Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas

Velandia-Romero, Myriam L.; Coronel-Ruiz, Carolina; Castro-Bonilla, Lorena; Camacho-Ortega, Sigrid; Calderón-Peláez, María-Angélica; Castellanos, Alejandro; Olano, Víctor Alberto; Porras, Alexandra; Arévalo‐Herrera, Myriam; Villamil-Gómez, Wilmer E.; Castellanos, Jaime E.

doi:10.1016/j.ijid.2019.10.045

Cited by 22 publications

(22 citation statements)

References 29 publications

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“…There are few studies where IgG Capture ELISA test has been included as a diagnostic marker and for confirmation of dengue cases [37, 38, 81, 82]. In the present study, we found that the frequency of positive samples in the IgG RDT was higher than that obtained in the IgG Capture ELISA test (66.1% and 52.4%).…”

Section: Discussioncontrasting

confidence: 56%

Improving dengue case confirmation by combining rapid diagnostic test, clinical, and laboratory variables

Coronel-Ruiz

Velandia-Romero

Calvo

et al. 2021

Preprint

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Background Dengue is the most widely distributed arboviral disease in tropical and subtropical countries. Early diagnosis is difficult, and most of the suspected cases are diagnosed according to clinical criteria. In underdeveloped countries, laboratory tests are done on a proportion of dengue with warning signs or severe dengue suspect cases. This study aimed to design a diagnostic algorithm using rapid diagnostic tests (RDT), ELISA tests together with clinical and hematology variables to confirm dengue cases in febrile patients from an endemic area in Colombia. Methods and results. A total of 505 samples were collected from patients with the acute febrile syndrome (<7 days) assisted to the Municipal Hospital in Girardot (Colombia). Serum samples were evaluated by rapid diagnostic tests -RDT- (IgM and IgG antibodies and NS1 antigen immunochromatographic assay), capture ELISAs (IgM, IgG, and NS1 antigen), and by RT-PCR. We analyzed individual test performance to determine which were the most useful to confirm dengue cases. Individual results for IgM, IgG, and NS1 RDT yield low sensitivity and specificity values than the reference standard. A high sensitivity (96.3%) and specificity (96.4%) were obtained after combining the IgM and NS1 ELISAs results. The analysis using the combined NS1 RDT and IgM ELISA results showed 90.3% sensitivity and 96.2% specificity. Adjusted odd ratios (aOR) were calculated including data from symptoms, signs, and diagnostic laboratory tests to differentiate dengue from other febrile illnesses (OFI). Myalgia (aOR: 1.87, CI95%: 1.04-3.38), abdominal tenderness (aOR: 1.89, CI95%: 1.14-3.10), platelets count <140.000/mm3 (aOR: 2.19 CI95%: 1.31-3.67). The analysis using the results of the diagnostic test yields significant ratios for IgM RDT (aOR:2.63 CI95%: 1.59-4.33) and NS1 RDT also differentiate dengue cases from OFI. Combined positive IgM or NS1 RDT and the one that combined positive NS1 RDT or IgM ELISA detect dengue cases in 81.6% and 90.6%, respectively (p<0.001). Conclusion. Our findings showed that only clinical diagnosis does not confirm true dengue cases dengue and needs to be complemented by laboratory diagnostic tests to establish the diagnosis. We also demonstrate the usefulness of rapid tests in diagnosis, suggesting their implementation with IgM ELISA test to better confirm dengue cases.

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Section: Discussioncontrasting

confidence: 56%

Improving dengue case confirmation by combining rapid diagnostic test, clinical, and laboratory variables

Coronel-Ruiz

Velandia-Romero

Calvo

et al. 2021

Preprint

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“…Compared with other dengue-endemic Southeast Asian countries, the seroprevalence rate in this study was higher than that found in Singapore [ 33 , 34 ], while countries such as Thailand, Laos, and the Philippines reported even higher rates [ 35 , 36 ]. Compared to Latin American countries, which are also endemic with dengue, the DENV seroprevalence rate was comparable to two other cities, namely São Paulo, Brazil [ 37 ] and Maracay, Venezuela [ 38 ], but was lower than that of another community study conducted in Columbia [ 39 ].…”

