Prevalence and Molecular Detection of Dengue Virus in 2013 Outbreak in KPK and Punjab, Pakistan

Shahid, Muhammad; Amin, Irum; Afzal, Samia; Fatima, Zareen; Zahid, Sadia; Ashraf, Usman; Idrees, Muhammad

doi:10.17582/journal.pjz/2017.49.3.sc4

Cited by 10 publications

(7 citation statements)

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“…Our results are in accordance with previous reports suggesting that the age group 15‐45 years is typical of infection in the area, and the serotypes 2 and 3 remain the most common in previous outbreaks in Pakistan as summarized in Table 2. Various studies published in the last decade have reported DENV‐2 and DENV‐3 as predominant serotype except Humayoun and colleagues, which found DENV 4 as prevalent serotype 4,21‐28 …”

Section: Discussionmentioning

confidence: 99%

“…At present, the main method to prevent the transmission of DENV is the control of mosquito populations 2 . Over the past decade, the country has faced several outbreaks in which thousands of people have been infected and they even lost their lives 4,21‐28 . In Punjab, a populous province that shares a border with KP, in 2011 a serious epidemic affected over 50 000 people.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan

Khan

Danish

Khan³

et al. 2020

Clinical Laboratory Analysis

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Background Dengue is a viral disease, transmitted by infected Aedes aegypti and Aedes albopictus female mosquitoes. Worldwide, 96 million infections were estimated in 2010. The dengue virus comprises four distinct serotypes (DENV‐1, DENV‐2, DENV‐3, and DENV‐4) which belong to the genus Flavivirus. Determining the serotypes during dengue outbreaks is crucial for its effective management in terms of diagnostics improvement and polyvalent vaccine development. The aim of the present study is to determine the prevalence rate of dengue virus serotypes in the samples collected from patients during the 2017 outbreak in Khyber Pakhtunkhwa, Pakistan. Methods A total of 800 ELISA‐positive samples were collected, of which 513 (290 males, 223 females) samples were confirmed positive by PCR. Results Out of 513, 25 were found serotype 1 (5%), 196 were serotype 2 (38%), 192 were serotype 3 (37%), 56 were serotype 4 (11%), and 44 (8%) were found to have mix serotypes. Conclusion We can conclude that serotypes 2 and 3 of dengue virus were the predominated serotypes of dengue virus in the 2017 outbreak in Peshawar, capital city of Khyber Pakhtunkhwa, Pakistan.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan

Khan

Danish

Khan³

et al. 2020

Clinical Laboratory Analysis

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“…Further, the patients were documented with the symptoms of dengue infection such as fever (100%), inner bleeding, and enlarged liver 43.96% as mentioned in Table 6 . According to a previous study, liver wounds in dengue disease could be due to the direct influence of the virus or host immunity with liver cells, which could become a facet for vascular leakage inside the liver 30 .…”

Section: Discussionmentioning

confidence: 99%

Incidence of Dengue fever, serotypes, clinical features, and laboratory markers: a case study of 2019 outbreak at district Shangla, KP, Pakistan

Rehman¹,

Anwar²,

Tayyab³

et al. 2022

Afr H. Sci.

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Background: Dengue is a widely spread mosquito-borne infection in humans, which in recent decades declared is public health problem globally. The dengue virus contains 4 different serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) which belong to the genus Flavivirus. Aims: A descriptive experimental study was conducted to determine the epidemiology, types of Dengue serotypes, clinical features, laboratory probe, and markers for primary diagnosis of dengue virus infection in hospitalized patients. Methodology: A total of 691 suspects were diagnosed from August to October 2019 in district Shangla KP, Pakistan. Serological tests were used for nonstructural protein-1 antigen (NS1), and antibodies (immunoglobulin-M (IgM) & Immunoglobulin-G (IgG)) while real-time PCR was used to confirm the cases. The data was statistically analyzed using IBM-SPSS Statistics 20 version. Results: The dengue virus infection was more prevalent in the male group (68.09%) than the female group (31.1%). A large number of patients were from rural areas (63.5%) while from urban areas were (36.4%), whereas Besham tehsil was found the most affected compared to other regions. The most prevalent serotype observed in our study was DENV-3 (56.60%) while DENV-4 was the least prevalent serotype (1.88%). Among the age-wise analysis of dengue-virus-infected individuals, the age group of 19–37 years (64.07%) was found the most affected group. The month-wise analysis revealed that the highest number of infections (49.8%) were recorded in September. Significant differences were noticed among blood parameters. Conclusion: The possible reasons for the dengue overwhelming in the study area could be less or lack of awareness particularly regarding the transmission of viral infections, improper sewage management, and no effective vector control strategies that lead the dengue outbreaks in the study population. Keywords: Dengue; Outbreak; DENV; real-time PCR; RNA Virus; Pakistan.

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“…The death rate caused by DHF and DSS is approximately 5%. Patients who have recovered from dengue fever could be immune to the dengue serotype that had infected them 17 …”

Section: Introductionmentioning

confidence: 99%

“…Patients who have recovered from dengue fever could be immune to the dengue serotype that had infected them. 17 District Bannu is located in Khyber Pakhtunkhwa (KP) province Pakistan. Every year dengue cases from this area are observed.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological and hematological investigation of dengue virus infection

Anwar

Ullah

Aziz

et al. 2022

Microbiology and Immunology

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Dengue is a mosquito-borne viral illness that infects humans. For the past few decades, it has been declared a global public health problem. The current study, conducted at the district headquarter hospital (DHQ) Bannu between June to September 2018, was based on the seroprevalence of antibodies against dengue virus serotypes and their hematological parameters among the patients. A total of 1738 individuals suspected of having dengue were diagnosed through NS1, IgG, and IgM antibodies and RT-PCR techniques. Of all the samples, 716 (41.19%) were found to be positive for dengue. A higher infection rate was found in males (65.92%) compared with females (34.07%). The most affected age group was 16-40 years, whereas the most affected tehsil was Bannu, where the DENV-3 serotype was prevalent. The rare serotype (DENV-4) was found in 1% of cases. Symptoms including fever (100%), myalgia (100%), headache (61.31%), vomiting (34.63%), and rashes were common among the dengue patients. However, the mild cases showed fewer clinical signs compared with the severely infected cases. The study also revealed a significant association (P < 0.05) between hematological parameters and dengue infection, showing a significant decrease in TC, eosinophils, neutrophils, and platelets and a significant increase in monocytes and lymphocytes. Based on the current report, it is concluded that patients with the above symptoms and hematological changes may have an increased probability of dengue and should be kept under observation to separate dengue-positive patients and to enhance the treatment process.

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Prevalence and Molecular Detection of Dengue Virus in 2013 Outbreak in KPK and Punjab, Pakistan

Cited by 10 publications

References 0 publications

Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan

Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan

Incidence of Dengue fever, serotypes, clinical features, and laboratory markers: a case study of 2019 outbreak at district Shangla, KP, Pakistan

Epidemiological and hematological investigation of dengue virus infection

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