Prevalence and molecular characterization of human group C rotaviruses in Hungary

Bányai, Krisztiàn; Jiang, Baoming; Bogdan, A. T.; Horváth, Balázs; Jakab, Ferenc; Meleg, Edina; Martella, Vito; Magyari, Lili; Melegh, Béla; Szücs, György

doi:10.1016/j.jcv.2006.08.017

Cited by 34 publications

(30 citation statements)

References 27 publications

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“…An alternative hypothesis to explain the presence of animal-like strains in the communal sewage is that they might infect humans. The usually low detection rate of group C rotaviruses in childhood gastroenteritis (around 1% for children less than 5 years of age [3,5,41]) contrasts with reports of the relative abundance of antibodies against the virus in the same age group, as nearly 20% to 30% of children develop an antibody response to group C rotavirus by 5 years of age (14,21,37). An apparent increase in the serological prevalence of group C rotavirus has been observed among people of rural communities, an increase which has been linked to enhanced exposure to animals (13).…”

Section: Vol 74 2008 Group C Rotavirus In Communal Sewage 3397mentioning

confidence: 86%

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Detection and Quantification of Group C Rotaviruses in Communal Sewage

Meleg

Bányai

Martella

et al. 2008

Appl Environ Microbiol

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7 ; median, 6.9 ؋ 10 5 genome equivalents per liter influent sewage) and appeared to display seasonal activity over the study period, whereas animal strains appeared to circulate throughout the year at much lower average titers (bovine strains mean, 9.9 ؋ 10 4 ; median, 3.0 ؋ 10 4 ; porcine strains mean, 3.9 ؋ 10 4 ; median, 3.1 ؋ 10 4 genome equivalents per liter influent sewage). Our findings suggest that monitoring of communal sewage may provide a good surrogate for investigating the epidemiology and ecology of group C rotaviruses in humans and animals.

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Section: Vol 74 2008 Group C Rotavirus In Communal Sewage 3397mentioning

confidence: 86%

“…The incidence of group C rotavirus diarrhea in children is generally considered low (3,6,27,35,38,41). The low number of identified cases does not permit us to understand the ecology and epidemiology, including the source and mode of transmission of group C rotavirus, in a variety of settings and areas in the world.…”

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confidence: 99%

Detection and Quantification of Group C Rotaviruses in Communal Sewage

Meleg

Bányai

Martella

et al. 2008

Appl Environ Microbiol

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“…However, molecular analysis of human GCRV strains would not seem to support this theory, as all of the human GCRVs form a closely related group, suggesting a strong host-species restriction (2,34). These inconsistencies may be a result of the limited amount of sequence data available for animal strains.…”

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confidence: 99%

“…Human infection by GCRV has been associated with both sporadic episodes and large outbreaks of gastroenteritis in all age groups and appears to be globally distributed (1,2,5,6,18,24,25,34,38,41,43). Large-scale epidemiological studies have revealed that the prevalence may range from 0.6% to 6.8% (2,24), and GCRVs are regarded as emerging human pathogens.…”

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confidence: 99%

Detection and Characterization of Group C Rotaviruses in Asymptomatic Piglets in Ireland

2008

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Group C rotaviruses are important human enteric pathogens that have also been detected in a variety of mammalian species, including pigs. Group C rotaviruses have been identified in piglets with diarrhea, but their ecology remains to be elucidated. By screening of 292 fecal samples collected from 4-to 5-week-old asymptomatic pigs from four herds in Ireland between 2005 and 2007, 13 (4.4%) samples tested positive by reverse transcription-PCR for group C rotavirus. Group A rotaviruses were also detected in 19 samples but not in conjunction with group C viruses. The gene encoding the major group C neutralization antigen, the outer capsid protein VP7, was sequenced. The majority of the strains were very closely related to each other (>99% amino acid [aa] identity) and were characterized as genogroup G1 since they were genetically related to the prototype porcine strain Cowden (92.6% aa identity). Conversely, two strains (1GA/05/Cork/Ire and 281/07/ Dublin/Ire) were characterized as genogroup G6 since they displayed the highest identity (89.2 to 94.0% aa) to porcine G6 strains (43/06-22-like). Unexpectedly, one such G6 strain, 1GA/05/Cork/Ire, lacked the 4-aa insertion in the VP7 variable region VR8 found in all the other G6 group C rotaviruses. This study provides evidence that porcine group C rotavirus may be detected not infrequently in asymptomatic piglets. In addition, it provides evidence that, unlike the human viruses, porcine group C rotaviruses display broad genetic heterogeneity, which may pose a challenge for the development of prophylactic tools.

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“…This has led to the hypothesis that human RVCs, unlike human RVAs, may have undergone a recent bottleneck event in their evolution . Alternatively, RVCs would be less prone to inter-species transmission than RVAs, thus limiting the extent of their genetic/antigenic diversification in the human host (B anyai et al, 2006;Martella et al, 2010). Also, in this study, evidence for zoonotic transmission of RVC strains was not observed, by comparing human and animal RVC sequences available in the NCBI databases.…”

Section: Discussionmentioning

confidence: 80%

Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment

Medici

Tummolo

Martella

et al. 2016

Journal of General Virology

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Group C rotaviruses (RVC) are enteric pathogens of humans and animals. Whole-genome sequences are available only for few RVCs, leaving gaps in our knowledge about their genetic diversity. We determined the full-length genome sequence of two human RVCs (PR2593/ 2004 and PR713/2012), detected in Italy from hospital-based surveillance for rotavirus infection in 2004 and 2012. In the 11 RNA genomic segments, the two Italian RVCs segregated within separate intra-genotypic lineages showed variation ranging from 1.9 % (VP6) to 15.9 % (VP3) at the nucleotide level. Comprehensive analysis of human RVC sequences available in the databases allowed us to reveal the existence of at least two major genome configurations, defined as type I and type II. Human RVCs of type I were all associated with the M3 VP3 genotype, including the Italian strain PR2593/2004. Conversely, human RVCs of type II were all associated with the M2 VP3 genotype, including the Italian strain PR713/2012. Reassortant RVC strains between these major genome configurations were identified. Although only a few full-genome sequences of human RVCs, mostly of Asian origin, are available, the analysis of human RVC sequences retrieved from the databases indicates that at least two intra-genotypic RVC lineages circulate in European countries. Gathering more sequence data is necessary to develop a standardized genotype and intragenotypic lineage classification system useful for epidemiological investigations and avoiding confusion in the literature.

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Prevalence and molecular characterization of human group C rotaviruses in Hungary

Cited by 34 publications

References 27 publications

Detection and Quantification of Group C Rotaviruses in Communal Sewage

Detection and Quantification of Group C Rotaviruses in Communal Sewage

Detection and Characterization of Group C Rotaviruses in Asymptomatic Piglets in Ireland

Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment

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