Prevalence of antinuclear autoantibodies in the serum of normal blood dornors

Fernandez, Solange Assuncion Villagra; Lobo, Alice Z.C.; Oliveira, Zilda Najjar Prado de; Fukumori, Ligia Maria Ichimura; Périgo, Alexandre M.; Rivitti, Evandro A.

doi:10.1590/s0041-87812003000600005

Cited by 70 publications

(62 citation statements)

References 6 publications

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“…RA is in general not an ANA-associated disease, since ANA positivity has been reported in only up to 30% of patients with RA [26]. Despite the fact that the prevalence of ANAs has been reported to be higher in healthy women than that in healthy men [27, 28] and higher in elderly than in younger healthy individuals [29, 30], in the ILD subjects of our study we did not observe these associations.…”

Section: Discussioncontrasting

confidence: 79%

Utility of Anti-DSF70 Antibodies to Predict Connective Tissue Disease in Patients Originally Presenting with Idiopathic Interstitial Pneumonia

et al. 2019

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Background: Anti-DFS70 antibodies, corresponding to the dense fine speckled antinuclear antibody (ANA) pattern in HEp-2 substrates, have been observed in chronic inflammatory conditions, cancer and in healthy individuals but in only a small percentage of patients with connective tissue diseases (CTD). Objectives: The study was aimed to investigate the possible role of Anti-DFS70 antibodies to distinguish CTD associated interstitial lung disease (CTD-ILD) from idiopathic interstitial pneumonia (IIP) and to explore potential correlations between anti-DFS70 antibodies and clinical parameters. Methods: Serum samples were collected from 49 healthy controls (HC), 35 scleroderma-ILD (SSc-ILD) patients as negative controls for anti-DFS70 antibody, and 260 patients with the initial diagnosis IIP including 100 nonspecific interstitial pneumonia (NSIP) and 160 idiopathic pulmonary fibrosis (IPF) patients. ANA pattern was identified by indirect immunofluorescence on HEp-2 cells and anti-DFS70 antibodies were measured in serum by ELISA. Results: Serum anti-DFS70 antibodies were less frequently seen in ILD and SSc-ILD patients compared to HCs. Thirty-seven patients (34 initial idiopathic NSIP and 3 initial IPF patients) developed CTD during 24 months of follow-up, most of them combined with ANA positivity and anti-DFS70 antibody negativity. Anti-DFS70 antibody positivity was not significantly different between CTD-ILD and idiopathic ILD. Conclusions: The frequency of serum anti-DFS70 antibody is markedly decreased in patients with ILDs. Anti-DFS70 antibodies may be useful to predict CTD development in ILD patients.

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Section: Discussioncontrasting

confidence: 79%

Utility of Anti-DSF70 Antibodies to Predict Connective Tissue Disease in Patients Originally Presenting with Idiopathic Interstitial Pneumonia

et al. 2019

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“…No entanto, o teste de FAN deve ser complementado pela pesquisa e identificação de auto-anticorpos e auto-antígenos específicos, muitos dos quais apresentam grande utilidade clínica (4) . Apesar de FAN positivo ser um dos 11 critérios estabelecidos pelo Colégio Americano de Reumatologia para a classificação de lúpus eritematoso sistêmico (LES) (5) , sua presença não necessariamente indica um estado patológi-co. Tal situação foi demonstrada em um estudo feito em doadores de banco de sangue, em que 22,6% (113/500) dos doadores apresentavam FAN positivo e 20,4% destes com título de 1:80 (6) . A pesquisa de antígenos nucleares extraíveis (ENA) é usada para identificação de um grupo de auto-anticorpos específicos que inclui o anti-SSA/Ro, anti-SSB/La, anti-RNP, anti-Sm, anti-Scl-70 e anti-Jo-1.…”

Section: Introductionunclassified

“…Priscila Schmidt Lora (1) , Claudia Cilene Fernandes Correia Laurino (2) , Adriana Estigarribia de Freitas (3) , João Carlos T. Brenol (4) , Odirlei Montecielo (5) , Ricardo Machado Xavier (6) RESUMO Objetivo: avaliar os padrões de imunofluorescência do fator antinuclear (FAN) em soros reagentes para anticorpos anti-SSA/Ro e sua associação clínica. Método: foi realizado um estudo transversal retrospectivo, no qual foram revisadas as solicitações de anticorpos antiantígenos nucleares extraíveis (anti-ENA) encaminhadas ao SPC/HCPA no período de dois anos.…”

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Padrões de imunofluorescência do fator antinuclear (FAN) em células HEp-2 de soros reagentes para anti-SSA/Ro

Lora¹,

Laurino²,

Freitas³

et al. 2007

Rev. Bras. Reumatol.

