Prevalence and Molecular Characterization of Ampicillin-Resistant Enterobacteriaceae Isolated from Traditional Egyptian Domiati Cheese

Hammad, Ahmed M.; Ishida, Yojiro; Shimamoto, Tadashi

doi:10.4315/0362-028x-72.3.624

Cited by 24 publications

(15 citation statements)

References 29 publications

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“…Although the RTE products had been produced at different places and sold at different commercial sites, no diversity was detected among the bla TEM gene family, which evidences the great spread of bla TEM-1 in these RTE types of food. Unlike the results obtained in this study, Hammad et al (2009) found diversity in b-lactamases from ampicillin-resistant enterobacterial isolates obtained from traditional Egyptian Domiati cheese. In addition to the high prevalence of different types of SHV family, these isolates also contained CTX-M-14, OXY-1, OXA-1, and CMY-4.…”

Section: Antimicrobial Susceptibility Testingcontrasting

confidence: 99%

In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods

Amador

Fernandes

Duarte

et al. 2010

World J Microbiol Biotechnol

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The principal aim of this study was to investigate the possibility of transference to Escherichia coli of b-lactam resistance genes found in bacteria isolated from ready-to-eat (RTE) Portuguese traditional food. From previous screenings, 128 b-lactam resistant isolates (from different types of cheese and of delicatessen meats), largely from the Enterobacteriaceae family were selected and 31.3% of them proved to transfer resistance determinants in transconjugation assays. Multiplex PCR in donor and transconjugant isolates did not detect bla CTX , bla SHV and bla OXY , but bla TEM was present in 85% of them, while two new TEMs (TEM-179 and TEM-180) were identified in two isolates. The sequencing of these amplicons showed identity between donor and transconjugant genes indicating in vitro plasmid DNA transfer. These results suggest that if there is an exchange of genes in natural conditions, the consumption of RTE foods, particularly with high levels of Enterobacteriaceae, can contribute to the spread of antibiotic resistance.

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Section: Antimicrobial Susceptibility Testingcontrasting

confidence: 99%

In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods

Amador

Fernandes

Duarte

et al. 2010

World J Microbiol Biotechnol

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show abstract

“…Year of collection City, country/region (one), qnrB6 (one), and qnrB20 (one) [186] qnrA, qnrB, qnrS isolates [191] qnrA, qnrB, qnrS, aac ( [194] qnrA, qnrB, qnrC, qnrS, aac(6 0 )-Ibcr, qepA (4),…”

Section: Clinical Relevance Of Pmqrmentioning

confidence: 99%

Plasmid-mediated quinolone resistance: an update

Rodríguez-Martínez

Velasco

Pascual

et al. 2011

Journal of Infection and Chemotherapy

257

190

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“…In addition, OXA-1 was shown to be responsible for high level resistance to ampicillin in S. Typhimurium isolates recovered from fish in India (Ruiz et al, 1999). Recently, a study conducted in Japan showed that among a series of ampicillin-resistant enterobacterial isolates recovered from traditional Egyptian Domiati cheese, some were producing OXA-1 (Hammad et al, 2009). …”

Section: Esbl And/or Ampc Encoding Resistance Genesmentioning

confidence: 99%

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

2011

EFSA Journal

240

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The potential contribution of food-producing animals or foods to public health risks by ESBL and/or AmpCproducing bacteria is related to specific plasmid-mediated ESBL and/or AmpC genes encoded by a number of organisms. The predominant ESBL families encountered are CTX-M, TEM, and SHV; the predominant AmpCfamily is CMY. The most common genes associated with this resistance in animals are bla CTX-M-1 (the most commonly identified ESBL), and bla CTX-M-14 , followed by bla and bla Among the genes encoding AmpC-type β-lactamases, bla CMY-2 is the most common.The bacterial species most commonly identified with these genes are Escherichia coli and non-typhoidal Salmonella. ESBL/AmpC transmission is mainly driven by integrons, insertion sequences, transposons and plasmids, some of which are homologous in isolates from both food-production animals and humans. Cefotaxime is used as the drug of choice for optimum detection of bla ESBL and/or bla AmpC genes. The preferred method for isolation of ESBL-and/or AmpC-producers is screening on selective agar preceded by selective enrichment in a broth.The establishment of risk factors for occurrence of ESBL/AmpC-producing bacteria is particularly complicated by the data unavailability or lack of its accuracy. The use of antimicrobials is a risk factor for the selection and spread of resistant clones, resistance genes and plasmids. Since most ESBL-and AmpC-producing strains carry additional resistances to other commonly-used veterinary drugs, generic antimicrobial use is a risk factor for ESBL/AmpC and it is not restricted specifically to the use of cephalosporins. An additional risk factor is extensive trade of animals in EU MS. There are no data on the comparative efficiency of individual control options in reducing public health risks caused by ESBL and/or AmpC-producing bacteria related to food-producing animals. Prioritisation is complex, but it is considered that a highly effective control option would be to stop all uses of cephalosporins/systemically active 3 rd /4 th generation cephalosporins, or to restrict their use (use only allowed under specific circumstances). As co-resistance is an important issue, it is also of high priority to decrease the total antimicrobial use in animal production in the EU. KEY WORDSResistance, ESBLs, AmpC, occurrence, transmission, control options, public health microbiology. SUMMARYFollowing a request from the European Commission, the Panel on Biological Hazards (BIOHAZ) was asked to deliver a Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum beta (β)-lactamases (ESBL) and/or AmpC β-lactamases (AmpC) in food and foodproducing animals. In particular, the Panel was asked: (i) to propose a definition of the ESBL-and/or AmpC-producing bacterial strains and genes relevant for public health and linked to food-producing animals or food borne transmission; (ii) to review the information on the epidemiology of acquired resistance to broad spectrum cephalosporins including the genes coding for ...

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Prevalence and Molecular Characterization of Ampicillin-Resistant Enterobacteriaceae Isolated from Traditional Egyptian Domiati Cheese

Cited by 24 publications

References 29 publications

In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods

In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods

Plasmid-mediated quinolone resistance: an update

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

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