Prevalence of Chlamydia trachomatis, Chlamydia psittaci and Chlamydia pneumoniae antibodies in blood donors and attendees of STD clinics

Bergström, Kristina; Domeika, Marius; Vaitkienė, Daiva; Persson, Kenneth; Mårdh, Per‐Anders

doi:10.1016/s1198-743x(15)60284-1

Cited by 13 publications

(11 citation statements)

References 31 publications

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“…The test detects both group-specific lipopolysaccharide and species-specific antibodies [13]. Therefore, women with a positive serology but with a normal pelvis may have cross-reactive responses to past infection with other species of chlamydia such as Chlamydia pneumoniae [14] or Chlamydia psittaci [12,15]. In our study we did not evaluate C. pneumoniae antibodies.…”

Section: Discussionmentioning

confidence: 99%

High Titers of Chlamydia trachomatis Antibodies in Brazilian Women with Tubal Occlusion or Previous Ectopic Pregnancy

Machado

Guimarães

Sakurai

et al. 2007

Infectious Diseases in Obstetrics and Gynecology

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Objective. To evaluate serum chlamydia antibody titers (CATs) in tubal occlusion or previous ectopic pregnancy and the associated risk factors.Methods. The study population consisted of 55 women wih tubal damage and 55 parous women. CAT was measured using the whole-cell inclusion immunofluorescence test and cervical chlamydial DNA detected by PCR. Odds ratios were calculated to assess variables associated withC. trachomatis infection.Results. The prevalence of chlamydial antibodies and antibody titers in women with tubal occlusion or previous ectopic pregnancy was significantly higher (P < .01) than in parous women. Stepwise logistic regression analysis showed that chlamydia IgG antibodies were associated with tubal damage and with a larger number of lifetime sexual partners.Conclusions. Chlamydia antibody titers were associated with tubal occlusion, prior ectopic pregnancy, and with sexual behavior, suggesting that a chlamydia infection was the major contributor to the tubal damage in these women.

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Section: Discussionmentioning

confidence: 99%

High Titers of Chlamydia trachomatis Antibodies in Brazilian Women with Tubal Occlusion or Previous Ectopic Pregnancy

Machado

Guimarães

Sakurai

et al. 2007

Infectious Diseases in Obstetrics and Gynecology

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“…Sera from patients exposed to C. trachomatis show diverse serological profiles against immunodominant C. trachomatis antigens (2,18,42,47,49), many of which are cross-reactive with sera from patients exposed to other chlamydial species, in particular C. pneumoniae (2,5,19,44). In addition, C. trachomatis antigens, such as the 60-kDa heat shock protein (hsp60) and lipopolysaccharide (LPS), cross-react with other bacterial species (35,50,57).…”

mentioning

confidence: 99%

“…Microimmunofluorescence (MIF) (54), which detects antibodies to chlamydial elementary bodies (EB), has long been considered the "gold standard" for the serodiagnosis of chlamydial infections (55). However, the procedure lacks standardization and is subjective; moreover, its specificity is considered suspect because of cross-reactivity with other chlamydial species (5,27,40,44). A number of ELISAs are also commercially available, including several based on peptides of MOMP, which makes up 60% of the total outer membrane protein and is highly immunogenic (8).…”

mentioning

confidence: 99%

Pgp3 Antibody Enzyme-Linked Immunosorbent Assay, a Sensitive and Specific Assay for Seroepidemiological Analysis of Chlamydia trachomatis Infection

