Prevalence of Ambler class A and D β-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea

Lee, Seungok; Park, Yeon-Joon; Kim, Myungshin; Lee, Hae Kyung; Han, Kyungja; Kang, Chang Suk; Kang, Moon Won

doi:10.1093/jac/dki160

Cited by 110 publications

(73 citation statements)

References 27 publications

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“…DNA extraction was performed by the simple boiling method of bacterial colonies from all isolates that were identified as P. aeruginosa by phenotypic criteria (13).…”

Section: Polymerase Chain Reaction Amplification Of the Mexa And Mexbmentioning

confidence: 99%

Efflux MexAB-Mediated Resistance in Multidrug and Pan-Drug Resistant Strains of Pseudomonas aeruginosa Isolated From Patients With Burn and Wound Infections

Khosravi

Mohammadian

2016

Jundishapur J Nat Pharm Prod

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Background: The emergence of highly drug resistant Pseudomonas aeruginosa in burn wounds is becoming a challenging problem for infection control programs. Today, it has been shown that antibiotic resistance in P. aeruginosa is the result of synergism between membrane permeability and multidrug resistance (MDR) efflux pumps. Objectives: The aim of this study was to investigate MDR and pan-drug resistance (PDR) in P. aeruginosa and detection of the presence of efflux pump MexAB genes by the polymerase chain reaction (PCR) technique. Materials and Methods: One-hundred and fifty P. aeruginosa were isolated from burn and wound infections. The isolates were confirmed using conventional culture and biochemical tests. Antibiotic resistance was evaluated using agar disk diffusion and broth micro dilution tests. For detection of efflux pump MexAB genes, the PCR technique with subsequent sequencing was used. Results: In total, 99 strains (66%) were MDR and one strain (0.667%) was PDR as detected by traditional susceptibility tests. The MDR isolates belonged to 53 burn (70.66%) and 46 wound (61.33%) infections. The PDR was only seen in one isolate from a burn strain (1.33%). The PCR technique revealed that all the 99 MDR strains and one PDR strain, contained MexA and MexB genes, representing an exhibitive intrinsic existence of these genes in MDR and PDR of P. aeruginosa. Conclusions: This study represented an increasing rate of MDR P. aeruginosa in burn and wound samples. Efflux MexAB genes were detected in all MDR and PDR strains. The P. aeruginosa strain isolated from burn cases showed higher drug resistance and PDR resistance was only noted in a burn sample.

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“…DNA extraction was performed by the simple boiling method of bacterial colonies from all isolates that were identified as P. aeruginosa by phenotypic criteria (13).…”

Section: Polymerase Chain Reaction Amplification Of the Mexa And Mexbmentioning

confidence: 99%

Efflux MexAB-Mediated Resistance in Multidrug and Pan-Drug Resistant Strains of Pseudomonas aeruginosa Isolated From Patients With Burn and Wound Infections

Khosravi

Mohammadian

2016

Jundishapur J Nat Pharm Prod

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“…The bla SHV and bla TEM were amplified as described previously [5]. Plasmid DNA was extracted by miniprep kit (Metabion, Martinsried, Germany) and targeted for the bla CTX-M gene using the primers described by Lee et al [12] under the following conditions: 94°C for 3 minutes, and 35 cycles of 94°C for 30 seconds, 63°C for 1 minute, 72°C for 1 minute and a final extension in 72°C for 10 minutes. K. pneumoniae ATCC 700603, E. coli ATCC 35218 and K. pneumoniae 7881 were used as positive controls for bla SHV , bla TEM and bla CTX-M , respectively.…”

Section: Pcr Amplification Of Beta-lactamase Genesmentioning

confidence: 99%

Genetic characterization of ESBL producing strains of Klebsiella pneumoniae from Tehran hospitals

