Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

Mousavi, Reza; Rahimi, Ebrahim; Shakerian, Amir

doi:10.21608/ejvs.2020.20516.1141

Cited by 5 publications

(3 citation statements)

References 30 publications

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“…E. coli strains are utilized as sentinel organisms for the surveillance of AMR with gram-negative resistance characteristics and as indicators of fecal contamination of food items since they are common in humans, animals, the environment, and foods (WHO, 2017). Regarding with the obtained results of E. coli sensitivity test (Tables 6 to 8), it came in the same line with those recorded by Kim et al (2020), who recorded a significant resistance of E. coli to ampicillin (69.1%) and tetracycline (64.0%); Mousavi et al (2020) where their E. coli isolates exhibited resistance against ampicillin (95.45%), tetracycline (88.63%), gentamycin (84.09%) and Sulfa/trimethoprim (38.63%) antibiotics, while were sensitive to chloramphenicol (72.73%). Mensah et al (2022) recorded that all of their examined E. coli isolates were sensitive to gentamicin and cefotaxime.…”

Section: Discussionsupporting

confidence: 88%

Tracking the antibiotic resistance phenomenon in poultry meats under species and seasonal variations

Farouk,

Salem,

Nassif

2023

Benha Veterinary Medical Journal

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Antimicrobial-resistant bacteria in poultry meat pose a threat to public health. A total of ninety E. coli and S. aureus strains were isolated from 270 raw poultries (chicken, quail, and duck) drumstick samples purchased from local butchers in the Qalyubia governorate along winter, spring, and summer seasons of 2022 (30 isolates of each season). Isolates of each species per each season were examined for their antibiotic sensitivity using eight antibiotics represented by amoxicillin/clavulanic acid, ampicillin, ceftriaxone, ciprofloxacin, gentamicin, tetracycline, trimethoprim/sulfamethoxazole, and chloramphenicol by disc diffusion technique. Results revealed that the examined E. coli and S. aureus isolates had a variable sensitivity profile, where S. aureus showed higher resistance affinity than E. coli. Although the tested antibiotics showed variable sensitivity ratios, tetracycline showed the lowest sensitivity ratio either on S. aureus or E. coli. Isolates collected from duck samples showed higher resistance activity than those of chicken and quail, respectively. Moreover, the examined isolates detected in the summer season were more resistant to the tested antibiotics than those examined in spring and winter, respectively. In conclusion, fast-growing drug resistance of foodborne bacteria, especially, S. aureus and E. coli, of poultry meat origin appeared to be a critical health problem, especially during hot and humid weather of the summer season. So, close surveillance is required throughout the poultry production chain to prevent the spread of antimicrobial-resistant bacterial strains.

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Section: Discussionsupporting

confidence: 88%

Tracking the antibiotic resistance phenomenon in poultry meats under species and seasonal variations

Farouk,

Salem,

Nassif

2023

Benha Veterinary Medical Journal

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“…This could explain the severe pathogenicity of these stains and underline the role of quails in transmission of antibiotic resistance bacteria to humans. Previous studies also reported the presence of virulent and antibiotic resistant E. coli strains in poultry meat (Ammar et al, 2015;Himi et al, 2015;Eid et al, 2016;Prihtiyantoro et al, 2019;Mousavi et al, 2020).…”

Section: Disscusionmentioning

confidence: 84%

Prevalence of Virulence Factors and Antibiotic Resistance Genes in Shiga Toxin-Producing Escherichia Coli Isolated From Quails

