Prevalence of Cryptosporidium sp. In Patients with Colorectal Cancer

Sulżyc-Bielicka, Violetta; Kołodziejczyk, Lidia; Jaczewska, S.; Bielicki, Dariusz; Kładny, Józef; Safranow, Krzysztof

doi:10.2478/v10035-012-0058-4

Cited by 37 publications

(23 citation statements)

References 18 publications

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“…Several epidemiological studies demonstrated that colorectal cancer is highly correlated with Cryptosporidium spp. [10][11][12][13][14]. In consistence, we here found that 17.24% patients with colorectal cancer had C. parvum infections, which was significantly higher than control population.…”

Section: Discussionsupporting

confidence: 49%

“…In 2007, the first epidemiological study in Poland showed that 10 out of 55 (18%) patients with diagnosed colorectal cancer had cryptosporidiosis, and the infection rate was even higher in those patients with diarrhea symptom (43.5%, 10/55) [10]. Another two reports also conducted in Poland showed similar infection rates (12.6%, 11/87 and 13%, 14/108) [11,12]. Recently, Osman M. et al (2017) performed a systematic analysis among Lebanese patients which indicated that Cryptosporidium spp.…”

Section: Ivyspringmentioning

confidence: 96%

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Prevalence and Genotyping of Cryptosporidium parvum in Gastrointestinal Cancer Patients

Zhang

et al. 2020

J. Cancer

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Gastrointestinal cancers are the most commonly occurring malignancies which contributing to over 1/5 of cancer incidences worldwide. Increasing evidences have shown that Cryptosporidium spp., an apicomplexan protozoan, is highly associated with gastrointestinal cancers. However, the prevalence of Cryptosporidium spp. infections among gastrointestinal cancer patients in China has not been estimated yet. We here performed a case-control study to evaluate the occurrences of Cryptosporidium spp. in patients with digestive malignancies before chemotherapy and in control population. Nested PCR amplifying 18S rRNA gene was used to detect the presence of Cryptosporidium spp. in each fecal sample. The results herein confirmed the correlation of Cryptosporidium spp. infection with colorectal and liver cancers, while first identified the high frequencies of Cryptosporidium spp. in esophageal cancer and small intestine cancer. The infection rates of Cryptosporidium spp. in colorectal, esophageal, liver and small intestine cancers were 17.24% (20/116, P<0.001), 6.25% (1/16, P=0.029), 14.29% (1/7, P<0.001) and 40% (2/5, P<0.001), respectively. In addition, molecular characterization indicated that all the Cryptosporidium spp. obtained were Cryptosporidium parvum (C. parvum), and the 18S rRNA sequences were identical to the reference sequences isolated from cattle, suggesting potential zoonotic transmission. Furthermore, subtyping analyses revealed that IIaA15G2R1 and IIaA15G2R2 were the predominant subtypes in colorectal cancer, while IIaA13G2R2 subtype was first named and identified in colorectal and liver cancers. Taken together, for the first time, the prevalence of Cryptosporidium spp. infections in digestive cancer patients has been estimated among Chinese. Our results indicated that C. parvum were highly associated with gastrointestinal cancers, supporting that cryptosporidiosis could be a potential risk factor for these diseases.

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Section: Discussionsupporting

confidence: 49%

Section: Ivyspringmentioning

confidence: 96%

Prevalence and Genotyping of Cryptosporidium parvum in Gastrointestinal Cancer Patients

Zhang

et al. 2020

J. Cancer

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“…A study reported that colon squamous cell carcinoma risk was significantly elevated among AIDS patients who had cryptosporidiosis [25]. Furthermore, a recent epidemiological study in Poland reported a frequency of 12.6% of cryptosporidiosis in patients with colorectal cancer [26].…”

