Molecular detection and phylogenetic analyses of<i>Toxoplasma gondii</i>from naturally infected sheep in Northern and Central Tunisia

Rouatbi, Mariem; Amdouni, Yosra; Amairia, Safa; Rjeibi, Mohamed Ridha; Sammoudi, Said; Rekik, Mourad; Gharbi, Mohamed

doi:10.1002/vms3.53

Cited by 11 publications

(1 citation statement)

References 42 publications

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“…For the second round PCR, 2 μl of the first-round PCR product, diluted to 1:5 with molecular grade water (Qiagen, Valencia, CA) was used as the template for the second-round PCR in a total volume of 20 μl under the same PCR conditions as in the first round, using the internal primers NNP1 forward primer (5′-GTGATAGTATCGAAAGGTAT-3′) and NP2 reverse primer (5′ACTCTCTCTCAAATGTTCCT-3′) as previously described. [ 32 ]. Positive controls (2 μL T. gondii DNA) and negative controls (2 μL reagent grade H 2 O) were included in each PCR run.…”

Section: Methodsmentioning

confidence: 99%

Toxoplasma gondii seroprevalence among pregnant women attending antenatal clinic in Northern Tanzania

et al. 2018

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BackgroundAcute Toxoplasma gondii infection during pregnancy represents a risk for congenital disease, especially among women without previous exposure to infection. There is, however, a paucity of information about the epidemiology of T. gondii infection in pregnant women in Tanzania. This study aimed to determine the seroprevalence of T. gondii infection and associated demographic, clinical, and behavioral risk factors in pregnant women attending ante-natal clinic (ANC) at Kilimanjaro Christian Medical Center (KCMC), a referral medical center in Northern Tanzania.MethodsA hospital-based cross-sectional study was carried out from 1 February to 30 April 2017. Data on maternal demographic characteristics, obstetric history, knowledge, and practices related to T. gondii infection were collected from 254 pregnant women attending antenatal care at KCMC. A sample of 4 mL of blood was collected from each participant and sera prepared from each sample. Serum samples were tested for the presence of specific T. gondii IgG and IgM antibodies by indirect Enzyme-Linked Immunosorbent Assay (ELISA). DNA was extracted from whole blood for polymerase chain reaction (PCR) testing, targeting the DNA sequence coding for the Internal Transcribed Spacer 1 (ITS1).ResultsThe overall T. gondii seroprevalence, including both IgM- and IgG-positive individuals, was 44.5%. Of the 254 tested women, 102 and 23 were seropositive for T. gondii-specific IgG and IgM antibodies respectively and 113 individuals had antibodies of either or both classes. All IgM-positive samples were also tested by PCR, and all were negative. The majority (90%) of the women surveyed had never heard about toxoplasmosis. Consumption of raw vegetables [aOR = 0. 344; 95% CI 0.151–0.784; p = 0.011] and having regular contact with soil [aOR = 0.482; 95% CI 0.268–0.8681; p = 0.015] were both associated with T. gondii antibody status. Inverse relationships with probability of T. gondii exposure were observed, such that these practices were associated with reduced probability of antibody detection.ConclusionBased on serology results, we report widespread exposure to T. gondii infection among pregnant women attending ANC in KCMC. The complex interaction of risk factors for T. gondii infection needs to be studied in larger longitudinal studies.

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Section: Methodsmentioning

confidence: 99%

Toxoplasma gondii seroprevalence among pregnant women attending antenatal clinic in Northern Tanzania

et al. 2018

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A Meta-Analysis of the Prevalence of Toxoplasmosis in Livestock and Poultry Worldwide

et al. 2022

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Toxoplasma gondii causes toxoplasmosis with a global prevalence in the world. A large proportion of human illness is most frequently associated with consuming raw and undercooked meat or other animal products containing infective parasitic stages of T. gondii . This systematic review and meta-analysis study evaluated the prevalence of toxoplasmosis in cattle, sheep, camels, goats, and poultry worldwide. The search was performed in databases including PubMed, WoS, Scopus, Science Direct, Google Scholar, and ISC from 2000 to 2019 in Persian and English. The main inclusion criteria were the prevalence of toxoplasmosis among livestock and poultry and the prevalence indices by sample size. During these 20 years, the overall prevalence of toxoplasmosis in livestock and poultry was 28.3% (95% confidence interval (CI) 25–31.9%) using the random-effects meta-analysis model. The highest prevalence of T. gondii in livestock and poultry animals was found in Asia in 2014 with 89.8% (95% CI 78.5–95.5%). The lowest prevalence was found in Asia in 2013 with 1.26% (95% CI 0.4–3.8%). A quarter of livestock and poultry were infected with T. gondii . Since livestock products are globally important sources of people's diet, our findings are useful for policymakers to control T. gondii infection in livestock. Supplementary Information The online version contains supplementary material available at 10.1007/s10393-022-01575-x.

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Serological survey for anti-Toxoplasma gondii antibodies in sheep of northeastern Algeria

Benlakehal¹,

Miroud²,

Djeghim³

et al. 2019

Trop Anim Health Prod

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Molecular detection and phylogenetic analyses ofToxoplasma gondiifrom naturally infected sheep in Northern and Central Tunisia

Cited by 11 publications

References 42 publications

Toxoplasma gondii seroprevalence among pregnant women attending antenatal clinic in Northern Tanzania

Toxoplasma gondii seroprevalence among pregnant women attending antenatal clinic in Northern Tanzania

A Meta-Analysis of the Prevalence of Toxoplasmosis in Livestock and Poultry Worldwide

Serological survey for anti-Toxoplasma gondii antibodies in sheep of northeastern Algeria

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