Feizabadi

Mahamadi-Yeganeh

Mirsalehian

et al. 2010

J Infect Dev Ctries

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Introduction: This study was conducted to determine the genetic characterization of extended-spectrum beta-lactamase (ESBL) producing strains of Klebsiella pneumoniae isolated from Iranian patients in hospitals in Tehran. Methodology: Antibiotic susceptibility of 104 isolates was determined using the disk diffusion test. The Minimum Inhibitory Concentrations (MICs) of imipenem and meropenem were determined for isolates showing reduced susceptibility to carbapenems. The phenotypic confirmatory test (PCT) was used to screen the isolates for ESBL production. PCR was used to detect bla SHV , bla TEM and bla CTX-M and the amplicons from selected clones were sequenced. Isolates producing ESBLs were analyzed by pulsed-field gel electrophoresis (PFGE). Results: One isolate showed resistance to imipenem (MIC = 16 µg/ml). Resistance to amikacin and ciprofloxacin was 44.2% and 25.0%, respectively. ESBL production was detected in 72.1% (n = 75) of isolates. The prevalence of bla SHV , bla TEM and bla CTX-M genes among the isolates was 55.7% (n = 58), 30.7% (n = 32) and 45.2% (n = 47), respectively. The sequencing revealed the amplicons corresponding to bla

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“…Farshadzadeh et al, in a study carried out at the Taleghani Burn Hospital in Ahvaz (Iran), reported that 54.2% and 68.7% of ESBL-producing isolates of P. aeruginosa harbored bla PER-1 and bla OXA-10 , respectively 29 . Among the studies reported from Asian countries, Lee and colleagues from South Korea showed that 6.3%, 13.1%, 4.3%, 2.0%, 2.3%, and 0.4% of P. aeruginosa isolates carried the bla PSE-1 , bla , bla OXA-4 , bla OXA-30 , bla OXA-2 , and bla OXA-17 genes, respectively 36 . In another study performed in Saudi Arabia, the presence of genes encoding VEB-1, OXA-10, and GES was confirmed among extended-spectrum cephalosporin-non-susceptible P. aeruginosa clinical isolates from burn patients 10 .…”

mentioning

confidence: 99%

Distribution of the bla OXA , bla VEB-1 , and bla GES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran

Amirkamali

Naserpour-Farivar

Azarhoosh

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:Pseudomonas aeruginosa is one of the most common nosocomial pathogens. The emergence of extended spectrum β-lactamases (ESBLs) has been increasingly reported as a major clinical concern worldwide. The main aim of the present study was to determine the distribution of bla OXA , bla PER-1, bla VEB-1, and bla GES-1 genes among ESBL-producing P. aeruginosa isolated from two distinct provinces in Iran. Methods: In this study, a total of 75 (27.5%) ESBL-producing isolates were identified from 273 P. aeruginosa isolates collected from patients in Qazvin and Tehran. Phenotypic detection of ESBLs and antimicrobial susceptibility testing were performed according to the Clinical and Laboratory Standards Institute guidelines. PCR and sequencing were employed to detect bla OXA-1 , bla OXA , bla GES-1, bla PER-1 , and bla VEB-1 genes. Isolate genetic relationships were evaluated by repetitive extragenic palindromic sequence-based PCR (REP-PCR). Results: In total, 59 (78.7%) of the ESBLproducing isolates showed multidrug resistance. The highest rates of susceptibility were observed against colistin (75 isolates, 100%) and polymyxin B (75, 100%) followed by amikacin (44, 58.7%), and piperacillin-tazobactam (40, 53.3%). The bla OXA-1 (37.3%) gene was the most common of the genes investigated, followed by bla OXA-4 (32%), bla GES-1 (16%), and bla VEB-1 (13.3%). REP-PCR identified three different genotypes: types A (89.3%), B (6.7%), and C (4%). Conclusions: We found a significant presence of bla OXA-1 , bla OXA-4 , bla GES-1, and bla VEB-1 genes among P. aeruginosa isolates, highlighting the need for suitable infection control strategies to effectively treat patients and prevent the further distribution of these resistant organisms.

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Prevalence of Ambler class A and D β-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea

Cited by 110 publications

References 27 publications

Efflux MexAB-Mediated Resistance in Multidrug and Pan-Drug Resistant Strains of Pseudomonas aeruginosa Isolated From Patients With Burn and Wound Infections

Efflux MexAB-Mediated Resistance in Multidrug and Pan-Drug Resistant Strains of Pseudomonas aeruginosa Isolated From Patients With Burn and Wound Infections

Genetic characterization of ESBL producing strains of Klebsiella pneumoniae from Tehran hospitals

Distribution of the bla OXA , bla VEB-1 , and bla GES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran

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