Prevalence, Identification and Antibiotic Susceptibility of &amp;lt;i&amp;gt;Enterococcus&amp;lt;/i&amp;gt; Species Isolated from Chicken and Pigeon Meat in Gazipur Area of Bangladesh

Banik, Avijit; Noor, Mohammad; Akter, Tanzina; Fatema, Kaniz; Abony, Maruf

doi:10.4236/ojmm.2018.83007

Cited by 10 publications

(10 citation statements)

References 12 publications

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“…Collected samples were taken to the (Centre of Excellence) Laboratory, Department of Microbiology of Primeasia University, Dhaka, within the earliest time from purchase for further processing and analysis. 12 Solid food samples were collected in pre-sterilized Stomacher bags (165mm x 150mm x 0.55mm). Fresh juice samples were collected in sterile Duran Bottles and transported in the icebox.…”

Section: Sample Collection and Enrichment Proceduresmentioning

confidence: 99%

“…Presumptive identification of each colony came from biochemical tests and biochemically confirmed isolates were re -confirmed with API 20E kits (BioMerieux, Inc.). 12,19 Twenty -five (25 g) of each sample was homogenized in 225 milliliters of buffered peptone water (Oxoid Ltd, Hampshire, England) and pre-enriched by incubating for overnight at 37 °C. One milliliter of pre-enrichment culture was mixed with 9 -milliliter lactose broth medium (Oxoid Ltd, Hampshire, England) with Durham fermentation tubes and incubated overnight at 37 °C.…”

Section: Microbiological Analysismentioning

confidence: 99%

“…BSTI follows the guidelines of the International Organization for Standardization (ISO) to ensure food safety. 12,13 High-risk pathogens like Campylobacter spp., vero-toxigenic Escherichia coli (VTEC), Salmonella spp., Shigella spp. and V. cholerae should be absent in ready-made foods according to food quality standards.…”

Section: Introductionmentioning

confidence: 99%

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Microbiological Quality of Ready - to - Eat Food from Dhaka, Bangladesh

Banik

Abony

Datta

et al. 2019

Curr Res Nutr Food Sci

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The objective of this research was to assess the microbiological quality of ready-to-eat food available in Dhaka city, Bangladesh, and check the risk factors associated with ingestion of ready-to-eat food from popular public places. This study was conducted in the Center of Excellence in the Department of Microbiology, Primeasia University, Dhaka, Bangladesh from August 2016 to February 2017. Forty-five samples belonging to 18 categories were collected aseptically in triplicates in pre-sterilized zip-lock bags or sterile bottles from Banani area from local street vendors. Samples were transported to and analysed in the Laboratory of Department of Microbiology, Primeasia University according to standard food analysis methods. Total viable count (TVC) and Total coliform count (TCC) were determined by using plate count agar (PCA) andMacConkey agar plates respectively. Antibiogram of the isolated strains was conducted with commercial antibiotics according to the Kirby-Bauer disc diffusion method on Mueller-Hinton agar medium. Identification of the coliforms together with antibiotic-resistance profile showed Escherichia coli, Enterobactersakazaki, Citrobacterfreundii and Salmonella typhimurium were present in various foods. E. coli and S.typhimurium showed increased sensitivity against Ampicillin 10 mg and Sulfamethoxazole 25 mg. The occurrence of antibiotic-resistance potential pathogens in ready-to-eat food poses a considerable health risk to consumers. Public awareness and timely assessment of food safety are needed to avoid the risks of food-borne infection and intoxication from ready-to-eat food.

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Section: Sample Collection and Enrichment Proceduresmentioning

confidence: 99%

Section: Microbiological Analysismentioning

confidence: 99%

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Microbiological Quality of Ready - to - Eat Food from Dhaka, Bangladesh

Banik

Abony

Datta

et al. 2019

Curr Res Nutr Food Sci

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“…All meat samples were slaughtered by the butcher in the meat shop and all eggs and dropping samples were collected from poultry farms in collection areas. All samples were collected in pre-sterilized stomacher bags (165 mm × 150 mm × 0.55 mm) in ice container with aseptic conditions and were transported to the microbiology department (centre of excellence Laboratory) of Primeasia University at the earliest convenience for processing and further assessment [23].…”

Section: Sample Collectionmentioning

confidence: 99%

“…Kirby-Bauer disk diffusion method was performed using Mueller Hinton Agar to determine the antimicrobial activity of antibiotics against isolated S. aureus was measured in vitro [26]. By measuring the diameter of the zone of inhibition allowed the determination of the efficiency of an antibiotic that resulted from the diffusion of the antimicrobial agent into the medium surrounding the disc [23]. Strains were grown in Mueller Hinton broth overnight before inoculation on Mueller Hinton Agar plates.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Akhi