Wills

Horner

Reynolds

et al. 2009

Clin Vaccine Immunol

135

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Understanding of the burden of Chlamydia trachomatis infection and its clinical sequelae is hampered by the absence of accurate, well-characterized tests using serological methods to determine past exposure to infection. An "in-house" immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) based on the C. trachomatis-specific antigen Pgp3 was produced and evaluated against three commercial ELISAs derived from the major outer membrane protein: the Medac pELISA plus, the Savyon SeroCT-IgG ELISA, and the Ani Labsystems IgG enzyme immunoassay. Sensitivities and specificities were determined using sera from both male and female patients (n ‫؍‬ 356) for whom C. trachomatis had been detected in the lower genital tract at least 1 month prior to the testing of the sample and from 722 Chlamydia-negative children aged 2 to 13 years. Chlamydia trachomatis is the commonest sexually transmitted bacterial infection in developed countries, with national surveillance programs consistently showing rising rates of diagnosed infections over the past decade. In the United Kingdom, figures based on cases diagnosed in departments of genitourinary medicine (GUM) suggest a population rate of 190 per 100,000 men and 187 per 100,000 women (52). Reported rates are highly dependent on the level of testing at different clinics, with the probability that many Chlamydia cases are not diagnosed. The population prevalence of uncomplicated genital Chlamydia in 16-to 24-year-olds in the United Kingdom is thought to be between 2% and 6% in both men and women (17,33), while the opportunistic National Chlamydia Screening Programme (2008) indicates a higher prevalence of around 10%, likely due to selective testing of higher-risk individuals (15). Nucleic acid amplification tests, commonly used in GUM clinics, identify infection only when the organism is present. Once infection has been resolved, these tests provide no information on past exposure. While detection rates are rising, due in part to increased screening and testing, the overall prevalence of past C. trachomatis exposure is not known.The prevalence of past exposure to genital C. trachomatis and changes over time in age-specific prevalence can be explored serologically. For instance, in Finland, Lyytikäinen et al. (32) studied pregnant women under the age of 29 using a commercial enzyme-linked immunosorbent assay (ELISA) based on C. trachomatis-specific peptides derived from the major outer membrane protein (MOMP). However, for wider application, confidence in the sensitivity and specificity of available antibody tests is critical (24). None of the current ELISAs have ever been rigorously evaluated against large numbers of well-defined serologically positive and negative control sera; hence, their sensitivity and specificity remain open to question.Chlamydia trachomatis is from the same family, Chlamydiaceae, as Chlamydia pneumoniae, a common respiratory pathogen with which it shares genetic homology (26). Sera from patients exposed to C. trachomatis show diverse serological

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“…Thirty‐nine serum samples from patients with diagnosed SARA were selected on the basis of the results of the MIF test [18], i.e. meeting requirements for groups 1 and 2 (see below).…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study [18]we found that antibody reactivity to all three chlamydial species occurred more often in patients with urethritis, assumed salpingitis, fertility problems and sexually acquired reactive arthritis (SARA) than in blood donors. This was also true for those who were direct immunofluorescence (DIF)‐positive, but not for those in whom no elementary bodies (EBs) were found.…”

Section: Introductionmentioning

confidence: 96%

Characterization of serum antibody response to chlamydiae in patients with sexually acquired reactive arthritis

Domeika

Brade

Mårdh

et al. 2006

FEMS Immunology &Amp; Medical Microbiology

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Sera from patients with sexually acquired reactive arthritis (SARA) with antibodies reacting with C. trachomatis and C. pneumoniae (group 1; n = 20) and also with C. psittaci (group 2; n = 19) were analyzed for antibody specificity. Sera from group 2 reacted significantly more often with C. trachomatis serotype E, H and K and had higher antibody titers to serotype E, as tested by microimmunofluorescence tests. Cross-reactivities occurring in microimmunofluorescence tests were related to the presence of antichlamydial lipopolysaccharide antibodies, adsorption of which by recombinant lipopolysaccharide removed microimmunofluorescence reactivity with C. psittaci antigen. In group 2, significantly more sera had antibodies to C. pneumoniae, remaining after lipopolysaccharide adsorption, as proved by adsorption with viable C. trachomatis and C. pneumoniae organisms. None of the sera had antibodies to Yersinia enterocolitica, Shigella flexneri, Sh. sonnei and Salmonella spp. It was observed that the frequency and titer of cross-reacting antibodies to chlamydial serotypes and species were related to the time period between the diagnosis of genital chlamydial infection and of SARA. Cross-reactivities were also related to the presence of lipopolysaccharide, but not heat shock protein 60- or neutralizing antibodies to chlamydiae. Antibody reactivity induced by antichlamydial lipopolysaccharide antibodies can be removed by lipopolysaccharide adsorption.

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Prevalence of Chlamydia trachomatis, Chlamydia psittaci and Chlamydia pneumoniae antibodies in blood donors and attendees of STD clinics

Cited by 13 publications

References 31 publications

High Titers of Chlamydia trachomatis Antibodies in Brazilian Women with Tubal Occlusion or Previous Ectopic Pregnancy

High Titers of Chlamydia trachomatis Antibodies in Brazilian Women with Tubal Occlusion or Previous Ectopic Pregnancy

Pgp3 Antibody Enzyme-Linked Immunosorbent Assay, a Sensitive and Specific Assay for Seroepidemiological Analysis of Chlamydia trachomatis Infection

Characterization of serum antibody response to chlamydiae in patients with sexually acquired reactive arthritis

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