Prevalence, Morphological and Molecular Phylogenetic Analyses of the Rabbit Pinworm, Passalurus ambiguus Rudolphi 1819, in the Domestic Rabbits Oryctolagus cuniculus

Abdel‐Gaber, Rewaida; Ataya, Farid S.; Fouad, Dalia; Daoud, Mohamed S.; Al-Zuhairy, Shatha S.

doi:10.2478/s11686-019-00047-7

Cited by 17 publications

(12 citation statements)

References 48 publications

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“…Одним із факторів, що забезпечують ефективне введення галузі та збільшення виробництва продукції кролівництва, є ветеринарне благополуччя господарств щодо паразитарних хвороб кролів, зокремапасалурозу (Robinson & Chalmers, 2010;Hernandez et al, 2018;Abdel-Gaber et al, 2019;Mykhailiutenko et al, 2019). Так, на території Північно-Східної Шотландії 14,2 % досліджених домашніх кролів були уражені збудником пасалурозу (Boag, 1985).…”

Section: вступunclassified

The effectiveness of the proposed method of culturing eggs of nematodes Passalurus ambiguus, parasitic in rabbits

2021

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Rabbit breeding is one of the promising branches of meat cattle breeding, and also plays an important role in supplying light industry with fur raw materials. One of the factors that ensure the efficient management of the industry and increase the production of rabbit products is the veterinary welfare of farms against parasitic diseases of rabbits, in particular – pasalurosis. It is necessary to prevent the development of the parasite at all stages in order to successfully control and prevent pasalurosis. The aim of the work was to improve and determine the effectiveness of the method of cultivation of Passalurus ambiguus eggs, which can be used to study the embryogenesis of the pathogen pasalurosis in rabbits, obtain a laboratory model of invasion, determine the disinvasive properties of disinvasive and disinfectants and agents. The proposed method of culturing P. ambiguus nematode eggs allows to take into account the sensitivity of eggs to moisture, allows to obtain a high yield of invasive eggs in the experimental culture, as well as to conduct microscopic examinations and microphotography without removing eggs from the substrate in which cultivation takes place. Eggs of P. ambiguus were obtained from the gonads of females of the pathogen pasalurosis, which were removed by helminthic dissection of the colon of rabbits. The proposed method is based on the use of nutrient environment for the cultivation of P. ambiguus eggs. Studies have shown that the improved method was more effective than the well-known method, where as a substrate for the cultivation of Pasaluris eggs used saline. With the use of the improved method, the invasive stage reaches more than 71 % of Pasaluris eggs, which is 38.75% more (P < 0.001) than with the use of the well-known method of cultivation. The obtained results of the conducted research activities lead to further, deeper study of questions concerning biological features of embryonic development of the causative agent of passalurosis, and also disinvasive efficiency of modern disinfectants concerning P. ambiguus eggs.

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Section: вступunclassified

The effectiveness of the proposed method of culturing eggs of nematodes Passalurus ambiguus, parasitic in rabbits

2021

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“…, Passalurus ambiguus , Cryptosporidium spp. and Giardia duodenalis ) have been identified as pathogens which cause diarrhoea in rabbits [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. In addition to the above pathogens, Cyniclomyces guttulatus , a commensal yeast in the rabbit gastrointestinal tract, is also commonly seen in diarrhoea cases.…”

Section: Introductionmentioning

confidence: 99%

An Investigation of the Relationship between Cyniclomyces guttulatus and Rabbit Diarrhoea

et al. 2021

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Cyniclomyces guttulatus is usually recognised as an inhabitant of the gastrointestinal (GI) tract in rabbits. However, large numbers of C. guttulatus are often detected in the faeces of diarrhoeic rabbits. The relationship of C. guttulatus with rabbit diarrhoea needs to be clearly identified. In this study, a C. guttulatus Zhejiang strain was isolated from a New Zealand White rabbit with severe diarrhoea and then inoculated into SPF New Zealand white rabbits alone or co-inoculated with Eimeriaintestinalis, another kind of pathogen in rabbits. Our results showed that the optimal culture medium pH and temperature for this yeast were pH 4.5 and 40–42 °C, respectively. The sequence lengths of the 18S and 26S ribosomal DNA fragments were 1559 bp and 632 bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from dogs and 100% homology with the 26S ribosomal sequence of DPA-CGR1 and CGDPA-GP1 isolates from rabbits and guinea pigs, respectively. In animal experiments, the C. guttulatus Zhejiang strain was not pathogenic to healthy rabbits, even when 1 × 108 vegetative cells were used per rabbit. Surprisingly, rabbits inoculated with yeast showed a slightly better body weight gain and higher food intake. However, SPF rabbits co-inoculated with C. guttulatus and E. intestinalis developed more severe coccidiosis than rabbits inoculated with C. guttulatus or E. intestinalis alone. In addition, we surveyed the prevalence of C. guttulatus in rabbits and found that the positive rate was 83% in Zhejiang Province. In summary, the results indicated that C. guttulatus alone is not pathogenic to healthy rabbits, although might be an opportunistic pathogen when the digestive tract is damaged by other pathogens, such as coccidia.

