Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic Escherichia coli Recovered from Hospital Food

Ranjbar, Reza; Seif, Ali; Dehkordi, Farhad Safarpoor

doi:10.5812/jjm.82659

Cited by 15 publications

(19 citation statements)

References 46 publications

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“…Furthermore, it can highlight the role of poultry meat in transmission of antibiotic resistance to human population. Presence of virulent and antibiotic resistant O157 bacteria in diverse kinds of food samples, particularly ruminants and poultry meat and their products have also been conveyed in different surveys [1][2][3][4][5][6][7][8][9][10]12]. Concurrent atten dance of ehlyA, stx2, stx1, and eaeAvirulence markers have also been conveyed in the E. coli bacteria isolated from diverse kinds of food samples in Nigeria [21], United States [22] and Iraq [23].…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial isolation was performed rendering the protocols labeled beforeh and [11,12]. For this goal, 25 g of samples were normalized well and one of the achieved solution was blended with 5 mL of buffered peptone water (Merck, Germany).…”

Section: E Coli O157 Isolation and Identificationmentioning

confidence: 99%

“…Definitive detection of O157 serogroup was performed using the Latex agglutination examination in sorbitol negative bacteroid [11]. Diverse biochemical tests such as indole, methylred, Voges-Proskauer and citrate (IMVC) and Triple Sugar Iron Agar (TSIS) were also applied for identification of bacteria [12].…”

Section: E Coli O157 Isolation and Identificationmentioning

confidence: 99%

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Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

2020

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E scherichia coli (E. coli) O157 issubstantial foodborne pathogen. The existing survey was addressed to assess the incidence, phenotypic resistance profile and incidence of virulence markers amongst the E. coli O157 isolates recovered from poultry meat. Five-hundred poultry meat samples were collected from Isfahan, Iran.Poultrymeatsamples were cultured and phenotypic antibiotic resistance pattern was deliberated using disk diffusion. PCR was applied to identify the incidence of virulence markers. Forty-four out of 500 (8.80%) poultry meat samples were contaminated with E. coli O157. Duck meat (16%) harbored the uppermost incidence of E. coli O157. Incidence of ehlyA, stx2, stx1and eaeA were 100%, 27.27%, 100% and 100%, respectively. E. coli O157 bacteria exhibited the maximum incidence of resistance against ampicillin (95.45%), tetracycline (88.63%), gentamycin (84.09%)and trimethoprim (38.63%) antibiotics. Incidence of resistance toward imipenem and chloramphenicol were 6.81% and 27.27%, respectively. Poultry meat samples, particularly duck, were considered as reservoir of virulence and antibiotic resistant O157 bacteria. Thoughtful antibiotic prescription and courtesies to the ideologies of food security can condense the hazard of resistant and virulent E. coli O157 in poultry meat.

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Section: Discussionmentioning

confidence: 99%

Section: E Coli O157 Isolation and Identificationmentioning

confidence: 99%

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Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

2020

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“…Absolutely, impact of food-borne microbes, particularly bacteria, in occurrence of food-borne diseases has been measured in Iran and diverse surveys have been conducted in this field. [57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74]…”

Section: Combined Genotyping Patterns Distribution* (%)mentioning

confidence: 99%

Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of vacA, cagA, iceA, oipA, cagE, and babA2 Alleles of Helicobacter pylori Bacteria Isolated from Raw Meat

