Prevalence of Cryptosporidium infections in pigs in Aragón (northeastern Spain)

Quílez, Joaquín; Sánchez-Acedo, Caridad; Clavel, Antonio; Cacho, Emilio del; López-Bernad, F.

doi:10.1016/s0304-4017(96)01026-6

Cited by 51 publications

(46 citation statements)

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“…Natural cryptosporidiosis in pigs has then been reported worldwide (Xiao et al 1994;Quílez et al 1996;Izumiyama et al 2001;Wieler et al 2001;Maddox-Hyttel et al 2006;Vítovec et al 2006;Xiao et al 2006;Langkjaer et al 2007;Suárez-Luengas et al 2007;Zintl et al 2007). Pig cryptosporidiosis can be caused by three distinct species/genotypes of Cryptosporidium: Cryptosporidium parvum, Cryptosporidium suis, or Cryptosporidium pig genotype II.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

et al. 2008

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A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial small subunit ribosomal RNA (SSU rRNA) and GP60 partial genes and polymerase chain reaction restriction fragment length polymorphism of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and two sows (10%). Twenty-one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and Cryptosporidium suis in finishers were found. Both sows were infected with the Cryptosporidium parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

et al. 2008

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“…Fluorescence microscopy and direct fluorescent-antibody (DFA) assay have been used with relatively high specificities (96 to 100%) and sensitivities (98.5 to 100%) for the detection of Cryptosporidium oocysts in clinical and environmental samples (139,158,209). However, the sensitivity and specificity of monoclonal antibody (MAb)-based DFA assays have been affected by different factors, such as the purity of the Cryptosporidium antigen originally used to raise the MAb (139,158,209,214,329).…”

Section: Cryptosporidium Speciesmentioning

confidence: 99%

“…Staining and preservation methods have been used to enhance the sensitivity of tests. Unfortunately, these do not identify species, and their analytical sensitivity can be poor, especially for samples containing small numbers of oocysts (138,253,329). The modified ZiehlNeelsen technique (413) is used widely; however, one recent study found that it was less sensitive than PCR (75.7%; 95% CI ϭ 68.3 to 81.8%) but was highly specific (100%; 95% CI ϭ 96.5 to 100%) (56).…”

Section: Cryptosporidium Speciesmentioning

confidence: 99%

“…Antigen detection by immunoassays has been used widely in the diagnosis of cryptosporidiosis, as these assays are thought to be more sensitive than conventional staining and more effective in cases where oocyst numbers are low (139,141,329 (140). A multicenter French study evaluated the sensitivity and specificity of four immunochromatographic (ICT) assays and found that RIDAQuick (mean sensitivity, 73.3%), Remel Xpect (mean sensitivity, 74.1%), and ImmunoCard STAT (mean sensitivity, 73.3%) were fairly sensitive for detecting C. parvum and C. hominis but were very limited in detecting other Cryptosporidium spp.…”

Section: Cryptosporidium Speciesmentioning

confidence: 99%

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Enteric Protozoa in the Developed World: a Public Health Perspective

et al. 2012

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SUMMARY Several enteric protozoa cause severe morbidity and mortality in both humans and animals worldwide. In developed settings, enteric protozoa are often ignored as a cause of diarrheal illness due to better hygiene conditions, and as such, very little effort is used toward laboratory diagnosis. Although these protozoa contribute to the high burden of infectious diseases, estimates of their true prevalence are sometimes affected by the lack of sensitive diagnostic techniques to detect them in clinical and environmental specimens. Despite recent advances in the epidemiology, molecular biology, and treatment of protozoan illnesses, gaps in knowledge still exist, requiring further research. There is evidence that climate-related changes will contribute to their burden due to displacement of ecosystems and human and animal populations, increases in atmospheric temperature, flooding and other environmental conditions suitable for transmission, and the need for the reuse of alternative water sources to meet growing population needs. This review discusses the common enteric protozoa from a public health perspective, highlighting their epidemiology, modes of transmission, prevention, and control. It also discusses the potential impact of climate changes on their epidemiology and the issues surrounding waterborne transmission and suggests a multidisciplinary approach to their prevention and control.

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“…The role of dogs in the transmission of C. parvum is controversial, but Abe et al (2002) denied the possibility of dogs transmission by using PCR. Atwill et al(1997) suggested that feral pigs may serve as an environmental reservoir of C. parvum, and Quilez et al (1996) detected C. parvum oocysts from 21.9% of pigs. Izumiyama et al (2001) observed that weaned piglets excreted more oocysts than older pigs, suggesting weaned piglets are important reservoirs of C. parvum by contaminating drinking water.…”

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confidence: 99%

Infection status of pigs with Cryptosporidium parvum

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2004

Korean J Parasitol

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Prevalence of Cryptosporidium infections in pigs in Aragón (northeastern Spain)

Cited by 51 publications

References 15 publications

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

Enteric Protozoa in the Developed World: a Public Health Perspective

Infection status of pigs with Cryptosporidium parvum

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