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“…Eimeria spp., Passalurus ambiguus, Cryptosporidium spp. and Giardia duodenalis) have been identified as pathogens causing diarrhea in rabbits [4][5][6][7][8][9][10][11][12][13][14][15] . In addition to the above pathogens, Cyniclomyces guttulatus, a commensal yeast in rabbit gastrointestinal tract is also commonly seen in diarrhea cases.…”

Section: Introductionmentioning

confidence: 99%

Cyniclomyces guttulatusis an opportunistic pathogen in rabbits with coccidiosis

Shi¹,

Sun²,

Fu³

et al. 2019

Preprint

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Cyniclomyces guttulatus is a common inhabitant of the gastrointestinal tract in rabbits, and large numbers are often present in feces of diarrheic rabbits. However, its relation with rabbit diarrhea has not been clearly identified. We isolated a C. guttulatus Zhejiang strain from a rabbit with severe diarrhea and optimized the culture conditions in YPG medium. The sequenced 18S and 26S ribosomal DNA fragments were 1559bp and 632bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from the dog and 100% homology with the 26S ribosomal sequence of the DPA-CGR1 and CGDPA-GP1 isolates from the rabbit and guinea pig. Our isolate was not pathogenic to healthy SPF rabbits. Instead, rabbits inoculated with the yeast had a slightly better body weight gain and higher food intake. Rabbits co-inoculated with C. guttulatus and the coccidian, E. intestinalis developed more severe coccidiosis as shown by clinical signs, and decreased body weight gain, diarrhea and death, associated with significantly higher fecal output of C. guttulatus vegetative cells but lower coccidian oocysts output than the rabbits inoculated with C. guttulatus or E. intestinalis alone. We also surveyed the prevalence of C. guttulatus in rabbits and found a positive rate of 83% in Zhejiang province. Our results indicate that C. guttulatus alone is not pathogenic to healthy rabbits, but could become an opportunistic pathogen when the digestive tract is damaged by other pathogens such as coccidia.Author summary: Cyniclomyces guttulatus, a commensal yeast in rabbit gastrointestinal tract, is very commonly seen in diarrhea cases. However, it is unclear whether it causes or is a co-cause of diarrhea with other pathogens. Here, a C. guttulatus Zhejiang strain was firstly isolated from a rabbit with severe diarrhea and the culture conditions in YPG medium were optimized. Then, it was identified in morphology and molecular. It was agreed with the previous description in morphology and showed a closer phylogenetic relationship with other strains originated from herbivores than those from the carnivore. Finally, the C. guttulatus Zhejiang strain was inoculated to SPF rabbits singly or co-inoculated with Eimeria intestinalis. All of the results in animal assays show C. guttulatus alone is not pathogenic but seems a probiotic microorganism in rabbits. However, it could become an opportunistic pathogen when the digestive tract is damaged by other pathogens such as coccidia.

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Prevalence, Morphological and Molecular Phylogenetic Analyses of the Rabbit Pinworm, Passalurus ambiguus Rudolphi 1819, in the Domestic Rabbits Oryctolagus cuniculus

Cited by 17 publications

References 48 publications

The effectiveness of the proposed method of culturing eggs of nematodes Passalurus ambiguus, parasitic in rabbits

The effectiveness of the proposed method of culturing eggs of nematodes Passalurus ambiguus, parasitic in rabbits

An Investigation of the Relationship between Cyniclomyces guttulatus and Rabbit Diarrhoea

Cyniclomyces guttulatusis an opportunistic pathogen in rabbits with coccidiosis

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