Prevalence of Anti-Toxoplasma Gondii Antibodies in Aborted Ewes in Kuwait

Toxoplasmosis is a worldwide zoonosis caused by the parasitic protozoan Toxoplasma gondii. It can infect all warm‐blooded vertebrate species and causes abortions and birth defects in pregnant women and pregnant ewes. The objective of this study was to estimate the prevalence of infection with T. gondii in sheep meat in the region of Sidi Bouzid (central Tunisia) and Beja (northern Tunisia), the realization of a descriptive study of risk factors and the phylogenetic analyses of T. gondii. Neck muscle samples were obtained from 174 ewes and ewe lamb slaughtered in Sidi Bouzid and 150 lambs slaughtered in Beja. DNA was extracted from the samples using the Wizard® genomic DNA purification kit. A nested PCR using two pairs of primers (NN 1 and NN2, Tg‐NP1 and Tg‐NP2) were used to detect infection with T. gondii, which was then confirmed by sequencing. Eight T. gondii amplicons were sequenced (accession number KT896498) and deposited in GenBank. The T. gondii amplicons showed 97–100% identities with GenBank sequences. A phylogenetic tree was then constructed. The nested PCR detected T. gondii DNA in 31% of animals tested in Sidi Bouzid and 32% of lambs tested in Beja. No significant difference in the prevalence of T. gondii infection was established between the two tested regions. In both regions, no significant variation of the infection depending on age, breed and locality was found.

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“…; Leblebicier & Yildiz ). In Asia, prevalence varied between 17.8% in Kuwait (Alazemi ) and 69.9% in Bangladesh (Rahman et al . ).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and phylogenetic analyses ofToxoplasma gondiifrom naturally infected sheep in Northern and Central Tunisia

et al. 2016

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“…It is worth mentioning that some of the aforementioned countries are examples of major exporters of sheep and livestock to countries in the Arabian Peninsula, which might explain the relatively high prevalence of T. gondii infection among this animal population in this region. Furthermore, some studies from Saudi Arabia and Kuwait examined ewes that went through abortion and linked that to their infection by T. gondii , as bradyzoites were commonly detected in aborted foetuses (Alazemi, 2014; Hussein et al., 2011). This outlines how toxoplasmosis can be an important cause of reproductive wastage in small ruminants, which may present a serious economic challenge to exporting countries (Hussein et al., 2011).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Toxoplasma gondii infection in animals of the Arabian Peninsula between 2000–2020: A systematic review and meta‐analysis

Abdelgadier

Assaad

Elhussein

et al. 2022

Veterinary Medicine & Sci

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Background Toxoplasma gondii ( T. gondii ) is a zoonotic parasite that can be transmitted from animals to humans, with felids acting as its definitive host. Thus, understanding the epidemiology of this parasite in animal populations is vital to controlling its transmission to humans as well as to other animal groups. Objectives This systematic review and meta‐analysis aims to summarise and analyse reports of T. gondii infection in animal species residing in the Arabian Peninsula. Methods : It was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA), with relevant studies being retrieved from MEDLINE/PubMed, Scopus, Cochrane Library, Google Scholar and ScienceDirect. All articles published in Arabic or English languages between January 2000 and December 2020 were screened for eligibility. Random effects model was used to calculate the pooled prevalence of T. gondii infection in different animal populations which were found to harbour this infection. The critical appraisal tool for prevalence studies designed by the Joanna Briggs Institute (JBI) was used to assess the risk of bias in all included studies. Results A total of 15 studies were retrieved, reporting prevalence estimates from 4 countries in this region and in 13 animal species. Quantitative meta‐analysis estimated a pooled prevalence of 43% in felids [95% confidence interval (CI) = 23–64%, I 2 index = 100%], 48% in sheep (95% CI = 27–70%, I 2 = 99%) and 21% in camels (95% CI = 7–35%, I 2 = 99%). Evidence of possible publication bias was found in both felids and sheep. Conclusions This meta‐analysis estimates a high prevalence of T. gondii infection in animal species which are of high economic and cultural importance to countries of this region. Hence, these findings provide valuable insight to public health authorities as well as economic and animal resources advisors in countries of the Arabian Peninsula.

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Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt

et al. 2018

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Toxoplasmosis is an infectious zoonotic disease caused by protozoan Toxoplasma gondii. Detection of T. gondii infection with touchy and particular strategies is a key advance to control and prevent toxoplasmosis. Genotyping can explain the virulence, epidemiology and setting up new methodologies for diagnosis and control in human and animals. The point of this study was to assess the seroprevalence of T. gondii in sheep and goat in Egypt and to comprehend the genetic variety of T. gondii isolates circling in Egypt. Blood samples were gathered from 113 ewes and 95 she-goats from three Egyptian governorates (Cairo, Giza and Al-Sharkia). Also blood and tissue samples were gathered from 193 sheep and 51 goats from Cairo and Giza abattoirs. All samples were assayed serologically utilizing ELISA and OnSite Toxo IgG/IgM Rapid test cassettes (OTRT) tests and the tissue samples of the seropositive animals were digested and microscopically examined then bio-assayed in mice as viability test. All the T. gondii isolates undergo molecular identification using PCR and genotyped utilizing nPCR/RFLP analysis of SAG2 gene. The total seropositivity of live sheep and goat was 47.15 and 39.2% utilizing ELISA and OTRT respectively. Concerning abattoirs, seropositivity, positive microscopic examination, mice viability from sheep samples were 47.1%, 37.3% and 44.1% respectively while that of goats were 45.5%, 33.3% and 48.6% respectively. Eighteen T. gondii isolates were affirmed utilizing PCR. Genotyping confirmed 10 isolates (55.5%) as type II, 6 (33.3%) as type III and 2 (11.1%) as atypical genotypes. Type II and III are the genotypes mostly circling among small ruminants in Egypt and this is most significance for the public health in Egypt.

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Prevalence of Anti-Toxoplasma Gondii Antibodies in Aborted Ewes in Kuwait

Cited by 8 publications

References 22 publications

Molecular detection and phylogenetic analyses ofToxoplasma gondiifrom naturally infected sheep in Northern and Central Tunisia

Molecular detection and phylogenetic analyses ofToxoplasma gondiifrom naturally infected sheep in Northern and Central Tunisia

Prevalence of Toxoplasma gondii infection in animals of the Arabian Peninsula between 2000–2020: A systematic review and meta‐analysis

Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt

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