The effectiveness of the proposed method of culturing eggs of nematodes Passalurus ambiguus, parasitic in rabbits

Євстаф’єва, В. О.; Khorolskyi, A.; Мельничук, В. В.

doi:10.32718/nvlvet10105

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…These are flotation methods and their modifications, which are based on the principle of the difference in the specific gravity of eggs and hypertonic saline solutions (Knopp et al, 2009;Dakhno & Dakhno, 2010;Lobos-Ovalle et al, 2021). At the same time, a significant number of researchers propose improved, more modern, and effective methods of coproovoscopy for individual parasitosis (Yevstafieva, 2007a;Manoilo & Yevstafieva, 2016;Starodub, 2019). Therefore, the development and testing of an improved method of coproovoscopy for trichurosis in sheep is urgent.…”

Section: Discussionmentioning

confidence: 99%

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Effectiveness of the improved method of lifelong diagnostics trichurosis of sheep

Petrenko

2023

Ukr. Jour. of Vet. and Agr. Sci.

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Today, the most accurate way to diagnose animal helminthiasis is laboratory research. Among them, lifelong coproovoscopy methods play a leading role in the study of the spread of gastrointestinal helminthiasis, particularly trichurosis, and in establishing the effectiveness of anthelmintics. Therefore, a promising research direction includes improving and testing modern methods of coproovoscopy for trichurosis in sheep. The work aimed to determine the diagnostic efficiency of the improved flotation method of coproovoscopy for trichurosis in sheep. In laboratory conditions, the effectiveness of well-known flotation methods and the proposed method of coproovoscopy in diagnosing sheep trichurosis were determined. The leading indicators of the effectiveness of laboratory methods were the indicator of the intensity of trichurous invasion, the coagulation ability of the flotation solution, and the crystallization time of a drop of flotation liquid on a glass slide. It was established that all methods of coproovoscopy used in the experiment have flotation properties relative to Trichuris eggs. However, the improved method of coproovoscopy showed a higher flotation ability concerning Trichuris ovis nematode eggs, where the proposed flotation liquid has pronounced coagulation properties concerning undigested feed residues, and also has a slow crystallization time of droplets on a slide, which is more than 50 min. The improved method uses a combined flotation liquid, whose main components were Ca(NO3)2, C12H22O11, and NaCl. The indicator of the intensity of trichuriasis invasion when using the improved method was 67.0 ± 17.5 eggs in 1 g of feces and exceeded the number of trichuriasis eggs detected by flotation methods using NaCl – by 2.3 times (P ˂ 0.001), C12H22O11 – by 2.1 times (P ˂ 0.001), NH4NO3 – 1.3 times (P ˂ 0.01), C12H22O11 + NaCl – 1.1 times. The obtained data on the effectiveness of the improved method of coproovoscopy allows us to recommend it for introduction into production for effective and accurate laboratory lifelong diagnosis of trichurosis in sheep.

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Section: Discussionmentioning

confidence: 99%

“…Others, on the contrary, contribute to the delay of egg flotation. In addition, according to the authors, the proposed methods of coproovoscopy should be convenient, inexpensive, and ergonomic (Yevstafieva, 2007a(Yevstafieva, , 2007bCebra & Stang, 2008;Danko & Stybel, 2012;Halat et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of the improved method of lifelong diagnostics trichurosis of sheep

Petrenko

2023

Ukr. Jour. of Vet. and Agr. Sci.

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“…Terentyeva et al (2021) is contributed by the unsanitary conditions of keeping rabbits on unchanged bedding or floors. The optimal air temperature for egg development, according to V. Yevstafieva et al (2021Yevstafieva et al ( , 2022 is +35°C. This coincides with the reports of B.…”

Section: Introductionmentioning

confidence: 99%

Seasonal and age dynamics of passalurosis invasion of rabbits and pathological and histological changes in this nematodosis

