Prevalence of Cefotaxime-Resistant Escherichia coli Isolates from Healthy Cattle and Sheep in Northern Spain: Phenotypic and Genome-Based Characterization of Antimicrobial Susceptibility

Tello, Maitane; Ocejo, Medelin; Oporto, Beatriz; Hurtado, Ana

doi:10.1128/aem.00742-20

Cited by 22 publications

(31 citation statements)

References 59 publications

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“…In conclusion, our study found a low incidence of AmpC-producing E. coli (11/78 enrichments from 7/26 farms) compared with European studies [14, 15, 37, 47], and no ESBL- or plasmid-mediated AmpC-producing E. coli were detected.…”

Section: Discussioncontrasting

confidence: 42%

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The epidemiology of AmpC-producing Escherichia coli isolated from dairy cattle faeces on pasture-fed farms

Burgess

Brousse

Ortolani

et al. 2021

Journal of Medical Microbiology

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Introduction. Antibiotic use, particularly amoxicillin-clavulanic acid in dairy farming, has been associated with an increased incidence of AmpC-hyperproducing Escherichia coli . Gap statement. There is limited information on the incidence of AmpC-hyperproducing E. coli from seasonal pasture-fed dairy farms. Aim. We undertook a New Zealand wide cross-sectional study to determine the prevalence of AmpC-producing E. coli carried by dairy cattle. Methodology. Paddock faeces were sampled from twenty-six dairy farms and were processed for the selective growth of both extended-spectrum beta-lactamase (ESBL)- and AmpC-producing E. coli . Whole genome sequence analysis was carried out on 35 AmpC-producing E. coli . Results. No ESBL- or plasmid mediated AmpC-producing E. coli were detected, but seven farms were positive for chromosomal mediated AmpC-hyperproducing E. coli . These seven farms were associated with a higher usage of injectable amoxicillin antibiotics. Whole genome sequence analysis of the AmpC-producing E. coli demonstrated that the same strain (<3 SNPs difference) of E. coli ST5729 was shared between cows on a single farm. Similarly, the same strain (≤15 SNPs difference) of E. coli ST8977 was shared across two farms (separated by approximately 425 km). Conclusion. These results infer that both cow-to-cow and farm-to-farm transmission of AmpC-producing E. coli has occurred.

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Section: Discussioncontrasting

confidence: 42%

“…In previous cross-sectional studies carried out in Europe, of those E. coli isolates with an AmpC phenotype, 18–20 % harboured a mutation in the ampC promoter and the remainder carried the bla CMY-2 or bla CMY-4 gene [15, 37]. The mutations found in the promoter and attenuator regions of the E.…”

Section: Discussionmentioning

confidence: 99%

The epidemiology of AmpC-producing Escherichia coli isolated from dairy cattle faeces on pasture-fed farms

Burgess

Brousse

Ortolani

et al. 2021

Journal of Medical Microbiology

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“…Overall, in the eight EU countries supplying data on resistance in E. coli from calves to ampicillin, cefotaxime, ciprofloxacin and tetracycline for the period 2009-2017, there were 10 decreasing and 8 increasing trends [7]. A study in Spanish cattle herds tested E. coli isolated from healthy animals of all ages and found a significantly higher prevalence of resistance in dairy herds than in beef herds [26]. As much as 97.8% of all isolates were resistant to fourth-generation cephalosporins, 87.4% were resistant to third-generation cephalosporins, 70.4% to tetracycline, 70.4% to sulfamethoxazole, 47.4% to trimethoprim, 41.5% to ciprofloxacin, 28.9% to chloramphenicol and 23.7% to gentamicin.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Patterns in Organic and Conventional Dairy Herds in Sweden