Section: Discussionmentioning

confidence: 71%

Dengue Seroprevalence and Factors Associated with Dengue Seropositivity in Petaling District, Malaysia

Chong

Mutalip

et al. 2022

IJERPH

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Dengue virus (DENV) infection is a major public health concern, posing huge economic and disease burdens globally. In Malaysia, the incidence of DENV infections has increased significantly over the years. Nevertheless, the passive surveillance mechanism applied may not capture the actual magnitude of DENV infection. There was also a paucity of community-based studies exploring DENV seroprevalence. This study aimed to determine the DENV seroprevalence and the associated factors among the urban population in Petaling district, Malaysia. A population-based cross-sectional study was conducted from 18 August to 26 October 2018 with 533 participants recruited. Blood samples were collected and analysed for DENV seropositivity using a composite reference standard comprised of three dengue serological tests. Associated factors were identified by fitting Generalised Linear Mixed Models with binomial error structure and logit link function. DENV seroprevalence obtained was 79.0% (95% CI: 75.2–82.4%). The age-specific DENV seroprevalence showed an increasing trend with advancing age, from 22.7% (95% CI: 9.6–45.0%) for those aged below five years old to 94.9% (95% CI: 81.3–98.7%) for those aged ≥60 years old. Only age group and house level were found to be significant factors associated with DENV seropositivity. The odds of being DENV seropositive generally increased with age, from 13.43 (95% CI: 2.77–65.22) for the 5–9 years old age group to 384.77 (95% CI: 39.27–3769.97) for the ≥60 years old age group, as compared to those aged below 5 years old. For house level, those who lived on the first and second floor (OR: 8.98, 95% CI: 3.16–25.12) and the third floor and above (OR: 4.82, 95% CI: 1.89–12.32) had greater odds of being DENV seropositive compared to those living on the ground floor. This study demonstrated the persistently high DENV seroprevalence among the urban population in Petaling district, which could be useful to evaluate dengue control measures taken and estimate more accurate disease incidence. The associated factors with DENV seropositivity identified could also contribute to undertaking more targeted preventive and control measures.

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“…Predominance of the dengue virus by serotypes in Colombia: Predominance rate by serotypes in Colombia by year (A); The predominance of dengue virus serotypes by departments in different years (B)[14,15,[22][23][24]28,[35][36][37].…”

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confidence: 99%

Dengue Infections in Colombia: Epidemiological Trends of a Hyperendemic Country

Gutiérrez-Barbosa

Medina‐Moreno²,

Zapata³

et al. 2020

TropicalMed

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Dengue is a major public health problem in hyperendemic countries like Colombia, the understanding of the epidemiological trends is important for the development of efficient public health policies. We conducted a systematic review of the epidemiologic data on dengue in Colombia from 1971 to 2020. A total of 375 relevant citations were identified, 36 of which fulfilled the inclusion criteria. The data of dengue and severe dengue cases, infection fatality rate, and serotype distribution were used to understand and identify gaps in the epidemiological knowledge in Colombia. The epidemiology of dengue in this country was characterized by five main outbreaks in 1998, 2002, 2010, 2013, and 2019 with high fatality rates in comparison with the average values reported in the Americas. The case fatality rate of severe dengue exceeded 2% and all four serotypes co-circulate throughout the country with some regional variations. Overall, the behavior of dengue in Colombia is influenced by multiple factors including seasonal temperature variation and socioeconomic conditions. Additionally, the most important barriers in the epidemiological surveillance of dengue may be due to the insufficient notification rate in some regions and the low active search for the circulation of different serotypes.

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Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas

Cited by 22 publications

References 29 publications

Improving dengue case confirmation by combining rapid diagnostic test, clinical, and laboratory variables

Improving dengue case confirmation by combining rapid diagnostic test, clinical, and laboratory variables

Dengue Seroprevalence and Factors Associated with Dengue Seropositivity in Petaling District, Malaysia

Dengue Infections in Colombia: Epidemiological Trends of a Hyperendemic Country

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