Mashak¹,

Jafariaskari²,

Alavi³

et al. 2020

IDR

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Background: Foodstuffs with animal origins, particularly meat, are likely reservoirs of Helicobacter pylori. Purpose: An existing survey was accompanied to assess phenotypic and genotypic profiles of antibiotic resistance and genotyping of vacA, cagA, cagE, iceA, oipA, and babA2 alleles amongst the H. pylori bacteria recovered from raw meat. Methods: Six-hundred raw meat samples were collected and cultured. H. pylori isolates were tested using disk diffusion and PCR identification of antibiotic resistance genes and genotyping. Results: Fifty-two out of 600 (8.66%) raw meat samples were contaminated with H. pylori. Raw ovine meat (13.07%) had the uppermost contamination. H. pylori bacteria displayed the uppermost incidence of resistance toward tetracycline (82.69%), erythromycin (80.76%), trimethoprim (65.38%), levofloxacin (63.46%), and amoxicillin (63.46%). All H. pylori bacteria had at least resistance toward one antibiotic, even though incidence of resistance toward more than eight antibiotics was 28.84%. Total distribution of rdxA, pbp1A, gyrA, and cla antibiotic resistance genes were 59.61%, 51.92%, 69.23%, and 65.38%, respectively. VacA s1a (84.61%), s2 (76.92%), m1a (50%), m2 (39.13%), iceA1 (38.46%), and cagA (55.76%) were the most generally perceived alleles. S1am1a (63.46%), s2m1a (53.84%), s1am2 (51.92%), and s2m2 (42.30%) were the most generally perceived genotyping patterns. Frequency of cagA-, oipA-, and babA2-genotypes were 44.23%, 73.07%, and 80.76%, respectively. A total of 196 combined genotyping patterns were also perceived. Conclusion: The role of raw meat, particularly ovine meat, in transmission of virulent and resistant H. pylori bacteria was determined. VacA and cagA genotypes had the higher incidence. CagE-, babA2-, and oipA-H. pylori bacteria had the higher distribution. Supplementary surveys are compulsory to originate momentous relations between distribution of genotypes, antibiotic resistance, and antibiotic resistance genes.

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“…For instance, septicemia causing E. coli (SCEC) and neonatal meningitis producing E. coli (NMEC) are ExPEC pathotypes as the main cause of septicemia and pediatric meningitis respectively. Another important member of EXPEC pathotypes is uropathogenic E. coli (UPEC) which causes urinary tract infections (UTIs) [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Multilocus Variable Number of Tandem Repeat Analysis for molecular typing of uropathogenic Escherichia coli

Ranjbar¹,

Dehkordi²,

Mashhouri³

et al. 2020

Preprint

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Background: The aim of this study was genotyping of Uropathogenic Escherichia coli (UPEC) based on Variable Number of Tandem Repeats (VNTRs) sequences. Methods: E. coli strains isolated from urine samples were included in this study. Seven VNTR loci were subjected to Multilocus variable-number tandem repeat analysis (MLVA) based on PCR amplification. Then data was analyzed via online mlvaplus software and the information was displayed in the form of MST analysis. Results: A total of 100 E. coli strains were isolated and subjected to the study. MLVA was able to differentiate 56 different genotypes. Also, the technique could classify E. coli isolates in 5 clonal complexes. Based on UPGMA dendrograms, E. coli isolates were classified into 4 clusters (clusters A to D). The strains associated with Complex No. 1 appeared to be dominant pathogens of UPEC in Tehran's patients. The present study provides valuable insights into the genetic relationships of E. coli isolates recovered from clinical cases in a major hospital in Iran. Conclusions: The analysis of MLVA profiles using the MST algorithm showed the usefulness of the MLVA method in the classification of uropathogenic E. coli collected in different periods. We evaluated MLVA in a laboratory equipped with simple molecular equipment. Based on these results, it has been assumed that the E. coli strains were derived from a limited number of clones that have undergo a small genetic change during this period.

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Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic Escherichia coli Recovered from Hospital Food

Cited by 15 publications

References 46 publications

Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

<p>Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of <em>vacA</em>, <em>cagA</em>, <em>iceA</em>, <em>oipA</em>, <em>cagE</em>, and <em>babA2</em> Alleles of <em>Helicobacter pylori</em> Bacteria Isolated from Raw Meat</p>

Multilocus Variable Number of Tandem Repeat Analysis for molecular typing of uropathogenic Escherichia coli

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