Section: Discussionmentioning

confidence: 99%

Cryptosporidium parvum Infection in SCID Mice Infected with Only One Oocyst: qPCR Assessment of Parasite Replication in Tissues and Development of Digestive Cancer

et al. 2012

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Dexamethasone (Dex) treated Severe Combined Immunodeficiency (SCID) mice were previously described as developing digestive adenocarcinoma after massive infection with Cryptosporidium parvum as soon as 45 days post-infection (P.I.). We aimed to determine the minimum number of oocysts capable of inducing infection and thereby gastrointestinal tumors in this model. Mice were challenged with calibrated oocyst suspensions containing intended doses of: 1, 10, 100 or 105 oocysts of C. parvum Iowa strain. All administered doses were infective for animals but increasing the oocyst challenge lead to an increase in mice infectivity (P = 0.01). Oocyst shedding was detected at 7 days P.I. after inoculation with more than 10 oocysts, and after 15 days in mice challenged with one oocyst. In groups challenged with lower inocula, parasite growth phase was significantly higher (P = 0.005) compared to mice inoculated with higher doses. After 45 days P.I. all groups of mice had a mean of oocyst shedding superior to 10,000 oocyst/g of feces. The most impressive observation of this study was the demonstration that C. parvum-induced digestive adenocarcinoma could be caused by infection with low doses of Cryptosporidium, even with only one oocyst: in mice inoculated with low doses, neoplastic lesions were detected as early as 45 days P.I. both in the stomach and ileo-caecal region, and these lesions could evolve in an invasive adenocarcinoma. These findings show a great amplification effect of parasites in mouse tissues after challenge with low doses as confirmed by quantitative PCR. The ability of C. parvum to infect mice with one oocyst and to develop digestive adenocarcinoma suggests that other mammalian species including humans could be also susceptible to this process, especially when they are severely immunocompromised.

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“…Immunosuppression and diarrhoea are well-recognised side-effects of cancer treatment, yet relatively few studies have been conducted examining the prevalence of Cryptosporidium in cancer patients (Botero et al, 2003;Tamer et al, 2008;Al-Qobati et al, 2012;Hassanein et al, 2012;Sulżyc-Bielicka et al, 2012;García-Elorriaga et al, 2013). In Jordan, the molecular epidemiology of cryptosporidiosis is poorly understood and to date, only two genotyping studies have been conducted (Hijjawi et al, 2010;Hijjawi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Cryptosporidium species and subtypes in paediatric oncology and non-oncology patients with diarrhoea in Jordan

Hijjawi

Zahedi

Kazaleh

et al. 2017

Infection, Genetics and Evolution

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Cryptosporidiosis is a protozoan parasitic disease which affects human and animals worldwide. In adult immunocompetent individuals, cryptosporidiosis usually results in acute and self-limited diarrhoea; however, it can cause life threatening diarrhoea in children and immunocompromised individuals. In the present study, we compared the prevalence of Cryptosporidium species and gp60 subtypes amongst paediatric oncology patients with diarrhoea (n=160) from King Hussein Medical Centre for Cancer in Jordan, and non-oncology paediatric patients with diarrhoea (n=137) from Al-Mafraq paediatric hospital. Microscopy results using modified acid fast staining identified a significantly (p≤0.05) higher prevalence of Cryptosporidium in paediatric oncology patients with diarrhoea (14.4% - 23/160), compared to non-oncology paediatric patients with diarrhoea only (5.1% - 7/137). With the exception of one sample, all microscopy-positive samples (n=29) and an additional 3/30 microscopy-negative controls were typed to species and subtype level at the 18S and gp60 loci, respectively. All Cryptosporidium positives were typed as C. parvum. Of the 22 typed Cryptosporidium positives from the paediatric oncology patients, 21 were subtyped as IIaA17G2R1 and one as IIaA16G2R1 C. parvum subtypes. The 7 typed positives from the paediatric patients from Al-Mafraq hospital were subtyped as IIaA17G2R1 (n=5) and IIaA16G2R1 (n=2). The 3 additional positives from the 30 microscopy negative control samples were subtyped as IIaA17G2R1. The high prevalence of the IIaA17G2R1 subtype, particularly amongst oncology patients, suggests that an outbreak of cryptosporidiosis may have been occurring in oncology patients during the collection period (April to December, 2016). New therapies for cryptosporidiosis in immunocompromised patients are urgently required.

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Prevalence of Cryptosporidium sp. In Patients with Colorectal Cancer

Cited by 37 publications

References 18 publications

Prevalence and Genotyping of Cryptosporidium parvum in Gastrointestinal Cancer Patients

Prevalence and Genotyping of Cryptosporidium parvum in Gastrointestinal Cancer Patients

Cryptosporidium parvum Infection in SCID Mice Infected with Only One Oocyst: qPCR Assessment of Parasite Replication in Tissues and Development of Digestive Cancer

Prevalence of Cryptosporidium species and subtypes in paediatric oncology and non-oncology patients with diarrhoea in Jordan

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