Prevalence, antibiotic resistance and virulence of Enterococcus spp. isolated from traditional cheese types

Jahansepas, Ali; Aghazadeh, Mohammad; Rezaee, Mohammad Ahangarzadeh; Heidarzadeh, Siamak; Mardaneh, Jalal; Mohammadzadeh, Alireza; Pouresmaeil, Omid

doi:10.4314/ejhs.v32i4.17

Cited by 5 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enterococcus faecalis as the predominant species in the tested materials. The present study demonstrated that E. faecalis was the most common in the tested dairy products, which was in agreement with the findings of other studies [ 2 , 9 , 27 , 29 ]. Jahansepas et al even isolated fivefold more E. faecalis (83.33%) than E. faecium (16.67%) from cheese samples in Iran [ 27 ].…”

Section: Discussionsupporting

confidence: 94%

“…The present study demonstrated that E. faecalis was the most common in the tested dairy products, which was in agreement with the findings of other studies [ 2 , 9 , 27 , 29 ]. Jahansepas et al even isolated fivefold more E. faecalis (83.33%) than E. faecium (16.67%) from cheese samples in Iran [ 27 ]. However, one report stated a higher frequency of E. faecium than E. faecalis in Serbian cheeses [ 30 ].…”

Section: Discussionsupporting

confidence: 94%

“…Antimicrobial-resistant E. faecalis strains comprised a higher percentage of all the strains of that species in this study, than resistant E. faecium strains did of all strains of its species. Our observations were in line with previous studies, finding that E. faecalis isolates were more resistant to antimicrobials than E. faecium isolates [ 27 , 33 , 34 ]. All E. faecalis bacteria isolated were not susceptible to quinupristin/dalfopristin because of their intrinsic resistance to this agent, while among E. faecium , six (33%) isolates were resistant to this agent.…”

Section: Discussionsupporting

confidence: 93%

“…are E. faecium, E. faecalis, and E. durans which, excluding E. durans , were claimed in different studies to be the predominant species in most of the milk and dairy products intended for direct consumption [ 4 , 26 ]. Enterococci are a global health problem and are considered opportunistic pathogens and, in fact, these bacteria have been reported to be associated with some human and animal infections [ 27 ]. There are particular challenges in the treatment of enterococcal infections presented by antimicrobial-resistance and virulence genes.…”

Section: Discussionmentioning

confidence: 99%

“…The very low presence of virulence determinants among E. faecium strains in the present study did not disaccord with previous studies, in which their incidence among E. faecium strains was notably lower than that in E. faecalis strains [ 6 ]. Jahansepas et al reported that E. faecium isolates were typically clear of virulence genes, whereas each tested E. faecalis strain revealed multiple profiles of such genes [ 27 ]. Similar results were reported by Yoon et al and Yang et al, in whose research asa1 and gelE were the most prevalent genes possessed by Enterococcus ssp.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Dairy Products: A Potential Source of Multidrug-Resistant Enterococcus faecalis and Enterococcus faecium Strains

Gołaś-Prądzyńska

Łuszczyńska

Rola

2022

Foods

View full text Add to dashboard Cite

This study attempts to present the antimicrobial resistance, virulence and resistance genes of Enterococcus faecalis and Enterococcus faecium isolated from raw goat’s and sheep’s milk and cheese. Strains were identified by PCR. The dominant species was E. faecalis (77.8%) and was most often isolated from raw goat’s milk. The percentage of antimicrobial-resistant E. faecalis isolates was higher than that of E. faecium isolates, the former most frequently resistant to lincomycin (98%), tetracycline (63%) and streptomycin (16%). Fourteen (22.3%) E. faecalis and 2 (11.1%) E. faecium isolates were identified as multidrug-resistant (MDR). All MDR E. faecalis strains also had virulence genes, whereas one of the two E. faecium strains had them. The most prevalent virulence genes in E. faecalis isolates were asa1 (69.8%) and gelE (57.1%). The most prevalent resistance genes found in both bacterial species were tet(M) (43.2%) and vgaA (22.2%). Enterococci from dairy products are confirmed to be a potential source of the spread of antimicrobial resistance, MDR strains, and virulence and resistance genes. This study highlights several aspects of the virulence and pathogenicity of E. faecalis and E. faecium isolated from dairy products—aspects which are indications for their ongoing monitoring.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Dairy Products: A Potential Source of Multidrug-Resistant Enterococcus faecalis and Enterococcus faecium Strains

Gołaś-Prądzyńska

Łuszczyńska

Rola

2022

Foods

View full text Add to dashboard Cite

show abstract

Rapid and specific detection of Enterococcus faecium with an isothermal amplification and lateral flow strip combined method

Wang,

Zhang,

Huo

et al. 2023

Arch Microbiol

View full text Add to dashboard Cite

Comparative study of virulence factor genes, β-hemolysis and biofilm production in invasive and colonizing enterococci

et al. 2023

View full text Add to dashboard Cite

Objectives: In humans, enterococci are among the most important opportunistic pathogens. This study aims to compare invasive isolates obtained from blood cultures of patients with sepsis and endocarditis with colonizing isolates obtained from healthy donors’ stool samples. Methods: A case-by-case assessment was conducted on invasive infection cases to determine whether enterococci were involved in their pathogenesis. They were tested for the presence of virulence factor genes, β-hemolysis on agars supplemented with human and sheep blood, and biofilm forming capacity. Results: Three species of enterococci were identified among invasive isolates: Enterococcus faecalis, Enterococcus faecium, and Enterococcus durans. All endocarditis isolates were biofilm producers. Genes esp, gelE, asa1, ace, hyl, cylB, and cylA were present in 7 (41.2%), 11 (64.7%), 11 (64.7%), 13 (76.5%), 0, 3 (17.6%), and 1 (5.9%) invasive isolate, but none of them could be linked to a particular infection (sepsis or endocarditis). Colonizing isolates proved to have had more virulence factor genes, but the differences were not statistically significant. Members of that group produced a greater amount of biofilm when the ace gene was absent ( p = 0.047). The production of β-hemolysis by noninvasive strains was detected more frequently when agar was supplemented with human blood ( p = 0.021). In general, the presence of either cyl gene on that specific agar was in direct connection with the production of β-hemolysis: cylA ( p = 0.047) or cylB ( p = 0.020). Conclusion: We have been unable to establish any correlation between invasive isolates and any virulence gene carriage and biofilm formation. β-hemolysis was produced significantly more often by colonizing strains when agar had been supplemented with human blood.

show abstract

Prevalence, antibiotic resistance and virulence of Enterococcus spp. isolated from traditional cheese types

Cited by 5 publications

References 32 publications

Dairy Products: A Potential Source of Multidrug-Resistant Enterococcus faecalis and Enterococcus faecium Strains

Dairy Products: A Potential Source of Multidrug-Resistant Enterococcus faecalis and Enterococcus faecium Strains

Rapid and specific detection of Enterococcus faecium with an isothermal amplification and lateral flow strip combined method

Comparative study of virulence factor genes, β-hemolysis and biofilm production in invasive and colonizing enterococci

Contact Info

Product

Resources

About