Prevalence of Biofilms and Their Response to Medical Treatment in Chronic Rhinosinusitis without Polyps

Tatar, Emel Çadallı; Tatar, İlkan; Öcal, Bülent; Korkmaz, Hakan; Saylam, Güleser; Özdek, Ali; Çelik, H. Hamdi

doi:10.1177/0194599811434101

Cited by 33 publications

(23 citation statements)

References 29 publications

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“…There is no univerTurkiye Klinikleri J Med Sci 2013;33 (6) sally accepted grading system, but we used a grading system which was used in the previous studies of one of our authors. [7][8][9] Analysis of the data was done using SPSS (SPSS for Windows, version 11.5, SPSS, Chicago, IL). Continuous variables were expressed as mean ± standard deviation (SD) and categorical variables were expressed as percentages.…”

Section: Methodsmentioning

confidence: 99%

Presence of Biofilms in the Lacrimal Sac Mucosa

Koşarsoy¹,

Özer²,

Öǧretmenoǧlu³

et al. 2013

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : The inflammation and the following fibrosis due to the bacterial colonization are thought to be the principle underlying mechanism in the development of nasolacrimal duct stenosis. The aim of this study was to investigate the presence of biofilms in the lacrimal sac mucosa of the patients with dacryostenosis. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : This study included 15 patients with a symptom of epiphora for at least 3 months and documented dacryostenosis with a dacryocystography. Lacrimal sac mucosal specimens obtained during endoscopic dacryocystorhinostomy (DCR) were examined for the presence of biofilm formation under the scanning electron microscope. R Re es su ul lt ts s: : The specimens were obtained from 15 patients. The mean age of 12 female and 3 male patients was 54 years. Epiphora was the main symptom and its average duration was 18 (6-36) months. We could not analyze specimen in 1 patient. We detected biofilm formation in 12 of 14 (% 85.7) specimens. Biofilm formation was dense in 5 specimens and light in 7 specimens. C Co on nc cl lu us si io on n: : This is the first article that documents the presence of bacterial biofilms on the lacrimal sac mucosa in the patients with dacryostenosis. This study shows the presence of biofilms in cases with dacryostenosis and interrogates its role in the etiology of chronic dacryocystitis similar to other chronic infections. Further investigations on this subject should be carried on in larger and controlled series to assure the role of biofilms in the etiology of chronic infections. New studies should also investigate whether the biofilm formation is the reason or the result of chronic infections. We believe that, control of biofilm formation in chronic infections would prevent secondary problems like the development of dacryostenosis.K Ke ey y W Wo or rd ds s: : Biofilms; dacryocystitis; microscopy, electron, scanning; dacryocystorhinostomy Ö ÖZ ZE ET T A Am ma aç ç: : Bakteriyel kolonizasyon sonucu gelişen inflamasyon ve fibrozis, nazolakrimal kanal stenozuna yol açan temel mekanizma olarak kabul edilmektedir. Bu çalışmanın amacı, dakriostenozlu olguların lakrimal kese mukozalarında biofilm varlığını araştırmaktır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Bu çalışmaya en az 3 aydır epifora şikayeti olan ve dakriosistografide dakriostenoz tespit edilen 15 olgu dahil edilmiştir. Endoskopik dakriosistorinostomi sırasında elde edilen lakrimal kese mukoza örnekleri, tarayıcı elektron mikroskopide incelenerek biyofilm varlığı araştırılmıştır. B Bu ul lg gu ul la ar r: : Ortalama yaşı 54 olan 12 kadın ve 3 erkek, toplam 15 hasta çalışmaya dahil edilmiştir. Hastalarda görülen en sık semptom epifora idi ve ortalama 18 aydır mevcuttu. İncelenen 14 spesmenin 12 (%85,7)'sinde biyofilm tespit edildi. Spesmenlerin 1 tanesinin analizi yapılamadı. Biyofilm formasyonu örneklerin 5'inde hafif, 7'sinde ise yoğun idi. S So on nu uç ç: : Bu makale dakriostenozlu hastaların lakrimal kese muko...

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Section: Methodsmentioning

confidence: 99%

Presence of Biofilms in the Lacrimal Sac Mucosa

Koşarsoy¹,

Özer²,

Öǧretmenoǧlu³

et al. 2013

Turkiye Klinikleri J Med Sci

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“…Chen and colleagues used SEM only (no culture methods) to examine surgical specimens from 24 patients, observing biofi lms on the mucosal surfaces of 13 specimens which were more commonly from revision surgeries (Chen et al 2012a ). Likewise, Tatar and colleagues performed a clinical trial of clarithromycin with or without mometasone treatment for 8 weeks in 32 chronic rhinosinusitis patients, obtaining surgical specimens before and after therapy in each group (Tatar et al 2012 ). Biofi lms were found in 75 % of specimens before therapy, and 44 % of specimens after therapy.…”

