Prevalence and molecular typing of Coxiella burnetii in bulk tank milk in Belgian dairy goats, 2009–2013

Boarbi, Samira; Mori, Marcella; Rousset, Élodie; Sidi-Boumedine, Karim; Esbroeck, Marjan Van; Frétin, David

doi:10.1016/j.vetmic.2014.01.025

Cited by 40 publications

(44 citation statements)

References 29 publications

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“…These samples were derived from Belgian caprine isolates (CbBEC1, CbBEC2, and CbBEC4) (20,24) and bovine isolates (CbBEB1 and another isolate derived from bovine fetal products previously characterized by the original laboratory as SNP genotype 2) (24) and were subjected to a blind analysis.…”

Section: Methodsmentioning

confidence: 99%

“…In Belgium, the disease is enzootic in the domestic ruminant population (20)(21)(22), while sporadic cases are detected in humans (23). In this work, the aim was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms, using a rapid and easy technique (SNP analysis) that can allow comparison of the results with already existing studies conducted in Belgium (20,24) and in The Netherlands (14).…”

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confidence: 99%

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Single Nucleotide Polymorphism Genotyping and Distribution of Coxiella burnetii Strains from Field Samples in Belgium

Pozzo

Renaville

Martinelle

et al. 2016

Appl Environ Microbiol

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The genotypic characterization of Coxiella burnetii provides useful information about the strains circulating at the farm, region, or country level and may be used to identify the source of infection for animals and humans. The aim of the present study was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms using a single nucleotide polymorphism (SNP) technique. Direct genotyping was applied to different samples (bulk tank milk, individual milk, vaginal swab, fetal product, and air sample). Besides the well-known SNP genotypes, unreported ones were found in bovine and caprine samples, increasing the variability of the strains found in the two species in Belgium. Moreover, multiple genotypes were detected contemporarily in caprine farms at different years of sampling and by using different samples. Interestingly, certain SNP genotypes were detected in both bovine and caprine samples, raising the question of interspecies transmission of the pathogen.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Single Nucleotide Polymorphism Genotyping and Distribution of Coxiella burnetii Strains from Field Samples in Belgium

Pozzo

Renaville

Martinelle

et al. 2016

Appl Environ Microbiol

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“…La prévalence troupeau dans le cheptel caprin de décem-bre 2009 à mars 2013 variait entre 6,3 et 12,1 %. Malgré la circulation de la souche de type néerlandais (CbNL01), le nombre de notifications de cas humains belges était très bas (Boarbi et al 2014). …”

Section: En Europeunclassified

Coxiella burnetii, agent de la fièvre Q

Boarbi¹,

Frétin²,

Mori³

2016

Can. J. Microbiol.

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Résumé : La fièvre Q est une zoonose de répartition mondiale excepté la Nouvelle-Zélande. Elle est due à une bactérie intracellulaire : Coxiella burnetii. Cette infection passe souvent inaperçue car la manifestation principale de la forme aiguë de la maladie est un syndrome pseudogrippal spontanément résolutif. C'est l'épidémie qui a eu lieu aux Pays-Bas qui a attiré l'attention sur cette maladie qui était moins étudiée auparavant. Cette synthèse résume la description de la bactérie (taxonomie, cycle intracellulaire et génomique) mais également la maladie (description, diagnostic, pathogénie et épidémiologie). Enfin, la vaccination de l'humain et des animaux est également analysée. Mots-clés : Coxiella burnetii, fièvre Q, épidémiologie, description, génomique.Abstract: Q fever is a zoonosis of worldwide distribution with the exception of New Zealand. It is caused by an intracellular bacterium, Coxiella burnetii. The disease often goes underdiagnosed because the main manifestation of its acute form is a general self-limiting flu-like syndrome. The Dutch epidemics renewed attention to this disease, which was less considered before. This review summarizes the description of C. burnetii (taxonomy, intracellular cycle, and genome) and Q fever disease (description, diagnosis, epidemiology, and pathogenesis). Finally, vaccination in humans and animals is also considered.

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“…In bovines, C. burnetii DNA is detected more often in milk than in vaginal mucus after abortion. The shedding of the pathogen in milk was described for its taking one of two different profiles (5). The first profile is active and associated with the SNP1 or CbNLO1-like MLVA genotype of C. burnetii, and the second one is discontinuous and is caused by the other genotypes (5).…”

Section: Shedding Of C Burnetiimentioning

confidence: 99%

“…The shedding of the pathogen in milk was described for its taking one of two different profiles (5). The first profile is active and associated with the SNP1 or CbNLO1-like MLVA genotype of C. burnetii, and the second one is discontinuous and is caused by the other genotypes (5). According to the data from the European Food Safety Authority (EFSA), Q fever is present in a herd when abortions or reproductive disorders are observed, about 50% of animals are seropositive, and C. burnetii DNA is detected in vaginal mucus or placentas (9).…”

Section: Shedding Of C Burnetiimentioning

confidence: 99%

Prevalence of Coxiella Burnetii in Dairy Herds - Diagnostic Methods and Risk to Humans - A Review

Szymańska-Czerwińska

Niemczuk

Mitura

2014

Bulletin of the Veterinary Institute in Pulawy

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Q fever is a zoonotic disease caused by Coxiella burnetii. The main source of infection are ruminants (cattle, sheep, and goats). C. burnetii is excreted via birth products, vaginal mucus, milk, and faeces. Raw milk is considered useful for epidemiological examinations of animals and evaluation of infection dynamics at the herd level. This article summarises data on prevalence studies on C. burnetii in bulk-tank milk in different European countries with the means of serological tests and PCR. It also summarises the results of studies to evaluate the actual risk of disease transmission to humans through consumption of raw milk. Moreover, the available diagnostic tools for detection C. burnetii infection are presented.

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Prevalence and molecular typing of Coxiella burnetii in bulk tank milk in Belgian dairy goats, 2009–2013

Cited by 40 publications

References 29 publications

Single Nucleotide Polymorphism Genotyping and Distribution of Coxiella burnetii Strains from Field Samples in Belgium

Single Nucleotide Polymorphism Genotyping and Distribution of Coxiella burnetii Strains from Field Samples in Belgium

Coxiella burnetii, agent de la fièvre Q

Prevalence of Coxiella Burnetii in Dairy Herds - Diagnostic Methods and Risk to Humans - A Review

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