Virulence for Mice of <i>Staphylococcus aureus</i> Strains from Bovine Mastitis Related to Colonial Morphology and Serological Types in Serum‐soft Agar

Calvinho, Luis Fernando

doi:10.1111/j.1439-0450.1994.tb00235.x

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…The nBt protein was partially purified from a culture supernatant of a S. aureus strain isolated from a bovine mastitis case and characterized to produce only nBt in vitro (Calvinho and Dodd, 1994). Hemolytic activity against sheep erythgrocytes was assessed by pre-incubating the nBt with 2-fold serially diluted serum samples from vaccinated heifers.…”

Section: Antibody Determinationmentioning

confidence: 99%

Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers

Pujato

Camussone

Renna

et al. 2018

The Veterinary Journal

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Section: Antibody Determinationmentioning

confidence: 99%

Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers

Pujato

Camussone

Renna

et al. 2018

The Veterinary Journal

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“…of 5×10 8 C.F.U/ ml. The injected mice were kept under observation for 7 days and the results of mortality and resolution were recorded according to Calvinho and Dodd (1994).…”

Section: Pathogenicitymentioning

confidence: 99%

Some Bacteriological, Hematological and Biochemical Changes on Clinical Mastitis in Goat

2010

Assiut Veterinary Medical Journal

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This study included clinical examination of 120 goats bred in different localities of Dakahlia Governorate where 70% were clinically mastitic. The results of microscopical examination of 168 mastitic milk samples revealed that 73.8% were positive, 77.4% out of them gave pure single cultures and 22.6% yielded mixed ones. The bacteriological examination of positive samples yielded 124 isolates where Staphylococcus aureus, Staph. epidermidis, E. coli, Streptococcus agalactiae, Strept. dysglactiae and Strept. uberis were the main organisms. These strains were isolated at varying percentages 35.4%, 27.1%, 16.1%, 10.4%, 8.3% and 2.1% respectively. Pathogenicity test in mice revealed that Staph. aureus and E. coli were highly virulent strains. The results of the invitro antimicrobial sensitivity test showed that Enrofloxacin, Ceftiofur, Ciprofloxacin, Gentamycin and Lincomycin were the most effective antibiotics for successful treatment of mastitic goats. Biochemical studies of blood serum samples of mastitic does were discussed in details and revealed on increase in the levels of total protein, Alpha 2, gamma globulin, but the level of albumin and A/G ratio were decreased significantly in their values. Meanwhile studies of minerals profiles revealed that, the mean values of Ca, IP, Mg, K, Zn and Fe were decreased significantly, at the same time the level of Na and Cu values were increased significantly. Some biochemical liver and kidney functions in the present studies were discussed and revealed a significant increased in the liver enzymes AST, ALT and also in the level of blood urea nitrogen and serum creatinine. Hematological parameters of blood samples of mastitis does were also discussed in details and the studies revealed that, the mean values of Hb, TRBCs, TWBCs were decreased significantly. At the same time, the differential leucocytic count profiles revealed that the percentage of lymphocytes, neutropbils were decreased significantly, while the percentage of monocytes, eosinophils and basophiles were significantly increased.

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“…Group one and two were injected intraperitonialy with 0.5ml of 5x10 9 C.F.U/ ml of S. aureus and S. aureus subsp. anaerobius isolates respectively according to (Calvinho and Dodd, 1994;Nevine, 2001), the control mice were injected with sterile saline.…”

mentioning

confidence: 99%

Studies on Corynebacterium pseudotuberculosis, Staphylococcus aureus and Staphylococcus aureus subsp. anaerobius isolated from sheep skin abscesses in Beni Suef Governorate.

Afifi¹

2010

Journal of Veterinary Medical Research

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Virulence for Mice of Staphylococcus aureus Strains from Bovine Mastitis Related to Colonial Morphology and Serological Types in Serum‐soft Agar

Cited by 3 publications

References 27 publications

Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers

Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers

Some Bacteriological, Hematological and Biochemical Changes on Clinical Mastitis in Goat

Studies on Corynebacterium pseudotuberculosis, Staphylococcus aureus and Staphylococcus aureus subsp. anaerobius isolated from sheep skin abscesses in Beni Suef Governorate.

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