wider population. Among of this point are hand of workers in farm and abattoir, water through, irrigation water, as well as fresh fruits and vegetables growing in surrounding area of cattle farm location [2,4,5,6,7]. It means, those critical points need to be closely monitored for any risk of the route of spread of E. coli O157:H7 infection both to animal and human.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre

et al. 2020

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This study was aimed to identify possible critical points of Escherichia coli (E. coli ) O157:H7, a pathogenic agent, contamination in aceh cattle breeding centre. For this purpose, samples were collected from cattle faeces, hand of workers (animal keepers), and water sources in the farm using cross-sectional approach. A number of 85 samples of cattle faecal swab were collected randomly from the animals in the breeding centre. The samples of swab of hand of all workers (15 persons) were collected before and after work. Then, the water sources from 11 cattle house locations in the breeding centre were collected. The water sources were divided into three different locations, namely the water containers, taps, and water puddle on the floors. At each source a number of 11 samples were collected. Isolation of E. coli was conducted on Eosin Methylene Blue Agar (EMBA), followed by identification on Sorbitol MacConkey Agar (SMAC). Then Molecular subtyping of E. coli O157:H7 genes was conducted using multiplex-PCR analysis. Data were analysed descriptively. The results of this study showed that 72 samples (85 %) among 85 samples were positive for E. coli and the rest of samples were positive for other types of bacteria. Sample isolation from swabs of hand was found 3 positive E. coli before work and 1 positive E. coli after work from 15 workers. The most potential water sources for E. coli contamination were the water in taps, and water puddle on the floor of cattle houses. Then, two of samples of E. coli isolated from rectal swab were confirmed as E. coli O157:H7 using PCR test, based on the presence of stx2 gene. In conclusion, the risk of presence of E. coli as zoonotic agents of E. coli O157:H7 in aceh cattle as well as from the farm workers and surrounding area are high. An appropriate control strategy is needed to apply in the aceh cattle farm to prevent from E. coli O157:H7 outbreak in the future.

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Prevalence of Escherichia Coli 0157: H7 and Enterobacteriaceae on Hands of workers in Halal Cattle Abattoirs in Peninsular Malaysia

Cited by 7 publications

References 15 publications

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

Shiga-toxigenic Escherichia coli in ready-to-eat food staffs: Prevalence and distribution of putative virulence factors

Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre

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