Prevalence of capsular polysaccharide types 5 and 8 among Staphylococcus aureus isolates from cow, goat, and ewe milk

Poutrel, B.; Boutonnier, A; Sutra, L.; Fournier, J.M.

doi:10.1128/jcm.26.1.38-40.1988

Cited by 96 publications

(71 citation statements)

References 14 publications

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“…In contrast, type 5 and 8 strains are the predominant isolates which account for approx. 80% of recent isolates from all sources (Albus et al, 1988;Arbeit et al, 1984;Hochkeppel et al, 1987;Karakawa and Vann, 1982;Karakawa et al, 1985;Poutrel et al, 1988;Sompolinsky et al, 1985). These strains produce a small amount of capsule and are therefore referred to as 'microencapsulated' (Wilkinson, 1983). S. aureus M, isolated from an infected human-hand laceration, is a type 1 capsule producer (Scott, 1969).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of type 1 capsule genes in Staphylococcus aureus

Ouyang

Lee

1997

Molecular Microbiology

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SummarySerotype 1 capsule of Staphylococcus aureus plays an important role in staphylococcal infections. A 14.6 kb chromosomal DNA fragment containing 13 cap1 genes responsible for the synthesis of type 1 capsule in S. aureus strain M has been previously cloned and sequenced in our laboratory. These genes are closely linked and are transcribed in one orientation. To study the transcription of these genes, we employed genetic complementation using a set of plasmids with various deletions in the 14.6 kb region to complement mutants mapped in each of the 13 genes. We found that there were six transcriptional units. By Northern hybridization using the entire gene cluster as a probe, we identified a large 14 kb band and several smaller bands ranging from 0.3 to 6 kb. The 14 kb band was also identified by various individual gene probes, suggesting that all 13 genes are transcribed into a large transcript. The 14 kb transcript and the smaller bands were not detected when a 533 bp fragment containing the potential promoter region upstream of the first gene (cap1A) was deleted from the chromosome, suggesting that the small transcripts are the degradation (processing) products of the 14 kb transcript. The activities of the promoters identified by genetic complementation tests were quantitatively measured by transcriptional fusions using xylE as the reporter gene. The result showed that the promoter preceding the first cap1 gene was 45-198-fold higher than the downstream promoters. The result from gene-fusion studies is in agreement with the Northern analysis in that only the transcript transcribed from the first promoter was detected. Taken together, these results suggest that the cap1 genes are organized as a large operon with at least five weak internal promoters, and the long polycistronic transcript is processed (degraded) into several smaller transcripts. The transcription initiation site of the primary transcript was mapped by S1 nuclease mapping and the major promoter was defined by deletion analysis of the promoter-xylE fusion. In addition, we showed that the internal promoters, despite their weak activities, were sufficiently effective at expressing their downstream genes required for producing capsule, compared to the wild-type strain.

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Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of type 1 capsule genes in Staphylococcus aureus

Ouyang

Lee

1997

Molecular Microbiology

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“…The presence of capsular polysaccharides in vitro and in vivo (POUTREL et al 1988;JOHNE et a]., 1989;SUTRA et al, 1990) and pseudocapsules (WATSON and FRANKLIN, 1988) has been reported. Although the relationship between these surface structures and those expressed in serum-soft media is not fully understood (SUTRA et al, ), JOHNE et al (1989 found a correlation between presence of exopolysaccharides in vivo and D C M in Staphylococcus medium 110 (agar 1.5 "A) in S. aureus cultured directly from milk.…”

Section: Discussionmentioning

confidence: 99%

“…Distinct surface components have been detected in Staphylococcus aureus isolated from bovine milk using tests that showed a good correlation with encapsulation (YOKOMIZO et al, 1977;POUTREL et al, 1988;WATSON and FRANKLIN, 1988). These structures have been defined as capsules (YOKOMIZO et al, 1977;OPDEBEECK and NORCROSS, 1983), microcapsules (SOMPOLINSKY et al, 1985) and pseudocapsules (WATSON and FRANKLIN, 1988) and have been typed using different serological methods.…”

Section: Introductionmentioning

confidence: 99%

Virulence for Mice of Staphylococcus aureus Strains from Bovine Mastitis Related to Colonial Morphology and Serological Types in Serum‐soft Agar

Calvinho

1994

Journal of Veterinary Medicine, Series B

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S u m m a r y Six Staphylococcus aureus strains isolated from bovine mastitic milk representing the typical growth types in soft agar media were injected into mice via the intraperitoneal route. Strains showing diffuse colony morphology (DCM) in serum-soft agar (SSA) as a permanent characteristic which reacted against anti-capsular sera types A, B, and D were virulent for mice. A strain showing DCM in SSA that reacted only against anti-capsular serum D behaved as a compact-colony-morphology-type strain in the peritoneal cavity of the mouse. Diffuse-type colony morphology and presence of capsular antigens type A, B, and D correlated with increased virulence for mice, but a capsule could not be demonstrated.

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“…However, in the case of S. aureus causing bovine mastitis, the expression of a polysaccharide capsule has long been debated and different definitions have been suggested by several investigators. Poutrel et al (12) reported that S. aureus strains isolated from cow, goat and ewe (4) reported that S. aureus strains isolated from ruminant mastitis produce a loose exopolysaccharide structure in vivo defined as slime. After a similar study, Sutra et al (15) also reported that the majority of S. aureus strains isolated from the milk of cows with mastitis tested reacted with capsular type 5 and 8 antibodies.…”

Section: Staphylococcusmentioning

confidence: 99%

In Vivo‐Like Antigenic Surface Properties of Staphylococcus aureus from Bovine Mastitis Induced upon Growth in Milk Whey

Mamo

Fröman

1994

Microbiology and Immunology

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Antigenic surface properties of Staphylococcus aureus strains grown in milk whey were compared with TSB-grown bacteria using immuno-gold electron microscopy. It is shown that colloidal gold (CG) particles coated with polyclonal antibody raised against Staphylococcus aureus surface antigen expressed in vivo bound to the surface of S. aureus strain F1440 grown in milk whey, but not to homologous bacteria grown in TSB. S. aureus strains grown in milk whey agglutinated in the presence of the polyclonal antibody, whereas the corresponding bacteria grown in TSB did not agglutinate. Immuno-gold particles did not bind to milk whey-grown bacteria treated with periodate. Periodate-treated milk whey-grown bacteria did not agglutinate in the presence of the polyclonal antibody, whereas periodate treatment had no effect on TSB-grown bacteria.

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Prevalence of capsular polysaccharide types 5 and 8 among Staphylococcus aureus isolates from cow, goat, and ewe milk

Cited by 96 publications

References 14 publications

Transcriptional analysis of type 1 capsule genes in Staphylococcus aureus

Transcriptional analysis of type 1 capsule genes in Staphylococcus aureus

Virulence for Mice of Staphylococcus aureus Strains from Bovine Mastitis Related to Colonial Morphology and Serological Types in Serum‐soft Agar

In Vivo‐Like Antigenic Surface Properties of Staphylococcus aureus from Bovine Mastitis Induced upon Growth in Milk Whey

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