Detection and Quantification of Group C Rotaviruses in Communal Sewage

Meleg, Edina; Bányai, Krisztiàn; Martella, Vito; Jiang, Baoming; Kocsis, Béla; Kisfali, Péter; Melegh, Béla; Szücs, György

doi:10.1128/aem.02895-07

Cited by 39 publications

(28 citation statements)

References 41 publications

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“…In a recent study in Brazil, rotavirus group C was detected in 32 of 35 (91.4 %) influent samples and 20 of 35 (57.1 %) effluent samples. The detection rates of group C rotavirus varied among the treatment plants; 17 of 17 raw samples and 9 of 17 treated samples were positive in plant A, while the detection rate was 5 of 6 for both the influent and effluent samples from plant B and 4 of 6 for both the raw and treated sewage samples from plant C. In plant D, 6 of 6 raw samples and 3 of 6 treated samples were positive (Meleg et al 2008).…”

Section: Discussionmentioning

confidence: 91%

Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment

2015

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The objective of this study is to compare the prevalence of rotaviruses groups A and C in Egyptian children and aquatic environment. From 110 stool specimens of children with acute diarrhea and using RT-PCR, 35 samples (31.8 %) were positive for human rotavirus group A and 15 samples (13.6 %) were positive for human rotavirus group C. From 96 samples collected from Zenin wastewater treatment plant over a 2-year period (November 2009-October 2011) and using RT-PCR, rotavirus group A was detected in (4/24) 16.7 %, (5/24) 20.8 %, (4/24) 16.7 %, and (4/24) 16.7 %, while rotavirus group C was detected in (2/24) 8.3 %, (3/24) 12.5 %, (3/24) 12.5 %, and (0/24) 0 % in raw sewage, after primary sedimentation, after secondary sedimentation, and after final chlorination, respectively. Moreover, from 96 samples collected from El-Giza water treatment plant over a 2-year period (November 2009-October 2011), rotavirus group A was detected in (7/24) 29.2 %, (6/24) 25 %, (5/24) 20.8 %, and (3/24) 12.5 %, while rotavirus group C was detected in (3/24) 12.5 %, (1/24) 4.2 %, (1/24) 4.2 %, and (0/24) 0 % in raw Nile water, after sedimentation, after sand filtration, and after final chlorination, respectively. Using SYBR Green real-time RT-PCR, the number of human rotavirus group A genome or infectious units was higher than rotavirus group C. VP6 sequence analysis of the RT-PCR positive rotavirus group C samples revealed that four clinical specimens and three environmental samples showed similar sequences clustered with Moduganari/Human Nigerian strain AF 325806 with 98 % homology, and two clinical specimens and one environmental sample showed similar sequences clustered with Dhaka CB/Human Bangladesh strain AY 754826 with 97 % homology.

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Section: Discussionmentioning

confidence: 91%

Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment

2015

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“…A number of studies on RVC from all over the world by several researchers prove that this group is as important as RVA and causes severe acute gastroenteritis in human and swine (Rahman et al 2005;Meleg et al 2008;Moon et al 2011;Marthaler et al 2013;Suzuki et al 2014). In a routine electrophoretic screening survey for RVA in swine population, we found an atypical migration pattern of genomic RNA segments from a piglet fecal specimen (Figure 1), resembling established electrophoretic pattern (4-3-2-2) of RVC (Desselberger 2014).…”

Section: Discussionmentioning

confidence: 99%

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Kattoor

Saurabh

Malik

et al. 2017

Veterinary Quarterly

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Background: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog. Objective: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes. Methods: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed. Results: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population. Conclusion:The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

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“…Thus, raw sewage is likely to contain pathogenic organisms similar to those in the original human excreta (Meleg et al, 2008;Kargar et al, 2009;Mueller et al, 2009;Barril et al, 2010). In that way, the detection and quantification of the viral load in untreated wastewater could mirror the intensity and genotype diversity of rotavirus circulating in the community.…”

Section: Introductionmentioning

confidence: 98%

Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity

Barril

Fumian

Prez

et al. 2015

Environmental Research

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Detection and Quantification of Group C Rotaviruses in Communal Sewage

Cited by 39 publications

References 41 publications

Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment

Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity

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