Prevalence of Blood Group A2 in Northern Pakistan

Yazdani, Muhammad Sajid; Khalid, Zeeshan; Rathore, Muhammad Ali; Fatima, Sarah

doi:10.51253/pafmj.v72i1.3912

Cited by 2 publications

(3 citation statements)

References 7 publications

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“…This difference may be due to ethnicity and higher ratio of cousin marriages in those areas. 8 Previous studies done in India showed almost same results with 95.9% of A1 antigen (subgroup A1) and 4.1% had no detectable A1 antigen (subgroup A2) and among AB group individuals 80.8% had A1 antigen (subgroup A1B) and 67(19.2%) had no detectable A1 antigen (subgroup A2B). 12 , 13 Study done in South India also showed similar results.…”

Section: Discussionmentioning

confidence: 54%

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Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors

Khanum,

Farhan,

Saqlain

et al. 2023

Pak J Med Sci

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Objective: To determine the frequency of A2 and A2B subgroups among blood groups A and AB in healthy donors. Methods: It was a Cross-Sectional study, conducted at the Department of Hematology & Transfusion Medicine, UCHS, The Children’s Hospital Lahore and Sundas foundation Lahore from June 2022 to December 2022 including 13,120 healthy blood donors of both genders, after taking informed consent. Venous blood samples of donors were collected in EDTA vials (3ml) and serum gel vial for routine blood grouping which was done by standard tube method. Further testing of donors positive for an antigen (blood Group-A and AB) was performed using anti-A1 lectin by standard tube method as per manufacturer’s instruction. The data was analyzed using SPSS version 23. Results: Among 13120 blood donors, 12857 (97.9%) were male and 263 (2.0%) were female with mean age of 36.7 years ± 15.04 years. Majority of them (91.7%) were of Punjabi ethnicity. Donors having blood group phenotype A and AB were 3890 (29.6%). Among blood Group-A donors, A1 was found in 97.8% and A2 in 2.2% donors. While among Blood Group-AB, 96.7% donors belonged to A1B blood group and 3.2% belonged to A2B blood group. Conclusions: Blood group A2 and A2B do exist in blood donors of Punjabi ethnicity. The knowledge of presence of these blood groups’ phenotypes in our population can provide a better base for transfusion staff to do troubleshooting in compatibility testing and to avoid any rare but hazardous transfusion outcome. doi: https://doi.org/10.12669/pjms.40.1.7916 How to cite this: Khanum A, Farhan S, Saqlain N, Arshad S. Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors. Pak J Med Sci. 2024;40(1):---------. doi: https://doi.org/10.12669/pjms.40.1.7916 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 54%

“…However, studies were done in Northern Pakistan recently with the same objective. 8 So, the present study will help to establish the prevalence of A2 subgroup in our region of the province Punjab, which will help to establish the need of A2 testing as a routine blood banking procedure.…”

Section: Introductionmentioning

confidence: 85%

Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors

Khanum,

Farhan,

Saqlain

et al. 2023

Pak J Med Sci

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“…A study conducted in northern Pakistan has documented similar results showing the frequency of Blood Groups A1 and A2 being 21.6% and 3.2%. 13 The prevalence of Blood Group A1 and A2 in Rayalaseema (India) is approximately 95.8% and 4.1% with anti-A1 lectin, respectively. 14 study from west India has described similar results showing the prevalence of A2 on anti-A1 typing to be around 3.0%.…”

Section: Discussionmentioning

confidence: 99%

Serological and Molecular Characterization of Blood Group A2 in Pakistan

Habib¹,

Salamat²,

Din³

et al. 2023

PAFMJ

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Objective: To determine the frequency of Blood Group A2 genotype among Group A Pakistani whole blood donors. Study Design: Cross-sectional study. Place and Duration of Study: Armed Forces Institute of Transfusion (AFIT), Rawalpindi Pakistan, from Jan 2019 to Jan 2020. Methodology: One thousand (1000) healthy and unrelated blood donors were selected. The blood samples were typed for ABO, and those of Blood Groups-A and AB were further subtyped with the help of anti-A1 lectin to categorize them as A1,non-A1, A1B and non-A1B Groups. Next, DNA of non-A1 samples was extracted, and a Polymerase Chain Reaction using sequence-specific primers (PCR-SSP) for type A2 was performed, followed by polyacrylamide gel electrophoresis (PAGE). Results: Among one thousand blood donors, 247(24.7%) were typed as Blood Group-A, 94(9.4%) as AB-Group, 339(33.9%) BGroup and 320(32%) O Group with variable strength of reaction with ABO antisera. A and AB Blood Groups were further subgrouped as A1 202(20.2%), A1B 77(7.7%), non-A1 45(4.5%) and non-A1B 17(1.7%). Anti-A1 antibodies were detected in 6(13.3%) of non-A1 samples. PCR of non-A1 samples showed 32(12.9%) to be genotypically A2, and the remaining 13(5.2%) were not A2 and were not further resolved. Conclusion: Blood Group A2 is not a rare Subgroup in our population. PCR-SSP is a more specific technique than anti-A1 lectin for establishing the Blood Group status of an individual.

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Prevalence of Blood Group A2 in Northern Pakistan

Cited by 2 publications

References 7 publications

Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors

Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors

Serological and Molecular Characterization of Blood Group A2 in Pakistan

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