Prus¹,

Duda²,

Koreyba³

et al. 2022

Scientific Horizons

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The most common nematode in rabbits is passalurosis, and climate change in Ukraine in recent decades has altered the epizootic manifestation of this parasitosis, which predetermines the relevance of this study. Therefore, the purpose of this paper was to establish the epizootic situation regarding the passalurosis invasion and to investigate the pathological and histological changes in both the large and small intestines of rabbits with a high degree of damage by Passalurus ambiguus. The study used the McMaster method, the improved method of incomplete helminthological dissection according to K.I. Scriabin Private households in the Polissia zone of Ukraine were found to be the most vulnerable in terms of passalurosis infestation, where 37.70-41.67% of sick rabbits were registered. In the seasonal aspect, the highest infection of animals was found in winter (invasion extensiveness (IE) was 35.27%), with a peak in January (IE = 35.29%), the lowest – in summer (IE = 25.79%). Rabbits aged 1 to 2 years had the highest incidence of the pathogen Passalurus ambiguus (82.76%). It was found that with a high degree of damage (invasion intensiveness (II) – 2446.67 ± 422.11 eggs in 1 g of faeces) rabbits with passalurosis had catarrhal and catarrhal-haemorrhagic inflammation, swelling of the mucous membrane of the intestines with dotted or striped haemorrhages. The destruction of the villi of the mucous membrane of the small, caecum, and colon, crypt cells and infiltration by lymphocytes and monocytes, severe swelling of the submucosal base and muscle membrane, and a state of granular dystrophy of all smooth muscle cells of the muscle membrane were established. The detection of seasonal, age-related, and pathological-histological changes in rabbits with passalurosis will allow controlling the degree of infection of animals and promptly taking effective countermeasures, which predetermines the practical value of this study

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Features of the exogenic development of Passalurus ambiguus (Nematoda, Oxyuroidae) at different temperature regimes

et al. 2022

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Passalurosis is a prevalent disease among helminthiases of domestic rabbits. This invasion is caused by the nematode Passalurus ambiguus (Nematoda, Oxyuroidae), which is cosmopolitan and localized in the cecum and colon of rabbits. Passalurosis is highly contagious and capable of unlimited spread, due to the biological characteristics of its pathogen, such as the conditions that ensure maximum preservation of parasites at exogenous stages of their development. Experimental research in the laboratory established the timing of development of P. ambiguus eggs isolated from the gonads of female helminths, depending on temperature regimes and features of their growth and development. According to the morphological features of Passalurus eggs, four stages were distinguished in their exogenous development: zygote, cleavage and formation of blastomeres, formation of larvae and motile larvae. Depending on the cultivation temperature, the duration of embryogenesis ranged from 4 to 9 days, and egg viability ranged from 59.3% to 72.7%. The most favourable temperature regime for the development of P. ambiguus eggs was the temperature of 35 °C, at which 72.7% of eggs with motile larvae were formed within 4 days. At this temperature, the zygote stage lasted for 1 day of cultivation, the stage of cleaving and formation of blastomeres occurred on days 1–2, the stage of larval formation on days 1–3, and the stage of formation of motile larva on days 3–4. At lower temperatures, the term of development of Passalurus eggs increased, and the number of viable eggs decreased. At temperatures of 30 °С and 25 °С, the development of Passalurus eggs took place during 5 and 7 days, respectively, and the viability was 66.7% and 62.7%. At these temperatures, the zygote stage lasted 1–2 and 1–3 days, the stage of cleaving and formation of blastomeres occurred on days 1–3 and 1–4, the stage of larval formation lasted from days 2–4 and 2–6, and the stage of formation of motile larvae took place on days 3–5 and 4–7, respectively. The least favourable temperature for the development of P. ambiguus eggs was the temperature of 20 °С, at which the formation of motile larvae occurred in 9 days, and their viability was only 59.3%. At this temperature, the zygote stage lasted 1–4 days, the stage of cleaving and blastomere formation occurred on days 2–6, and the larval formation stage on days 3–8, and the motile larval stage happened on days 5–9. The growth and development of P. ambiguus eggs was accompanied by significant changes in morphometric parameters, such as the increase in egg width and thinning of egg shell at the egg shell plug. The obtained data will allow preventive measures to be effectively implemented on rabbit farms that are susceptible to pinworms, taking into account the terms of exogenous development of pathogens in different seasons.

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The effectiveness of the proposed method of culturing eggs of nematodes Passalurus ambiguus, parasitic in rabbits

Cited by 3 publications

References 12 publications

Effectiveness of the improved method of lifelong diagnostics trichurosis of sheep

Effectiveness of the improved method of lifelong diagnostics trichurosis of sheep

Seasonal and age dynamics of passalurosis invasion of rabbits and pathological and histological changes in this nematodosis

Features of the exogenic development of Passalurus ambiguus (Nematoda, Oxyuroidae) at different temperature regimes

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