Prevalence and molecular characterization of porcine Picobirnavirus in piglets of North East Region of India

Kylla, Hosterson; Dutta, Tapan Kumar; Roychoudhury, P.; Malik, Yashpal Singh; Mandakini, Rajkumari; Subudhi, P.K.

doi:10.1007/s11250-016-1210-7

Cited by 10 publications

(10 citation statements)

References 19 publications

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“…Maternal immunity might also play an important role in resisting the infection in piglets, particularly in indigenous pigs. In the same region in a study of diarrheagenic pathogens, reported a higher prevalence rate of Picobirnavirus and STEC in crossbreed than indigenous piglets population [24,46]. In our study, samples collected during different seasons showed that coinfection of enteric pathogens was found to be most common during the summer (June-August).…”

Section: Discussionsupporting

confidence: 56%

“…Detection of Rotavirus Groups A and C was performed by targeting VP7 gene [22] and VP6 gene [23], respectively. For the detection of Picobirnavirus genogroup I (GGI) [24] and GGII [25], specific primers were used. Details of oligonucleotides and PCR conditions used in the present study are given in Table-1 [22][23][24][25][26][27][28][29].…”

Section: Detection Of Rotavirus and Picobirnavirusmentioning

confidence: 99%

“…In case of Salmonella isolates, the detection of enterotoxin (stn) [27], invasive (invA) [28], and plasmid efficacy fimbrial (pef) [29] genes was carried out by specific PCR assay in a thermal cycler (Eppendorf, Germany). Oligonucleotides and PCR conditions used in the study are depicted in Table-1 [22][23][24][25][26][27][28][29].…”

Section: Detection Of Bacterial Virulence Genes By Pcrmentioning

confidence: 99%

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Coinfection of diarrheagenic bacterial and viral pathogens in piglets of Northeast region of India

Kylla¹,

Dutta

Roychoudhury

et al. 2019

Vet World

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Aim: This study aimed to study the prevalence of the coinfection of enteric bacterial and viral pathogens, namely Escherichia coli, Salmonella, Rotavirus, and Picobirnavirus from fecal samples of pre-weaned piglets in Northeast region of India. Materials and Methods: A total of 457 fresh fecal samples were collected from piglets under 9 weeks old during 2013-2015 from organized (n=225) and unorganized (n=232) farms of Manipur, Meghalaya, Mizoram, and Nagaland. Samples were collected from diarrheic (n =339) and non-diarrheic (n=118) piglets including local indigenous (n=130) and crossbreed (n=327) piglets in different seasons during the study period. The samples were processed for the isolation of E. coli and Salmonella and detection of their putative virulence genes by polymerase chain reaction (PCR). Samples were also processed for the detection of Rotavirus and Picobirnavirus by RNA-polyacrylamide agarose gel electrophoresis and reverse transcriptase-PCR (RT-PCR). Results: A total of 11 (2.40%) samples were found positive for two or more coinfecting enteric bacterial and viral pathogens. All the 11 positive fecal samples were recovered from diarrheic piglets. Salmonella Typhimurium (enterotoxin, stn gene) and Picobirnavirus genogroup 1 were found to be more frequent as coinfecting agents. Coinfection was recorded higher in unorganized (3.87%) compared to organized farm (0.88%). Again, higher detection was recorded in crossbreed (2.75%) than local indigenous piglets (1.53%). The occurrence of coinfection was found to be more common during summer (4.68%) followed by winter (2.27%) season. Conclusion: The present study highlighted the significance of E. coli, Salmonella, Rotavirus, and Picobirnavirus as important diarrheagenic pathogens causing coinfection in piglets in Northeast region of India. Probably, this is the first systematic study of the coinfection of four important diarrheagenic bacterial and viral agents associated with piglet diarrhea in India.