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RESUMOObjetivo: avaliar os padrões de imunofluorescência do fator antinuclear (FAN) em soros reagentes para anticorpos anti-SSA/Ro e sua associação clínica. Método: foi realizado um estudo transversal retrospectivo, no qual foram revisadas as solicitações de anticorpos antiantígenos nucleares extraíveis (anti-ENA) encaminhadas ao SPC/HCPA no período de dois anos. Das solicitações com resultado positivo para anti-ENA identificou-se qual ou quais auto-anticorpos estavam envolvidos (anti-SSA/Ro, anti-SSB/La, anti-RNP, anti-Sm, anti-Scl-70), bem como os padrões de imunofluorescência do FAN e os quadros clínicos dos pacientes anti-SSA/Ro positivo. As técnicas usadas para detecção e identificação foram FAN por imunofluorescência indireta (IFI) em células HEp-2 e anti-ENA por hemaglutinação. Resultados: das 392 solicitações analisadas 90 eram anti-ENA positivo. Houve um predomínio do sexo feminino (94%) (86/91) e a idade média foi de 42 anos. O anti-SSA/Ro foi o mais freqüente (67,8%) (61/90), sendo que todas as amostras anti-SSA/Ro positivas eram positivas para o FAN. O padrão de imunofluorescência nuclear pontilhado fino foi o predominante (68,9%) (42/61) nos pacientes com anti-SSA/Ro positivo, e o quadro clínico mais encontrado foi de lúpus eritematoso sistêmico, em 50,8% (31/61) dos pacientes. Conclusão: o teste de FAN por IFI utilizando células HEp-2 é um bom método de triagem para detecção de auto-anticorpos anti-SSA/Ro, apresentando maior associação com o padrão nuclear pontilhado fino. Diferente do que tem sido descrito na literatura, não encontramos nenhuma amostra de pacientes com anti-SSA/Ro que tenham apresentado FAN falso-negativo na IFI. Pelo menos na nossa experiência, esses dados questionam o custo-efetividade da solicitação de rotina desse exame em pacientes FAN negativo pelo teste de IFI.Palavras-chave: auto-anticorpo, fator antinuclear, anticorpos anti-SSA/Ro, anti-ENA. ABSTRACTObjective: to evaluate the pattern at immunofluorescence of the antinuclear antibodies (ANA) detected by the indirect immunofluorescence (IIF) technique in positive samples for anti-SSA/Ro autoantibody and the clinical associations. Methods: a retrospective transversal study was performed in a period of two years where the all the solicitations of testing for the presence of anti-extractable nuclear antigen (anti-ENA) antibodies delivered to the SPC/HCPA were analyzed. We selected the positive samples and identified which autoantibodies were involved (anti-SSA/RO, anti-SSB/La, anti-RNP, anti-Sm and anti-Scl70) as well as the immunofluorescence patterns by ANA testing and the clinical associations found in the patients presenting anti-SSA/Ro positive serum. IIF was used for ANA using HEp-2 cells and hemagglutination for anti-ENA antibodies detection. Results: 90 out of the 392 solicitations analyzed were anti-ENA positive, with a predominance of women (86/91 -94%) and the mean age was 42 years old. The most frequent autoantibody was anti-SSA/Ro (61/90 -67.8%) and all samples that were anti-SSA/Ro positive were also ANA positive...

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“…Investigation of ANA seroprevalences is important, not only in the identification of autoimmune diseases in societies, but also in revealing subclinical conditions prior to disease, guiding early treatment and monitoring and in establishing the risk factors for these diseases (2). ANA seroprevalences between 4.2% and 22.6% have been reported for different societies (3)(4)(5)(6)(7)(8)(9). However, there are insufficient data regarding the ANA seroprevalence for the Eastern Black Sea region of Turkey, which includes the province of Trabzon, as well as for other regions of the country.…”

mentioning

confidence: 99%

Determination of Anti-Nuclear Antibody Seroprevalence in Adult Age Groups in Trabzon Province

Kaklıkkaya¹,

Akıneden²,

Topbaş³

et al. 2013

Balkan Med J

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Prevalence of antinuclear autoantibodies in the serum of normal blood dornors

Abstract: The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.

Cited by 70 publications

References 6 publications

Utility of Anti-DSF70 Antibodies to Predict Connective Tissue Disease in Patients Originally Presenting with Idiopathic Interstitial Pneumonia

Utility of Anti-DSF70 Antibodies to Predict Connective Tissue Disease in Patients Originally Presenting with Idiopathic Interstitial Pneumonia

Padrões de imunofluorescência do fator antinuclear (FAN) em células HEp-2 de soros reagentes para anti-SSA/Ro

Determination of Anti-Nuclear Antibody Seroprevalence in Adult Age Groups in Trabzon Province

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