Serological and Molecular Characterization of Blood Group A2 in Pakistan

Habib, Iram; Salamat, Nuzhat; Din, Nasir Ud; Yazdani, Muhammad Sajid; Khan, Saleem Ahmed; Naeem, Muhammad Abdul

doi:10.51253/pafmj.v73i1.6143

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…3 Another study was done in Rawalpindi Pakistan in which molecular testing of these blood groups was done and according to genotype of blood groups A1 and A2 is 81.7% and 12.9% respectively. 15 Severe transfusion reactions can occur due to these minor incompatibilities. Implementation of testing of A1 and A2 sub-groups in routine ABO typing is vital for limiting the preventable transfusion related adverse events ensuring recipients' safety.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors

Khanum,

Farhan,

Saqlain

et al. 2023

Pak J Med Sci

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Objective: To determine the frequency of A2 and A2B subgroups among blood groups A and AB in healthy donors. Methods: It was a Cross-Sectional study, conducted at the Department of Hematology & Transfusion Medicine, UCHS, The Children’s Hospital Lahore and Sundas foundation Lahore from June 2022 to December 2022 including 13,120 healthy blood donors of both genders, after taking informed consent. Venous blood samples of donors were collected in EDTA vials (3ml) and serum gel vial for routine blood grouping which was done by standard tube method. Further testing of donors positive for an antigen (blood Group-A and AB) was performed using anti-A1 lectin by standard tube method as per manufacturer’s instruction. The data was analyzed using SPSS version 23. Results: Among 13120 blood donors, 12857 (97.9%) were male and 263 (2.0%) were female with mean age of 36.7 years ± 15.04 years. Majority of them (91.7%) were of Punjabi ethnicity. Donors having blood group phenotype A and AB were 3890 (29.6%). Among blood Group-A donors, A1 was found in 97.8% and A2 in 2.2% donors. While among Blood Group-AB, 96.7% donors belonged to A1B blood group and 3.2% belonged to A2B blood group. Conclusions: Blood group A2 and A2B do exist in blood donors of Punjabi ethnicity. The knowledge of presence of these blood groups’ phenotypes in our population can provide a better base for transfusion staff to do troubleshooting in compatibility testing and to avoid any rare but hazardous transfusion outcome. doi: https://doi.org/10.12669/pjms.40.1.7916 How to cite this: Khanum A, Farhan S, Saqlain N, Arshad S. Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors. Pak J Med Sci. 2024;40(1):---------. doi: https://doi.org/10.12669/pjms.40.1.7916 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%

Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors

Khanum,

Farhan,

Saqlain

et al. 2023

Pak J Med Sci

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“…We can differentiate the red blood cells based on antigens present on their surfaces. The glycosyltransferase gene is responsible for the expression of ABO antigens on their surfaces [2,3]. Thus, blood group A contains only A antigens, B contains only B antigens, AB contains both A and B antigens, O does not have A or B antigens and Rh gives information about Rh antigens [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Selective Detection of Erythrocytes with QCMs—ABO Blood Group Typing

Latif,

Seifner,

Dickert

2023

Sensors

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Blood transfusion, as well as organ transplantation, is only possible after prior blood group (BG) typing and crossmatching. The most important blood group system is that of Landsteiner’s ABO classification based on antigen presence on the erythrocyte surfaces. A mass sensitive QCM (quartz crystal microbalance) sensor for BG typing has been developed by utilizing molecular imprinting technology. Polyvinylpyrrolidone (crosslinked with N,N-methylenebisacrylamide) is a favorable coating that was imprinted with erythrocytes of different blood groups. In total, 10 MHz quartz sheets with two resonators, one for MIP (molecularly imprinted polymer) and the other for NIP (non-imprinted polymer) were fabricated and later used for mass-sensitive measurements. The structure of erythrocyte imprints resembles a donut, as identified by AFM (atomic force microscope). All the erythrocytes of the ABO system were chosen as templates and the responses to these selective coatings were evaluated against all blood groups. Each blood group can be characterized by the pattern of responses in an unambiguous way. The results for blood group O are remarkable given that all types of erythrocytes give nearly the same result. This can be easily understood as blood group O does not possess neither antigen A nor antigen B. The responses can be roughly related to the number of respective antigens on the erythrocyte surface. The imprints generate hollows, which are used for reversible recognition of the erythrocytes. This procedure is based on molecular recognition (based on supramolecular strategies), which results from size, shape and enthalpic interactions between host and guest molecules.

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Serological and Molecular Characterization of Blood Group A2 in Pakistan

Cited by 2 publications

References 15 publications

Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors

Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors

Selective Detection of Erythrocytes with QCMs—ABO Blood Group Typing

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