Prevalence and Molecular Characterization of Hydatid Cyst Isolates from Cattle in Egypt

Abbas, Ibrahim; Al-Kappany, Y. M.; Al-Araby, Moustafa

doi:10.3923/ajava.2016.794.804

Cited by 10 publications

(7 citation statements)

References 59 publications

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“…Another study performed in Cairo reported that 18.4% of the examined cattle were infected by CE ( 70 ). In contrast, the occurrence rate obtained in the present study was higher than those reported in cattle slaughtered in abattoirs in Assiut, Mansoura, and other provinces of Upper Egypt, where hydatid cysts were noted in 0.4, 0.068, and 0.004% of the examined animals, respectively ( 25 , 71 , 72 ). The discrepancy in the occurrence of CE in camels and cattle in the present study vs. those reported in several previous studies may be attributed to several factors, such as hygienic practices during slaughter, sex and age of the slaughtered animals, the method of detection, the geographic location, and various climatic conditions ( 25 , 45 , 73 , 74 ).…”

Section: Discussioncontrasting

confidence: 97%

“…In accordance with the studied potential variable factors, the statistical analysis revealed that the number and type of cysts, organ involved, and fertility and viability of hydatid cysts were not significantly associated with the occurrence of the of CE in camels or cattle. The present study also showed that CE were only detected in the lungs of camels, while cysts occurred mainly in the lungs of cattle and only one cyst was reported in the liver of cattle, which is consistent with several previous reports, mainly from Egypt ( 69 , 70 , 72 ). However, some previous reports revealed that the parasite exhibited single-organ involvement, although the involvement of two organs has also been recorded, which is apparently associated with the specific geographic regions and strain of the parasite ( 25 , 76 , 77 ).…”

Section: Discussionsupporting

confidence: 93%

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Epidemiological, Morphometric, and Molecular Investigation of Cystic Echinococcosis in Camel and Cattle From Upper Egypt: Current Status and Zoonotic Implications

et al. 2021

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Cystic echinococcosis has been considered one of the major parasitic zoonoses which is associated with severe economic losses. The present study was undertaken to investigate the occurrence, organ distribution, cyst fertility, and viability of cystic echinococcosis in slaughtered camels and cattle from various abattoirs in Assiut Governorate, Egypt. The work also involved morphological, morphometric, and molecular identification of the parasite. The occurrence of hydatid cysts was investigated in total number of 100 lungs of camels and 574 liver and lungs of cattle admitted to three slaughterhouses at Assiut Governorate, Egypt. Moreover, several individual variable factors, including organ involvement, age, sex, and hydatid cyst characteristics, were studied to identify their possible association with the occurrence of the disease. Genomic DNA was extracted from the hydatid cysts, followed by molecular identification of the parasite through amplification of ribosomal DNA internal transcribed spacer (ITS) regions. Hydatid cysts were found in 6 camels (6%) out of 100 inspected camels, while 5 hydatid cysts (0.87%) were detected in a total number of 574 cattle examined. The parasite was detected exclusively in lungs of camels, while lungs were the main organ infected by the parasite in cattle and one hydatid cyst was found in the liver (0.17%). In camel, 66.7, 16.65, and 16.65%of detected cysts were fertile, sterile, and calcified, respectively, while in cattle, these percentages were 60, 20, and 20%, respectively. None of the studied variable factors were significantly associated with the occurrence of the disease in camels, with the exception that all cysts were found in the lung. Conversely, we found a significant association (P < 0.05) between the age and sex of the slaughtered cattle and the occurrence of hydatid cysts. In this respect, the rate of infection was higher in female cattle and those cattle more than 5 years (P < 0.05). The morphological, morphometric, and molecular studies confirmed the presence of the parasite. Taken together, our results concluded that camels and cattle play a potential role in maintaining the transmission cycle of this zoonotic parasite.

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Section: Discussioncontrasting

confidence: 97%

Section: Discussionsupporting

confidence: 93%

Epidemiological, Morphometric, and Molecular Investigation of Cystic Echinococcosis in Camel and Cattle From Upper Egypt: Current Status and Zoonotic Implications

et al. 2021

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“…There was a difference in the rate of prevalence of CE in different areas; the prevalence was high in the Lakki Marwat district as compared to Bannu and Karak. Similar trends of variation in the prevalence have been reported by other researchers [ 16 , 24 – 26 , 30 ], suggesting that CE prevalence varies from region to region or even in different regions/parts of the same country. Most probably this variation in the prevalence may be associated with geographical distribution, social and cultural activities of the local people, and a good sanitary system.…”

