Genetic characterization of hydatid cysts of different intermediate hosts

Abstract

SummaryCystic echinococcosis is an important cosmopolitan parasitic zoonosis that causes public health and economic problems in Egypt. The present study was undertaken to identify genotypes of hydatid cyst (HC) DNA isolated from different animal isolates and to identify the genotype of secondary hydatid cysts (HCs) developed in rabbits experimentally infected with camel HC for detection of any genetic mutation. In the present study, we extracted DNA from the germinal layers of 8 HCs collected from 3 camels, 1 cattle, 1 sheep and 3 donkeys in addition to 3 secondary HCs collected from rabbits experimentally infected with camel HC. PCR amplification of the ITS1 gene of all examined samples showed an amplified DNA band at 1115 bp. The partial nucleotide sequences of the ITS1 gene of all isolates were aligned and compared with the reference sequences of the genotypes G1–G8 in GenBank. The camel and rabbit samples were identified as Echinococcus canadensis genotype 6 (G6), while the cattle and sheep samples belonged to E. granulosus sensu stricto (G1). The donkey isolates belonged to E. equines (G4). Alignment of the ITS1 partial nucleotide sequences of the camel HCs and rabbit secondary HCs isolates with the G6 partial nucleotide sequence in GenBank was performed. Both camel HCs and rabbit secondary HCs isolates exhibited the same sequence identity matrix, which indicated the absence of mutation in the rabbit secondary HCs. It can be concluded that camel and rabbit samples were identified as E. canadensis (G6), the cattle and sheep samples belonged to E. granulosus sensu stricto (G1) and donkey isolates belonged to E. equines (G4). No mutation occurred during HCs transmission from camel to rabbit.

Cystic echinococcosis in humans and animals in Egypt: An epidemiological overview

“…include G1 ( E. granulosus ( s.s. ) from camel, sheep, buffalo and cattle), G4 (from donkey), G5 (from camel and cattle), G6 (from camel, pig, sheep, buffalo, cattle and Norway rat), and G7 (from pig) ( Fig. 1 ) ( Abd El Baki et al., 2009 ; Abdel Aaty et al, 2012 ; Aboelhadid et al., 2013 ; Khalifa et al., 2014 ; Alam-Eldin et al., 2015 ; Amer et al., 2015 ; Abbas, 2016 ; Abbas et al., 2016 ; Abdel Aziz & Meghanawy, 2016 ; Abdel-Moein & Hamza, 2016 ; Desouky et al., 2017 ; Mousa et al., 2020 ). Given that the majority of camels slaughtered at abattoirs in Cairo and Qualyobia governorates are imported from Sudan where G5 infections have been reported in camels ( Omer et al., 2010 ; Ibrahim et al., 2011 ; Ahmed et al, 2013 ), it is likely to identify the same genotype in Egypt.…”

“…(2009) G4 4 Donkeys nad 1 Giza Desouky et al. (2017) G4 3 Donkeys ITS1 na Mousa et al. (2020) G6 6 Camels ITS1 na Mousa et al.…”

“…(2017) G4 3 Donkeys ITS1 na Mousa et al. (2020) G6 6 Camels ITS1 na Mousa et al. (2020) G1 1 Cattle ITS1 na Mousa et al.…”

“…(2020) G1 1 Cattle ITS1 na Mousa et al. (2020) G1 1 Sheep ITS1 na Mousa et al. (2020) Abbreviation : na, not available.…”

Protoscolices Antigens From Hydatid Cyst of Human and Animal Origin for Diagnosis of Cystic Echinococcosis

“…Thus, imaging methods (radiological and ultrasound) combined with the immunological methods were used to confirm and support clinical signs and symptoms of HC patients (Sunita et al, 2011). Al-so, molecular characterization was used to diagnose hydatdosis in different hosts (Mousa et al, 2020). ELISA was one of the commonest used tests as being cheap and easily simple, but sensitivity and accuracy depended on characters and stability of used antigen (Golassa et al, 2011;Zhang et al, 2012).…”