2020

Assiut Veterinary Medical Journal

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This study aimed to determine the prevalence, pathological investigation and profiles of virulence and antibiotic resistance genes in Shiga toxin-producing Escherichia coli (E. coli) isolated from quails. A total of 450 samples were collected from 150 quails (50apparently healthy, 50 diseased and 50 recently dead) for bacterial isolation. The isolated E.coli was tested for antimicrobial susceptibility and was screened for the presence of virulence genes (stx1, stx2, eaeA, and hly) and antibiotic resistance genes (tetAandsul1) using polymerase chain reaction (PCR). Seven-day old quails were challenged with E. coli to determine the pathogenicity of the isolated strains. A total number of 93 (20.67%) E. coli isolates were recovered. The most prevalent serogroups O125, O20, and O78. E. coli were highly sensitive to neomycin, norfloxacin, and, colistin, highly resistant to sulphamethoxazole, tetracycline, and amoxicillin. Stx2 and eaeA genes were detected in (3/8) and (2/8) of isolates, respectively, while all isolates were negative for stx1 and hly genes. Moreover, tetA and sul1 genes were detected in 100% of examined isolates. The pathogenicity test revealed that E .coli (O125 STEC) was a highly pathogenic and induced mortality rate of 40%. Histopathological changes supported the post mortem lesions. In conclusion, these data can be considered alarming since quail may be a carrier of STEC that is highly pathogenic for humans. Also, these E. coli showed marked resistance to several antibiotics of clinical interest. So proper hygiene regime and biosecurity measures are necessary to minimize the risk of spread of infection to the human population.

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“…The main reasons for the interest in poultry meat are its relatively low production costs and the absence of cultural and religious restrictions on its consumption [10]. Contamination of poultry meat can occur at various stages of its slaughter and processing [11,12]. Studies have shown that H. pylori can survive in the gastrointestinal tract of chickens, and these bacteria can be present in their feces [13].…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori Strains Isolated from Raw Poultry Meat in the Shahrekord Region, Iran: Frequency and Molecular Characteristics

Asadi

Rahimi

Shakerian

2023

Genes

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Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (chicken, turkey, quail, goose, and ostrich) is consumed as a regular protein source by many people across the world; therefore, sanitary ways of delivering poultry for food are important for global health. As a result, the distribution of the virulence genes cagA, vacA, babA2, oipA, and iceA in H. pylori isolates in poultry meat, as well as their antibacterial resistance, was investigated. A Wilkins Chalgren anaerobic bacterial medium was used to cultivate 320 samples of raw poultry meat. Disk diffusion and multiplex-PCR were used to investigate both antimicrobial resistance and genotyping patterns. H. pylori was found in 20 of 320 (6.25 %) raw chicken meat samples. The highest incidence of H. pylori was found in chicken raw meat (15%), whereas no isolate was recovered from goose or quail raw meat (0.00%). Resistance to ampicillin (85%), tetracycline (85%), and amoxicillin (75%) were the most commonly detected in the tested H. pylori isolates. The percentage of H. pylori isolates with a multiple antibiotic resistance (MAR) index value of more than 0.2 was 17/20 (85%). The most prevalent genotypes detected were VacA (75%), m1a (75%), s2 (70%) and m2 (65%), and cagA (60%). The most typically detected genotype patterns were s1am1a (45 %), s2m1a (45 %), and s2 m2 (30 %). babA2, oipA+, and oipA- genotypes were found in 40%, 30%, and 30% of the population, respectively. In summary, fresh poultry meat was polluted by H. pylori, with the babA2, vacA, and cagA genotypes being more prevalent. The simultaneous occurrence of vacA, cagA, iceA, oipA, and babA2 genotypes in antibiotic-resistant H. pylori bacteria raises a serious public health concern regarding the consumption of raw poultry. Future research should evaluate antimicrobial resistance among H. pylori isolates in Iran.

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Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

Cited by 5 publications

References 30 publications

Tracking the antibiotic resistance phenomenon in poultry meats under species and seasonal variations

Tracking the antibiotic resistance phenomenon in poultry meats under species and seasonal variations

Prevalence of Virulence Factors and Antibiotic Resistance Genes in Shiga Toxin-Producing Escherichia Coli Isolated From Quails

Helicobacter pylori Strains Isolated from Raw Poultry Meat in the Shahrekord Region, Iran: Frequency and Molecular Characteristics

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