Prevalence and multi-locus genotyping of Giardia duodenalis from goats in Shaanxi province, northwestern China

Yin, Yanling; Zhang, Huijun; Yuan, Ya-Jie; Tang, Huan; Chen, Dan; Sha, Jie; Wu, Hao-Xian; Wang, Shasha; Zhao, Guanghui

doi:10.1016/j.actatropica.2018.03.013

Cited by 20 publications

(17 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Giardia duodenalis (syn. Giardia intestinalis, Giardia lamblia) is a common gastrointestinal protozoon that causes enteric disease in a variety of animal species and humans [1][2][3][4]. More than 40 species of animals have been reported to be infected with G. duodenalis over the world [2,5].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genotype Distribution of Giardia duodenalis in Rabbits in Shandong Province, Eastern China

Zou

Peng

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Giardia duodenalis is a zoonotic enteric parasite that can infect humans and a number of animal species including rabbits with a worldwide distribution. Infection with G. duodenalis can cause serious public health problems and significant economic losses to animal husbandry. So accurate understanding of the prevalence and genotype distribution of G. duodenalis in rabbits is necessary. In the present study, a total of 616 fecal samples were collected from rabbits in Shandong province, eastern China, and examined in G. duodenalis prevalence and genotypes by nested PCR amplification of β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) gene loci of G. duodenalis. Sixty-nine (11.2%) of the examined rabbit fecal samples were G. duodenalis-positive. Of them, the prevalence of G. duodenalis is 8.4% (41/490) in Rizhao city and 22.2% (28/126) in Weihai city. Breeds, region, and feeding modes were highly correlated with G. duodenalis infection in rabbits. Moreover, three genotypes

show abstract

Section: Introductionmentioning

confidence: 99%

Prevalence and Genotype Distribution of Giardia duodenalis in Rabbits in Shandong Province, Eastern China

Zou

Peng

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…China has the largest population of sheep and goats around the world [16]. Thus far, a number of studies have reported the infection of G. duodenalis in sheep and goats in China, with prevalences of 1.82–13.11% [13, 17–23] and 0.95–27.78% [17, 20, 24–30], respectively. Although asymptomatic infection of G. duodenalis commonly occurs in sheep and goats [31], an outbreak of giardiasis in a sheep farm located in central Italy had G. duodenalis -infected lambs showing malabsorption, weight loss, decrease of feed conversion ratio, malodorous and poorly formed greenish feces [32], causing significant economic loss to sheep on this farm.…”

Section: Introductionmentioning

confidence: 99%

“…Although asymptomatic infection of G. duodenalis commonly occurs in sheep and goats [31], an outbreak of giardiasis in a sheep farm located in central Italy had G. duodenalis -infected lambs showing malabsorption, weight loss, decrease of feed conversion ratio, malodorous and poorly formed greenish feces [32], causing significant economic loss to sheep on this farm. Assemblages A, B and E have been identified in sheep and goats in China, with assemblage E as the most frequently detected assemblage [3, 13, 30]. The identification of zoonotic assemblages A and B suggests that sheep and goats with G. duodenalis infections are likely to be the source of human giardiasis.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and multilocus genotyping of Giardia duodenalis in black-boned sheep and goats in southwestern China