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Section: Discussionsupporting

confidence: 56%

Section: Detection Of Rotavirus and Picobirnavirusmentioning

confidence: 99%

Section: Detection Of Bacterial Virulence Genes By Pcrmentioning

confidence: 99%

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Coinfection of diarrheagenic bacterial and viral pathogens in piglets of Northeast region of India

Kylla¹,

Dutta

Roychoudhury

et al. 2019

Vet World

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“…The phylogenetic analysis revealed genetic similarity to different porcine and human GG-I PBVs from different geographical regions. In 2016, Porcine PBV infection was reported from North Eastern Region of India with strains having varied diversity and were not found to be closely associated with any other Indian isolates of PBVs so far [ 21 ]. The study also claims that PBV infections are higher in acute summer and winter seasons compared to spring and autumn seasons with high prevalence recorded in cross breed pigs in contrast to local breeds.…”

Section: Introductionmentioning

confidence: 99%

Epidemiologic Status of Picobirnavirus in India, A Less Explored Viral Disease

Malik¹,

Sircar²,

Saurabh³

et al. 2018

TOVJ

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Since the unexpected discovery of picobirnaviruses (PBV) in 1988, they have been reported in many animals including mammals and birds, which comprises both terrestrial and marine species. Due to their divergent characteristics to other viral taxa they are classified into a new family Picobirnaviridae. Although their pathogenicity and role in causing diarrhea still remains a question since they have been discovered in symptomatic and asymptomatic cases both. Recent studies employing state-of-art molecular tools have described their presence in various clinical samples, like stool samples from different mammals and birds, respiratory tracts of pigs and humans, sewage water, different foods, etc. Furthermore, their epidemiological status from different parts of the world in different hosts has also increased. Due to their diverse host and irregular host pattern their role in causing diarrhea remains alien. The heterogeneity nature can be ascribed to segmented genome of PBV, which renders them prone to continuous reassortment. Studies have been hampered on PBVs due to their non-adaptability to cell culture system. Here, we describe the molecular epidemiological data on PBVs in India and discusses the overall status of surveillance studies carried out till date in India.

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“…As reported by Ganesh et al[4] and Fregolente et al[2], PBV prevalence in different mammalian hosts ranges from 12.5 to 14.3%. Particularly in pigs, a recent study conducted by Kylla et al[9] found 11.5% of PBV prevalence as the most significant rate in diarrheal animals.Because PBV can infect humans, employees from farm A were sampled to investigate the presence of the virus in their feces. Fifteen feces samples were collected and analyzed by RT-PCR.…”

mentioning

confidence: 96%

Detection and partial molecular characterization of <i>Picobirnavirus</i> in swine from the state of Minas Gerais, Brazil

Rosado

Santos

Silva

et al. 2020

The Journal of Veterinary Medical Science

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Picobirnavirus (PBV) is a small two-segmented double-stranded RNA (dsRNA) virus that has been identified in diarrheic feces of a large range of animal hosts, including humans. For this reason, PBV has been recognized as an opportunistic agent of gastrointestinal disease. Even under these circumstances, there is a lack of studies regarding this pathogen. Not outstanding, in Brazil, the single description of the PBV occurrence in pigs was provided in the 1980s. Hence, this study aimed to verify the PBV occurrence in Brazilian swine farms and to perform molecular characterization of the identified strains. High genetic variability was found in the analyzed sequences. Further studies comprehending the infection of swine by PBV in Brazilian herds should be performed to provide more accurate information on its epidemiology and to discuss the role of the virus in gastrointestinal diseases.

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Prevalence and molecular characterization of porcine Picobirnavirus in piglets of North East Region of India

Cited by 10 publications

References 19 publications

Coinfection of diarrheagenic bacterial and viral pathogens in piglets of Northeast region of India

Coinfection of diarrheagenic bacterial and viral pathogens in piglets of Northeast region of India

Epidemiologic Status of Picobirnavirus in India, A Less Explored Viral Disease

Detection and partial molecular characterization of <i>Picobirnavirus</i> in swine from the state of Minas Gerais, Brazil

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