Section: Discussionsupporting

confidence: 88%

Cystic echinococcosis: an emerging zoonosis in southern regions of Khyber Pakhtunkhwa, Pakistan

et al. 2021

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Background Cystic echinococcosis (CE) is one of the principal causes of economic loss to the livestock industry because of its morbidity and mortality of food-producing animals and condemnation of important visceral organs. Pakistan being an agricultural country having an extensive livestock sector, is mostly practiced by poor people, which has a fundamental role in the economy. The present study was aimed to conduct a cross-sectional survey and PCR based confirmation of Echinococcus granulosus in sheep, goats, cows, and buffaloes from southern regions (three districts: Lakki Marwat, Bannu, and Karak) of Khyber Pakhtunkhwa, Pakistan. During the study, a total of 2833 animals were examined randomly including; sheep (n = 529), goats (n = 428), cows (n = 1693), and buffaloes (n = 183). Hydatid cysts were collected and examined for the presence of protoscoleces using microscopy. Detection of DNA was performed by using PCR and two mitochondrial genetic markers namely; NAD-1 and COX-1 were amplified. Results The overall prevalence of CE was found to be (9%) among the examined animals. The hydatid cyst infection was highly prevalent in buffaloes (12%), followed by sheep (10%), cows (9%), and goats (5.1%). Cystic echinococcosis was more prevalent (10%; 96/992) in district Lakki Marwat followed by district Bannu (9%; 112/1246) and Karak (7%; 39/595). Female animals were more likely to be infected with CE (11.6%) than male animals (5.3%) (p = 0.001). Similarly, the infection was higher in the older group of animals as compared to younger (p = 0.001). Mostly (52.2%; n = 129) of hydatid cysts were found in the liver, while (64.4%; n = 159) cysts of the infected animals were infertile. PCR based identification confirmed the presence of E. granulosus sensu stricto (s.s) in the study area. Conclusion Cystic echinococcosis was found to be highly prevalent in southern regions of Khyber Pakhtunkhwa and could be a potential threat to human health. Moreover, molecular sequencing and phylogenetic analyses should be carried out in future to identify the prevailing genotype (s) of E. granulosus s.s.

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“… Amer et al, 2015 , identified that G1 and G6 from sheep in Egypt. Abbas et al, 2016 , detected E. granulosus s. s . (G1) and E. ortleppi (G5) from cattle slaughtered at the Mansoura abattoir, Egypt.…”

Section: Discussionmentioning

confidence: 99%

“…In Egypt, most samples collected from camels slaughtered at the Toukh abattoir (Qalyubia Governorate) were identified as E. canadensis (G6), and a few of these strains were identified as E. ortleppi (G5), isolated from newly imported Sudanese camels ( Abdel Aziz et al, 2016 ). Abbas et al, 2016 reported, for the first time, a cattle HC formed by the G1 sheep strain and another cyst formed by the G5 strain (a cattle strain). Additionally, the G1 sheep strain was detected in both Egyptian camel and sheep ( Amer et al, 2015 ).…”

Section: Introductionmentioning

confidence: 97%

Genetic characterization of hydatid cysts of different intermediate hosts

et al. 2020

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SummaryCystic echinococcosis is an important cosmopolitan parasitic zoonosis that causes public health and economic problems in Egypt. The present study was undertaken to identify genotypes of hydatid cyst (HC) DNA isolated from different animal isolates and to identify the genotype of secondary hydatid cysts (HCs) developed in rabbits experimentally infected with camel HC for detection of any genetic mutation. In the present study, we extracted DNA from the germinal layers of 8 HCs collected from 3 camels, 1 cattle, 1 sheep and 3 donkeys in addition to 3 secondary HCs collected from rabbits experimentally infected with camel HC. PCR amplification of the ITS1 gene of all examined samples showed an amplified DNA band at 1115 bp. The partial nucleotide sequences of the ITS1 gene of all isolates were aligned and compared with the reference sequences of the genotypes G1–G8 in GenBank. The camel and rabbit samples were identified as Echinococcus canadensis genotype 6 (G6), while the cattle and sheep samples belonged to E. granulosus sensu stricto (G1). The donkey isolates belonged to E. equines (G4). Alignment of the ITS1 partial nucleotide sequences of the camel HCs and rabbit secondary HCs isolates with the G6 partial nucleotide sequence in GenBank was performed. Both camel HCs and rabbit secondary HCs isolates exhibited the same sequence identity matrix, which indicated the absence of mutation in the rabbit secondary HCs. It can be concluded that camel and rabbit samples were identified as E. canadensis (G6), the cattle and sheep samples belonged to E. granulosus sensu stricto (G1) and donkey isolates belonged to E. equines (G4). No mutation occurred during HCs transmission from camel to rabbit.

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Prevalence and Molecular Characterization of Hydatid Cyst Isolates from Cattle in Egypt

Cited by 10 publications

References 59 publications

Epidemiological, Morphometric, and Molecular Investigation of Cystic Echinococcosis in Camel and Cattle From Upper Egypt: Current Status and Zoonotic Implications

Epidemiological, Morphometric, and Molecular Investigation of Cystic Echinococcosis in Camel and Cattle From Upper Egypt: Current Status and Zoonotic Implications

Cystic echinococcosis: an emerging zoonosis in southern regions of Khyber Pakhtunkhwa, Pakistan

Genetic characterization of hydatid cysts of different intermediate hosts

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