Section: Biofi Lms From Aerobic Grampositive and Gram-negative Bacteriamentioning

confidence: 99%

Biofilm Formation by Clinical Isolates and Its Relevance to Clinical Infections

Akers

Cardile

Wenke

et al. 2014

Advances in Experimental Medicine and Biology

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Reports of biofilms have increased exponentially in the scientific literature over the past two decades, yet the vast majority of these are basic science investigations with limited clinical relevance. Biofilm studies involving clinical isolates are most often surveys of isolate collections, but suffer from lack of standardization in methodologies for producing and assessing biofilms. In contrast, more informative clinical studies correlating biofilm formation to patient data have infrequently been reported. In this chapter, biofilm surveys of clinical isolates of aerobic and anaerobic bacteria, mycobacteria, and Candida are reviewed, as well as those pertaining to the unique situation of cystic fibrosis. In addition, the influence of host components on in vitro biofilm formation, as well as published studies documenting the clinical impact of biofilms in human infections, are presented.

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“…In theory it would be sufficient to clean the sinuses to allow their contents to drain and to remove all the contaminants from the nasal cavity to allow natural healing. Recent findings clearly prove that bacterial biofilm which obstructs sinus openings in as many as 75% CRS patients is the main cause of persistent CRS [6,23]. A biofilm is a three-dimensionally structured specialized community of adherent microorganisms surrounded by an extracellular polymeric substance (EPS), a strong bodyguard for hidden microorganisms.…”

Section: Discussionmentioning

confidence: 99%

“…A few patients of this study complained about moderate nasal irritation right after each TP application, lasting for about [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] seconds. This may be attributable to the fact that FPG solution is osmotic and draws intracellular hypotonic liquid as soon as it is sprayed as a film over the nasal mucosa.…”

Section: Discussionmentioning

confidence: 99%

“…It should be noted that cleaning the nasal sinuses is the prerequisite to treat CRS yet up till now there was no mechanical, chemical or physiological treatment capable of providing nearly instant relief although several efforts have been made (application of Nacetylcysteine, antibiotics, steroids and sea water saline with betamethasone) but without success [23,[32][33][34][35][36]. Therefore, the discovery of FPG, a new generation of mechanically acting filmogen, cell-friendly, non-irritant, topically applicable solution devoid of side effects which can be used as an osmotic bandage on the nasal mucosa should be considered as a major breakthrough for the treatment of CRS.…”

Section: Discussionmentioning

confidence: 99%

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Clinical Efficacy of a New Filmogen Polymeric Glycerol Solution for the Treatment of Rhinosinusitis

Rm¹,

Tourret²,

Schütte³

et al. 2017

J Clin Respir Dis Care

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Study background: The main cause of chronic rhinosinusitis is not yet well understood but increasing amount of evidence suggest an initial viral attack followed by secondary bacterial infection leading to the formation of a wellprotected reservoir of microorganisms in the sinuses where no treatment can easily reach. The efficacy of a new, non-irritant and moderately osmotic, polymeric glycerol filmogen solution was evaluated clinically.Methods: A 14-day, randomized, placebo-controlled, double blind, efficacy and safety clinical study was conducted with participation of thirty-eight patients treated with the filmogen polymeric glycerol vs. saline solution as comparator treatment used on sixteen control patients. Two to three nasal sprays were applied, three times daily over maximum fourteen days. Effects on rhinorrhea, nasal congestion, headache, facial pain, and sino-nasal outcome test were evaluated.Results: Rhinorrhea was much stronger in the test product group during the 1st three days compared to comparator and decreased drastically thereafter. Severity of all other rhinosinusitis symptoms also diminished in both groups but the reduction was much faster and stronger in the test group compared to comparator group (p<0.05), and led to minimized requirement for antibiotics. The test product was non-irritant and totally safe, no adverse effects being observed. Conclusion:Treating rhinosinusitis with a moderately osmotic solution capable of generating positive osmotic pressure yet non-irritant polymeric film constitutes a major breakthrough in the treatment of Rhinosinusitis.

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Prevalence of Biofilms and Their Response to Medical Treatment in Chronic Rhinosinusitis without Polyps

Abstract: The prevalence of biofilms in CRS without polyps was 75% in our study. Regression of biofilms to 43% was observed under medical treatment. Adding nasal steroids to macrolides gave no further benefit.

Cited by 33 publications

References 29 publications

Presence of Biofilms in the Lacrimal Sac Mucosa

Presence of Biofilms in the Lacrimal Sac Mucosa

Biofilm Formation by Clinical Isolates and Its Relevance to Clinical Infections

Clinical Efficacy of a New Filmogen Polymeric Glycerol Solution for the Treatment of Rhinosinusitis

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