et al. 2020

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Monitoring antimicrobial resistance (AMR) and use (AMU) is important for control. We used Escherichia coli from healthy young calves as an indicator to evaluate whether AMR patterns differ between Swedish organic and conventional dairy herds and whether the patterns could be related to AMU data. Samples were taken twice, in 30 organic and 30 conventional dairy herds. Selective culturing for Escherichia coli, without antibiotics and with nalidixic acid or tetracycline, was used to estimate the proportions of resistant isolates. Microdilution was used to determine the minimum inhibitory concentrations (MICs) for thirteen antimicrobial substances. AMU data were based on collection of empty drug packages. Less than 8% of the bacterial growth on non-selective plates was also found on selective plates with tetracycline, and 1% on plates with nalidixic acid. Despite some MIC variations, resistance patterns were largely similar in both periods, and between organic and conventional herds. For most substances, only a few isolates were classified as resistant. The most common resistances were against ampicillin, streptomycin, sulfamethoxazole, and tetracycline. No clear association with AMU could be found. The lack of difference between organic and conventional herds is likely due to a generally good animal health status and consequent low AMU in both categories.

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“…To the best of our knowledge, only a handful of studies have reported the use of carbapenem agars as an initial screening step with the objective of detecting CP-producing GN bacteria in the gut of healthy cattle ( 11 , 52 ) or in milk from healthy cattle ( 53 ). In our study, screening with commercial carbapenem agars led to the isolation of mainly Pseudomonas spp., Ochrobactrum spp.…”

Section: Discussionmentioning

confidence: 99%

Surveillance of Extended-Spectrum β-Lactamase-, Cephalosporinase- and Carbapenemase-Producing Gram-Negative Bacteria in Raw Milk Filters and Healthy Dairy Cattle in Three Farms in Île-de-France, France

et al. 2021

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The aim of this work was to test a surveillance protocol able to detect extended-spectrum β-lactamase (ESBL)-, cephalosporinase (AmpC)- and carbapenemase (CP)-producing gram-negative bacteria in three conveniently chosen dairy farms with known prior occurrences of ESBL- and CP-producing strains. The protocol was applied monthly for a year. At each visit, 10 healthy lactating dairy cows were rectally swabbed, and raw milk filters (RMFs) were sampled in two of the three farms. Bacterial isolation was based on a first screening step with MacConkey agar supplemented with 1 mg/L cefotaxime and commercial carbapenem-supplemented media. We failed to detect CP-producing strains but showed that ESBL-Escherichia strains, found in one farm only (13 strains), were closely associated with multi-drug resistance (12 out of 13). The limited number of conveniently selected farms and the fact that RMFs could not be retrieved from one of them limit the validity of our findings. Still, our results illustrate that ESBL-status changes monthly based on fecal swabs and negative herds should be qualified as “unsuspected” as proposed by previous authors. Although surveillance of farm statuses based on RMF analysis could theoretically allow for a better sensitivity than individual swabs, we failed to illustrate it as both farms where RMFs could be retrieved were constantly negative. Determination of CP herd-level status based on RMFs and our surveillance protocol was hindered by the presence of intrinsically resistant bacteria or strains cumulating multiple non-CP resistance mechanisms which means our protocol is not specific enough for routine monitoring of CP in dairy farms.

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Prevalence of Cefotaxime-Resistant Escherichia coli Isolates from Healthy Cattle and Sheep in Northern Spain: Phenotypic and Genome-Based Characterization of Antimicrobial Susceptibility

Cited by 22 publications

References 59 publications

The epidemiology of AmpC-producing Escherichia coli isolated from dairy cattle faeces on pasture-fed farms

The epidemiology of AmpC-producing Escherichia coli isolated from dairy cattle faeces on pasture-fed farms

Antimicrobial Resistance Patterns in Organic and Conventional Dairy Herds in Sweden

Surveillance of Extended-Spectrum β-Lactamase-, Cephalosporinase- and Carbapenemase-Producing Gram-Negative Bacteria in Raw Milk Filters and Healthy Dairy Cattle in Three Farms in Île-de-France, France

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