Das

Banik

et al. 2019

MRJI

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Staphylococcus aureus is gram-positive cocci that can cause foodborne illness which can be transmitted by chicken meat, both raw and undercooked; consumption of which may cause infection and/or toxicity in consumers. This current study was conducted for the detection of the prevalence of S. aureus in three types of poultry samples which included chicken meat, chicken eggs and droppings. Samples were aseptically collected from different rural and urban areas of 8 districts of Bangladesh in triplicate collection method which was conducted in the Centre for Excellence, Department of Microbiology, Primeasia University, Dhaka, Bangladesh to detect the Multi Drug-Resistant (MDR) S. aureus. Isolation was done by using 7.5% Sodium Chloride broth for enrichment and Mannitol salt agar after enriching samples in Buffer Peptone water. Antibiogram was done by using Kirby Bauer Method on Muller Hinton agar. The current study revealed 68% (17/25), 84% (21/25) and 52% (13/25) of S. aureus in chicken meat, eggs and dropping, respectively. Around 75.47% isolates (40/53) showed 100% similarity with S. aureus from the generated Dendrogram based on biochemical data. Cefixime (62.67%) showed the highest sensitivity against all of the isolates while Penicillin (86.62%) exhibited the highest resistance. Moreover, 59.62% of isolates were resistant to 5 or more drugs (Multidrug-Resistant). Poultry products such as meat and eggs were contaminated with drug-resistant S. aureus which can cause serious health effect to the consumer. The drug-resistant pathogen in droppings helps to indicate the spread of drug-resistant isolates in the environment. Therefore, proper sanitation measures should be taken to ensure the quality of the products during slaughtering, collecting eggs or disposing of poultry litter.

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Molecular detection and antibiotyping of multi‐drug resistant Enterococcus faecium from healthy broiler chickens in Bangladesh

Roy

Islam

Paul

et al. 2021

Veterinary Medicine & Sci

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Background: Enterococcus faecium is a ubiquitously distributed member of the intestinal microbiota of both humans and animals. Antibiotic resistant E. faecium are a major public health concern.Objectives: This study aimed to detect multi-drug resistant (MDR) E. faecium and their antibiotic resistance genes from broiler chickens in Bangladesh.Methods: A total of 100 faecal samples of healthy broilers were screened by conventional methods and polymerase chain reaction (PCR) to detect E. faecium and their resistance genes. Disk diffusion test was employed to determine antibiotic profiles.Results: By PCR, among 100 samples, 45% [95% confidence interval (CI): 35.62%-54.76%] were positive for E. faecium. Based on antibiogram, all the E. faecium isolates were found resistant to ampicillin, and frequently (93.33%-55.56%) resistant to ceftriaxone, cefotaxime, streptomycin, erythromycin, and imipenem; moderate to lower (26.67%-4.44%) resistance to tetracycline, ciprofloxacin, norfloxacin, chloramphenicol, gentamicin, and vancomycin. Interestingly, 80% (95% CI: 66.18%-89.10%) E. faecium isolates were MDR in nature. In addition, the indices of multiple antibiotic resistance (MAR) ranged from 0.08 to 0.83. By bivariate analysis, high positive significant correlations were observed between resistance profiles of erythromycin and imipenem, ciprofloxacin and norfloxacin, erythromycin and streptomycin, ceftriaxone and cefotaxime, tetracycline and chloramphenicol, and streptomycin and imipenem.Furthermore, the prevalence of resistance genes of E. faecium was 58.33% (tetA), 33.33% (tetB), 35.56% (bla TEM ), 60% (CITM), 13.33% (aadA1), and 12% (SHV). Conclusions:To the best of our knowledge, this is the first study in Bangladesh to detect MDR and MAR E. faecium and their associated resistance genes. The detection of MDR and MAR E. faecium and their corresponding resistance genes from healthyThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Prevalence, Identification and Antibiotic Susceptibility of <i>Enterococcus</i> Species Isolated from Chicken and Pigeon Meat in Gazipur Area of Bangladesh

Abstract: Aims: The aims of this work undertaken to investigate and identify the prevalence of Enterococcus spp isolated from Chicken and pigeon meat and determine the using antibiotics sensitivity against isolates for human safety.

Cited by 10 publications

References 12 publications

Microbiological Quality of Ready - to - Eat Food from Dhaka, Bangladesh

Microbiological Quality of Ready - to - Eat Food from Dhaka, Bangladesh

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Molecular detection and antibiotyping of multi‐drug resistant Enterococcus faecium from healthy broiler chickens in Bangladesh

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