Prevalence and types of extended-spectrum β-lactamases among urinary Escherichia coli and Klebsiella spp. in New Zealand

Heffernan, Helen; Woodhouse, Rosemary; Pope, Charles E.; Blackmore, Timothy

doi:10.1016/j.ijantimicag.2009.07.014

Cited by 46 publications

(32 citation statements)

References 25 publications

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“…Gentamicin was inactive 19 The amikacin susceptibility rate in this study is lower than that in previous published data in Taiwan. 12 In this study, another potential drug, fosfomycin was actively against ESBL-EC.…”

Section: Discussioncontrasting

confidence: 88%

Antimicrobial susceptibilities of urinary extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin and nitrofurantoin in a teaching hospital in Taiwan

Liu

Lin

et al. 2011

Journal of Microbiology, Immunology and Infection

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Section: Discussioncontrasting

confidence: 88%

Antimicrobial susceptibilities of urinary extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin and nitrofurantoin in a teaching hospital in Taiwan

Liu

Lin

et al. 2011

Journal of Microbiology, Immunology and Infection

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“…This notion is supported by the observation that ESBL-EC had more diverse pulsed field patterns and resistance gene profiles, consistent with more distant epidemiological linkage between isolates. Previous surveys describe similar differences in clonal diversity between these two species [20,21]. Furthermore, we observed no association between any particular hospital and ST131 whereas ESBL-KP in general was associated with Hospital 2 and specific hospitals were associated with specific sequence types, including ST-48.…”

Section: Discussionsupporting

confidence: 60%

Differences in risk-factor profiles between patients with ESBL-producing Escherichia coli and Klebsiella pneumoniae: a multicentre case-case comparison study

Freeman

Rubin

McAuliffe

et al. 2014

Antimicrob Resist Infect Control

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BackgroundGeneric epidemiological differences between extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) and Klebsiella pneumoniae (ESBL-KP), are poorly defined. Nonetheless, defining such differences and understanding their basis could have strategic implications for infection control policy and practice.MethodsBetween 2009 and 2011 patients with bacteraemia due to ESBL-EC or ESBL-KP across all three acute hospitals in the city of Auckland, New Zealand, were eligible for inclusion. Recognised risk factors for ESBL bacteraemia were compared between species in a retrospective case-case study design using multivariate logistic regression. Representative isolates underwent ESBL gene characterisation and molecular typing.Results170 patients and 176 isolates were included in the study (92 patients with ESBL-EC, 78 with ESBL-KP). 92.6% had CTX-Ms. 39% of EC were ST131 while 51% of KP belonged to 3 different STs (i.e. ST20, ST48 & ST1087). Specific sequence types were associated with specific hospitals for ESBL-KP but not ESBL-EC. Variables positively associated with ESBL-EC on multivariate analysis were: community acquired infection (odds ratio [OR] 7.9; 95% CI: 2.6-23.9); chronic pulmonary disease (OR 5.5; 95% CI: 1.5-20.1); and high prevalence country of origin (OR 4.3; 95% CI: 1.6-11.6). Variables negatively associated with ESBL-EC were previous transplant (OR 0.06; 95% CI: 0.007-0.6); Hospital 2 (OR 0.3; 95% CI: 0.1-0.7) and recent ICU admission (OR 0.3; 95% CI: 0.07-0.9).ConclusionsDifferences in risk profiles between patients with ESBL-EC and ESBL-KP suggest fundamental differences in transmission dynamics. Understanding the biological basis for these differences could have implications for infection control practice. Tailoring of infection control measures according to ESBL species may be indicated in some instances.

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“…DNA from E. coli isolates with confi rmed ESBL production was extracted using a genomic DNA purifi cation kit (Thermo Fisher Scientifi c Inc., Massachusetts, USA) and tested by PCR with specifi c primers for the bla TEM , bla SHV and bla CTX-M genes as described elsewhere [22][23][24][25][26][27] E. coli ATCC 35218 (bla TEM-1 ) and K. pneumoniae ATCC 700603 (bla SHV-18 ) were used as positive control strains for bla TEM and bla SHV encoding genes. E. coli NCTC 13461, E. coli NCTC 13462, E. coli NCTC 13463, Enterobacter cloacae NCTC 13464 and K. pneumoniae NCTC 13465 were used as positive controls for the detection of bla CTX-M group 1, group 2, group 8, group 9 and group 25 genes, respectively.…”

Section: Polymerase Chain Reaction and Sequencingmentioning

confidence: 99%

Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep

et al. 2016

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The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples from a total of 200 cattle and 200 sheep were tested and ESBL-producing E. coli was isolated from 31 (15.5%) cattle and three (1.5%) sheep samples using the Clinical and Laboratory Standards Institute's combined disk method. Among the ESBL gene classes detected by PCR, bla CTX-M was the most frequent type, followed by the bla TEM and bla SHV families. ESBL-producing E. coli isolates showed co-resistance to multiple classes of antibiotics including aminoglycosides, phenicols, quinolones, folate pathway inhibitors and tetracyclines. The resistance rates were higher in the cattle isolates than in the sheep isolates. Phylogenetic grouping of the E. coli isolates indicated group A (particularly A 1 ) was the predominant phylogenetic group (19/34, 55.9%), followed by groups B1 (9/34, 26.5%) and D (6/34, 17.6%); none of the isolates belonged to group B2. The study shows that ESBL-producing E. coli isolates exist in the intestinal fl ora of healthy cattle and sheep in the Burdur province of Turkey. This is the fi rst report showing the emergence of CTX-M type ESBL-producing E. coli in sheep farms in Turkey.

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Prevalence and types of extended-spectrum β-lactamases among urinary Escherichia coli and Klebsiella spp. in New Zealand

Cited by 46 publications

References 25 publications

Antimicrobial susceptibilities of urinary extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin and nitrofurantoin in a teaching hospital in Taiwan

Antimicrobial susceptibilities of urinary extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin and nitrofurantoin in a teaching hospital in Taiwan

Differences in risk-factor profiles between patients with ESBL-producing Escherichia coli and Klebsiella pneumoniae: a multicentre case-case comparison study

Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep

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