et al. 2019

Self Cite

View full text Add to dashboard Cite

BackgroundGiardia duodenalis is an important intestinal protozoan infecting both humans and animals, causing significant public health concern and immeasurable economic losses to animal husbandry. Sheep and goats have been reported as common reservoirs of G. duodenalis, but only a limited amount of information is available for native breeds of these small ruminants in China. The present study investigated the prevalence and multilocus genotypes of G. duodenalis in black-boned sheep and goats, two important native breeds in Yunnan Province, southwestern China.MethodsFecal samples were collected from 336 black-boned goats and 325 black-boned sheep from five counties (Meishui, Shanshu, Shilin, Yongsheng and Nanping) of Yunnan Province and the genomic DNA was extracted from these feces. The prevalence of G. duodenalis was determined by the nested PCR targeting the β-giardin (bg) gene. The assemblages and multilocus genotypes (MLGs) were investigated based on analyses of three genetic loci, i.e. bg, glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi).ResultsGiardia duodenalis infection was detected in both black-boned sheep and goats, and the prevalence of G. duodenalis in black-boned sheep (21.8%, 71/325) was significantly higher (χ2 = 36.63, df = 1, P < 0.001) than that in black-boned goats (4.8%, 16/336). Significant differences in prevalence were also observed in goats and sheep from different counties (χ2 = 39.83, df = 4, P < 0.001) and age groups (χ2 = 97.33, df = 3, P < 0.001). Zoonotic assemblage A and animal-specific assemblage E were identified in both black-boned sheep and goats with the latter as the predominant assemblage. Based on sequences obtained from the three genetic loci (bg, gdh and tpi), 16 MLGs were obtained in black-boned sheep and goats, including 15 MLGs in assemblage E and one MLG in assemblage A.ConclusionsOur results not only extended the host range of G. duodenalis, but also revealed high genetic variations in G. duodenalis assemblages. The findings of the present study also provide baseline data for preventing and controlling G. duodenalis infection in black-boned sheep and goats in Yunnan Province.

show abstract

“…G. duodenalis prevalence was significantly different among the study areas ( χ 2=10.933, df =4, P < 0.05), between different age groups ( χ 2=5.208, df =1, P < 0.05), and between different genders ( χ 2=1.615, df =1, P > 0.05). The G. duodenalis prevalence in Yunnan black goats was higher than that (2.9%) in goats in Heilongjiang province [ 22 ], but lower than that in goats in Anhui (6.3%) [ 23 ], Shaanxi (7.9%) [ 25 ], and Henan provinces (12.7%) [ 24 ], China. The G. duodenalis prevalence in Yunnan black goats was markedly lower than in goats in Greece (40.4%) [ 34 ], Spain (42.0%) [ 20 ], Uganda (40.7%) [ 35 ], and Belgium (35.8%) [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…Yunnan province is the fifth largest producer of goats in China [ 19 ], and about 10 million goats are raised each year. Many previous studies have reported G. duodenalis infection in goats in other countries with prevalence ranging from 2.9 to 35.8% [ 20 , 21 ], but only limited investigations have been conducted in goats in China, with the prevalence ranging from 2.9 to 7.1% [ 22 – 25 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Multilocus Genotyping of Giardia duodenalis in Yunnan Black Goats in China

Xie

Zou

Chen

et al. 2018

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Giardia duodenalis is an important zoonotic parasite which can parasitize in the intestines of humans and various animals. However, the information about the prevalence and genetic diversity of G. duodenalis in goats in China is limited. It is yet to be known whether Yunnan black goats, a unique goat breed in subtropical Yunnan province, southwestern China, are infected with G. duodenalis. Thus, a total of 907 fecal samples were collected from Yunnan black goats in five regions in Yunnan province, to estimate the prevalence and genotypes of G. duodenalis using a PCR-based approach. The G. duodenalis prevalence is 4.2% (38/907) in Yunnan black goats by nested amplification of the β-giardin (bg) gene, and the genotypes are identified as assemblage E, with 5 novel subtypes (E11-E15). Multilocus sequence typing revealed that 11, 18, and 38 samples were amplifiable on tpi (triose phosphate isomerase), gdh (glutamate dehydrogenase), and bg locus, respectively, and identified three novel multilocus genotypes (MLGs): MLGE9-MLGE11. To our knowledge, this is the first report of G. duodenalis prevalence and genotypes in Yunnan black goats in China, which extended the host range of G. duodenalis and provided basic data for controlling G. duodenalis infection in Yunnan black goats.

show abstract

Prevalence and multi-locus genotyping of Giardia duodenalis from goats in Shaanxi province, northwestern China

Cited by 20 publications

References 36 publications

Prevalence and Genotype Distribution of Giardia duodenalis in Rabbits in Shandong Province, Eastern China

Prevalence and Genotype Distribution of Giardia duodenalis in Rabbits in Shandong Province, Eastern China

Occurrence and multilocus genotyping of Giardia duodenalis in black-boned sheep and goats in southwestern China

Occurrence and Multilocus Genotyping of Giardia duodenalis in Yunnan Black Goats in China

Contact Info

Product

Resources

About